Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Key Bio Nano Consulting partner develops novel nanolitre scale technique for determining protein stability and ligand-binding affinity

Schematic of the microcapillary technique for label-free protein analysis
Schematic of the microcapillary technique for label-free protein analysis

Abstract:
BNC to provide commercial access to new technique enabling evaluation of low expressing proteins without the need for fluorescent tags

Key Bio Nano Consulting partner develops novel nanolitre scale technique for determining protein stability and ligand-binding affinity

UK | Posted on August 25th, 2010

Bio Nano Consulting (BNC), specialists in bio and nanotechnology partnerships between the academic and industrial sectors, announces the availability of a new technique that screens low-yielding proteins, facilitating the evaluation of both protein denaturation and protein:drug interactions.

This novel method, pioneered by researchers from the UCL Department of Biochemical Engineering and the London Centre for Nanotechnology, in collaboration with Cambridge and Sussex Universities, is applicable to any free solution protein:drug interaction. The technique applies microfluidics, using just nanolitres of sample - coupling protein stability and drug affinity analysis to microfluidic devices for sample preparation. In a paper recently published in the industry journal Protein Science, the researchers established a new nanolitre scale technique in micro-capillaries to measure intrinsic protein fluorescence and obtain accurate protein denaturation curves at equilibrium - for the full article please click here.

For the first time, free energies of protein unfolding were determined by the innovative method and used to determine the affinity of a drug - the immunosuppressant rapamycin - to a protein, the cellular immunophilin FKBP12. In addition, the microcapillary technique was used to measure the interaction energy between rapamycin and the Phe99 residue of FKBP-12 from a double mutant cycle analysis. The method utilised combinations of two different protein mutations to study the molecular interactions within a given protein.

Until now, the modus operandi for protein analysis often meant either too much of the precious biological or compound materials were consumed in large sample volumes, or that chemical labelling with fluorescent tags was required to achieve measurements at submicrolitre volumes.

Commenting on the ground-breaking technique Dr David Sarphie, BNC's CEO, said: "We are delighted to be able to offer access to this innovative technique developed by scientific researchers at UCL and LCN to our clients. Indeed, this new method has vast implications and uses in high throughput, low cost platforms for drug discovery, protein engineering and protein formulation applications in the pharmaceutical industry."

He added: "Moreover, this technology marks a further development in the implementation of our corporate strategy to forge industry collaborations with leading academic and scientific organisations that possess the know-how to assess novel drug targets. Indeed, we look forward to combining our project management expertise with access to the technology and novel technique concerned to develop screening systems in the drug discovery market."

####

About Bio Nano Consulting
Bio Nano Consulting is a joint venture of Imperial College London and University College London that specialises in delivering innovative solutions to industry across the life sciences and technology sectors. We are the first consultancy in Europe to focus on the intersection between bio and nanotechnology, providing product development and strategic consultancy, project management and access to state of the art instrumentation and world-leading researchers.

For more information, please click here

Contacts:
Tristan Jervis or Alex Heeley on
00 44 207 861 3838

Copyright © Bio Nano Consulting

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Tissue regeneration using anti-inflammatory nanomolecules August 22nd, 2014

A breakthrough in imaging gold nanoparticles to atomic resolution by electron microscopy August 22nd, 2014

Ultra-short pulse lasers & Positioning August 21st, 2014

Malvern’s Dr Alan Rawle talks TLAs in plenary lecture at Particulate Systems Analysis conference August 21st, 2014

Academic/Education

SEMATECH and Newly Merged SUNY CNSE/SUNYIT Launch New Patterning Center to Further Advance Materials Development: Center to Provide Access to Critical Tools that Support Semiconductor Technology Node Development August 7th, 2014

Oxford Instruments Asylum Research and the Center for Nanoscale Systems at Harvard University Present a Workshop on AFM Nanomechanical and Nanoelectrical Characterization, Aug. 21-22 August 6th, 2014

University of Manchester selects Anasys AFM-IR for coatings and corrosion research July 30th, 2014

Haydale Announces Collaboration Agreement with Swansea University’s Welsh Centre for Printing and Coatings (WCPC) July 12th, 2014

Nanomedicine

Tissue regeneration using anti-inflammatory nanomolecules August 22nd, 2014

Ultra-short pulse lasers & Positioning August 21st, 2014

Nanotechnology Helps Production of Super Adsorbent Polymers August 21st, 2014

Newly-Developed Nanobiosensor Quickly Diagnoses Cancer August 20th, 2014

Announcements

Tissue regeneration using anti-inflammatory nanomolecules August 22nd, 2014

A breakthrough in imaging gold nanoparticles to atomic resolution by electron microscopy August 22nd, 2014

Malvern’s Dr Alan Rawle talks TLAs in plenary lecture at Particulate Systems Analysis conference August 21st, 2014

Water window imaging opportunity: A new theoretical study elucidates mechanisms that could help in producing coherent radiations, ultimately promoting high-contrast imaging of biological samples August 21st, 2014

Tools

A breakthrough in imaging gold nanoparticles to atomic resolution by electron microscopy August 22nd, 2014

Hiden Release New Gas Analysis Catalogue August 21st, 2014

Wyatt Technology’s 24th International Light Scattering Colloquium to Highlight Developments in Applications and Characterization of Nanoparticles August 21st, 2014

Ultra-short pulse lasers & Positioning August 21st, 2014

Nanobiotechnology

The channel that relaxes DNA: Relaxing DNA strands by using nano-channels: Instructions for use August 20th, 2014

Сalculations with Nanoscale Smart Particles August 19th, 2014

Interaction between Drug, DNA for Designing Anticancer Drugs Studied in Iran August 17th, 2014

Scientists fold RNA origami from a single strand: RNA origami is a new method for organizing molecules on the nanoscale. Using just a single strand of RNA, this technique can produce many complicated shapes. August 14th, 2014

Research partnerships

A breakthrough in imaging gold nanoparticles to atomic resolution by electron microscopy August 22nd, 2014

Сalculations with Nanoscale Smart Particles August 19th, 2014

Promising Ferroelectric Materials Suffer From Unexpected Electric Polarizations: Brookhaven Lab scientists find surprising locked charge polarizations that impede performance in next-gen materials that could otherwise revolutionize data-driven devices August 18th, 2014

Nano Bonds Increase Raw Strength of Fireproof Concretes August 18th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE