Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Rensselaer Polytechnic Institute Researchers Develop Coating That Safely Kills MRSA on Contact

Image credit: Rensselaer/Ravindra C.Pangule
Image credit: Rensselaer/Ravindra C.Pangule

Abstract:
Building on an enzyme found in nature, researchers at Rensselaer Polytechnic Institute have created a nanoscale coating for surgical equipment, hospital walls, and other surfaces which safely eradicates methicillin resistant Staphylococcus aureus (MRSA), the bacteria responsible for antibiotic resistant infections.

Rensselaer Polytechnic Institute Researchers Develop Coating That Safely Kills MRSA on Contact

Troy, NY | Posted on August 17th, 2010

"We're building on nature," said Jonathan S. Dordick, the Howard P. Isermann Professor of Chemical and Biological Engineering, and director of Rensselaer's Center for Biotechnology & Interdisciplinary Studies. "Here we have a system where the surface contains an enzyme that is safe to handle, doesn't appear to lead to resistance, doesn't leach into the environment, and doesn't clog up with cell debris. The MRSA bacteria come in contact with the surface, and they're killed."

In tests, 100 percent of MRSA in solution were killed within 20 minutes of contact with a surface painted with latex paint laced with the coating.

The new coating marries carbon nanotubes with lysostaphin, a naturally occurring enzyme used by non-pathogenic strains of Staph bacteria to defend against Staphylococcus aureus, including MRSA. The resulting nanotube-enzyme "conjugate" can be mixed with any number of surface finishes — in tests, it was mixed with ordinary latex house paint.

Unlike other antimicrobial coatings, it is toxic only to MRSA, does not rely on antibiotics, and does not leach chemicals into the environment or become clogged over time. It can be washed repeatedly without losing effectiveness and has a dry storage shelf life of up to six months.

The research, led by Dordick and Ravi Kane, a professor in the Department of Chemical and Biological Engineering at Rensselaer, along with collaboration from Dennis W. Metzger at Albany Medical College, and Ravi Pangule, a chemical engineering graduate student on the project, has been published in the July edition of the journal ACS Nano, published by the American Chemical Society.

Dordick said the nanotube-enzyme coating builds on several years of previous work embedding enzymes into polymers. In previous studies, Dordick and Kane discovered that enzymes attached to carbon nanotubes were more stable and more densely packed when embedded into polymers than enzymes alone.

"If we put an enzyme directly in a coating (such as paint) it will slowly pop out," Kane said. "We wanted to create a stabilizing environment, and the nanotubes allow us to do that."

Having established the basics of embedding enzymes into polymers, they turned their attention to practical applications.

"We asked ourselves — were there examples in nature where enzymes can be exploited that have activity against bacteria?" Dordick said. The answer was yes and the team quickly focused on lysostaphin, an enzyme secreted by non-pathogenic Staph strains, harmless to humans and other organisms, capable of killing Staphylococcus aureus, including MRSA, and commercially available.

"It's very effective. If you put a tiny amount of lysostaphin in a solution with Staphylococcus aureus, you'll see the bacteria die almost immediately," Kane said.

Lysostaphin works by first attaching itself to the bacterial cell wall and then slicing open the cell wall (the enzyme's name derives from the Greek "lysis" meaning "to loosen or release").

"Lysostaphin is exceptionally selective," Dordick said. "It doesn't work against other bacteria and it is not toxic to human cells."

The enzyme is attached to the carbon nanotube with a short flexible polymer link, which improves its ability to reach the MRSA bacteria, said Kane.

"The more the lysostaphin is able to move around, the more it is able to function." Dordick said.

They successfully tested the resulting nanotube-enzyme conjugate at Albany Medical College, where Metzger maintains strains of MRSA.

"At the end of the day we have a very selective agent that can be used in a wide range of environments — paints, coating, medical instruments, door knobs, surgical masks — and it's active and it's stable," Kane said. "It's ready to use when you're ready to use it."

The nanotube-enzyme approach is likely to prove superior to previous attempts at antimicrobial agents, which fall into two categories: coatings that release biocides, or coatings that "spear" bacteria.

Coatings that release biocides — which work in a manner similar to marine anti-fouling paint — pose harmful side-effects and lose effectiveness over time as their active ingredient leaches into the environment.

Coatings that spear bacteria — using amphipatic polycations and antimicrobial peptides — tend to clog, also losing effectiveness.

The nanotube-lysostaphin coating does neither, said Dordick.

"We spent quite a bit of time demonstrating that the enzyme did not come out of the paint during the antibacterial experiments. Indeed, it was surprising that the enzyme worked as well as it did while remaining embedded near the surface of the paint," Dordick said.

The enzyme's slicing or "lytic" action also means that bacterial cell contents disperse, or can be removed by rinsing or washing the surface.

Kane also said MRSA are unlikely to develop resistance to a naturally occurring enzyme.

"Lysostaphin has evolved over hundreds of millions of years to be very difficult for Staphylococcus aureus to resist," Kane said. "It's an interesting mechanism that these enzymes use that we take advantage of."

####

For more information, please click here

Contacts:
Mary L. Martialay
Phone: (518) 276-2146

Copyright © Rensselaer Polytechnic Institute

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Industrial Nanotech, Inc. to Publish PCAOB Audited Financials July 31st, 2014

Nanostructured metal-oxide catalyst efficiently converts CO2 to methanol: Highly reactive sites at interface of 2 nanoscale components could help overcome hurdle of using CO2 as a starting point in producing useful products July 31st, 2014

Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method July 31st, 2014

Pressure probing potential photoelectronic manufacturing compound July 31st, 2014

Academic/Education

University of Manchester selects Anasys AFM-IR for coatings and corrosion research July 30th, 2014

Haydale Announces Collaboration Agreement with Swansea University’s Welsh Centre for Printing and Coatings (WCPC) July 12th, 2014

STFC takes delivery of the 100th Hitachi Tabletop SEM in the UK July 3rd, 2014

Innovation Management and the Emergence of the Nanobiotechnology Industry July 1st, 2014

Nanotubes/Buckyballs

SouthWest NanoTechnologies Names NanoSperse as A SWeNT Certified Compounder July 29th, 2014

A new way to make microstructured surfaces: Method can produce strong, lightweight materials with specific surface properties July 29th, 2014

UCF Nanotech Spinout Developing Revolutionary Battery Technology: Power the Next Generation of Electronics with Carbon July 23rd, 2014

University of Houston researchers create new method to draw molecules from live cells: Technique using magnetic nanomaterials offers promise for diagnosis, gene therapy July 17th, 2014

Nanomedicine

Arrowhead to Report Fiscal 2014 Third Quarter Financial Results- Conference Call Scheduled for Tuesday, August 12, 2014 - July 31st, 2014

New imaging agent provides better picture of the gut July 30th, 2014

Zenosense, Inc. July 29th, 2014

Optimum inertial design for self-propulsion: A new study investigates the effects of small but finite inertia on the propulsion of micro and nano-scale swimming machines July 29th, 2014

Announcements

Industrial Nanotech, Inc. to Publish PCAOB Audited Financials July 31st, 2014

Nanostructured metal-oxide catalyst efficiently converts CO2 to methanol: Highly reactive sites at interface of 2 nanoscale components could help overcome hurdle of using CO2 as a starting point in producing useful products July 31st, 2014

Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method July 31st, 2014

Pressure probing potential photoelectronic manufacturing compound July 31st, 2014

Nanobiotechnology

Harris & Harris Group Invests in Unique NYC Biotech Accelerator July 29th, 2014

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE