Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Researchers Turn Up Brightness on Fluorescent Probes

Yeast cells labeled with fluoromodules (top) glow brighter (bottom) when researchers incorporate dyedrons into the fluoromodule complex. The fluoromodules are expressed on the cells' surface.
Yeast cells labeled with fluoromodules (top) glow brighter (bottom) when researchers incorporate dyedrons into the fluoromodule complex. The fluoromodules are expressed on the cells' surface.

Abstract:
Development Will Open New Avenues for Research

Researchers Turn Up Brightness on Fluorescent Probes

Pittsburgh, PA | Posted on August 10th, 2010

Researchers from Carnegie Mellon University's Molecular Biosensor and Imaging Center (MBIC) are turning up the brightness on a group of fluorescent probes called fluoromodules that are used to monitor biological activities of individual proteins in real-time. This latest advance enhances their fluormodule technology by causing it to glow an order of magnitude brighter than typical fluorescent proteins. The new fluoromodules are five- to seven-times brighter than enhanced green fluorescent protein (EGFP), a development that will open new avenues for research.

In a paper published online in the Journal of the American Chemical Society, MBIC researchers unveil a new class of dendron-based fluorogenic dyes called "dyedrons," that amplify the signal emitted by their fluoromodules.

"By using concepts borrowed from chemistry, the same concepts used in things like quantum dots and light harvesting solar cells, we were able to create a structure that acts like an antenna, intensifying the fluorescence of the entire fluoromodule," said Marcel Bruchez, associate research professor of chemistry and MBIC program director.

MBIC's fluoromodules are made up of a dye called a fluorogen and a fluorgen-activating protein (FAP). The FAP is genetically expressed in a cell and linked to a protein of interest, where it remains dark until it comes into contact with its associated fluorogen. When the protein and dye bind, the complex emits a fluorescent glow, allowing researchers to easily track the protein on the cell surface and within living cells. Fluoromodules are unique in that they do not need to be washed off for specific labeling, they come in a spectrum of colors, and they are more photostable than other fluorescent proteins.

To make the fluoromodules brighter, the researchers amplified the signal of one of their existing probes. They took one of their standard fluorogens, malachite green, and coupled it with another dye called Cy3 in a complex the researchers called a "dyedron." The dyedron is based on a special type of tree-like structure called a dendron, with one malachite green molecule acting as the trunk and several Cy3 molecules acting as the branches.

The two dyes have overlapping emission and absorption spectra - Cy3 typically emits energy at a wavelength where malachite green absorbs energy - and this overlap allows the dyes to efficiently transfer energy between one another. When the Cy3 dye molecules become excited by a light source, such as a laser, they immediately "donate" their excitation energy to malachite green, boosting the signal being emitted by the malachite green.

Each dyedron is approximately 1-2 nanometers and 3000 g/mol in size. The very bright, but very small, dye particles allow the researchers to expand their live-cell imaging research. Previously, when conducting microscopy experiments using fluorescent proteins, fluoromodules and fluorescent dyes, if researchers wanted to increase the brightness, they would either increase the intensity of the laser used to visualize the proteins or label the protein being studied with numerous copies of the fluorescent tag. Both methods had the potential to alter the biology of the system being studied, either through the more intense energy coming from the laser or the increased weight caused by the multiple tags added to the protein. The new approach provides a single compact protein tag with signal enhancement provided by only modestly enlarging the targeted dye molecule.

The MBIC researchers are currently using fluoromodules to study proteins on the cell surface, and hope to take the technology inside of cells in the near future. Additionally, they will be creating dyedrons for their other existing FAP/dye complexes.

This research was funded by the National Institutes of Health (NIH) as part of the American Reinvestment and Recovery Act. MBIC is one of the NIH's National Technology Centers for Networks and Pathways. For more information, visit: www.mbic.cmu.edu.

####

For more information, please click here

Contacts:
Media Contact:
Jocelyn Duffy
412-268-9982

Copyright © Carnegie Mellon University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Govt.-Legislation/Regulation/Funding/Policy

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

'Electronic skin' could improve early breast cancer detection October 29th, 2014

Possible Futures

Imaging electric charge propagating along microbial nanowires October 20th, 2014

Superconducting circuits, simplified: New circuit design could unlock the power of experimental superconducting computer chips October 18th, 2014

Nanocoatings Market By Product Is Expected To Reach USD 8.17 Billion By 2020: Grand View Research, Inc. October 15th, 2014

Perpetuus Carbon Group Receives Independent Verification of its Production Capacity for Graphenes at 140 Tonnes per Annum: Perpetuus Becomes the First Manufacturer in the Sector to Allow Third Party Audit October 7th, 2014

Academic/Education

SUNY Polytechnic Institute Invites the Public to Attend its Popular Statewide 'NANOvember' Series of Outreach and Educational Events October 23rd, 2014

First Canada Excellence Research Chair gets $10 million from the federal government for oilsands research at the University of Calgary: Federal government announces prestigious research chair to study improving oil production efficiency October 19th, 2014

Raytheon, UMass Lowell open on-campus research institute: Industry leaderís researchers to collaborate with faculty, students to move key technologies forward through first-of-its-kind partnership October 11th, 2014

SUNY Colleges of Nanoscale Science and Engineering and National Institute for Occupational Safety and Health Announce Expanded Partnership October 2nd, 2014

Nanomedicine

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Production of Biocompatible Polymers in Iran October 30th, 2014

Amorphous Coordination Polymer Particles as alternative to classical nanoplatforms for nanomedicine October 30th, 2014

'Electronic skin' could improve early breast cancer detection October 29th, 2014

Sensors

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Tiny carbon nanotube pores make big impact October 29th, 2014

MEMS & Sensors Technology Showcase: Finalists Announced for MEMS Executive Congress US 2014 October 23rd, 2014

Announcements

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Nanobiotechnology

Tiny carbon nanotube pores make big impact October 29th, 2014

Molecular beacons shine light on how cells 'crawl' October 27th, 2014

Breakthrough in molecular electronics paves the way for DNA-based computer circuits in the future: DNA-based programmable circuits could be more sophisticated, cheaper and simpler to make October 27th, 2014

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE