Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Researchers Turn Up Brightness on Fluorescent Probes

Yeast cells labeled with fluoromodules (top) glow brighter (bottom) when researchers incorporate dyedrons into the fluoromodule complex. The fluoromodules are expressed on the cells' surface.
Yeast cells labeled with fluoromodules (top) glow brighter (bottom) when researchers incorporate dyedrons into the fluoromodule complex. The fluoromodules are expressed on the cells' surface.

Abstract:
Development Will Open New Avenues for Research

Researchers Turn Up Brightness on Fluorescent Probes

Pittsburgh, PA | Posted on August 10th, 2010

Researchers from Carnegie Mellon University's Molecular Biosensor and Imaging Center (MBIC) are turning up the brightness on a group of fluorescent probes called fluoromodules that are used to monitor biological activities of individual proteins in real-time. This latest advance enhances their fluormodule technology by causing it to glow an order of magnitude brighter than typical fluorescent proteins. The new fluoromodules are five- to seven-times brighter than enhanced green fluorescent protein (EGFP), a development that will open new avenues for research.

In a paper published online in the Journal of the American Chemical Society, MBIC researchers unveil a new class of dendron-based fluorogenic dyes called "dyedrons," that amplify the signal emitted by their fluoromodules.

"By using concepts borrowed from chemistry, the same concepts used in things like quantum dots and light harvesting solar cells, we were able to create a structure that acts like an antenna, intensifying the fluorescence of the entire fluoromodule," said Marcel Bruchez, associate research professor of chemistry and MBIC program director.

MBIC's fluoromodules are made up of a dye called a fluorogen and a fluorgen-activating protein (FAP). The FAP is genetically expressed in a cell and linked to a protein of interest, where it remains dark until it comes into contact with its associated fluorogen. When the protein and dye bind, the complex emits a fluorescent glow, allowing researchers to easily track the protein on the cell surface and within living cells. Fluoromodules are unique in that they do not need to be washed off for specific labeling, they come in a spectrum of colors, and they are more photostable than other fluorescent proteins.

To make the fluoromodules brighter, the researchers amplified the signal of one of their existing probes. They took one of their standard fluorogens, malachite green, and coupled it with another dye called Cy3 in a complex the researchers called a "dyedron." The dyedron is based on a special type of tree-like structure called a dendron, with one malachite green molecule acting as the trunk and several Cy3 molecules acting as the branches.

The two dyes have overlapping emission and absorption spectra - Cy3 typically emits energy at a wavelength where malachite green absorbs energy - and this overlap allows the dyes to efficiently transfer energy between one another. When the Cy3 dye molecules become excited by a light source, such as a laser, they immediately "donate" their excitation energy to malachite green, boosting the signal being emitted by the malachite green.

Each dyedron is approximately 1-2 nanometers and 3000 g/mol in size. The very bright, but very small, dye particles allow the researchers to expand their live-cell imaging research. Previously, when conducting microscopy experiments using fluorescent proteins, fluoromodules and fluorescent dyes, if researchers wanted to increase the brightness, they would either increase the intensity of the laser used to visualize the proteins or label the protein being studied with numerous copies of the fluorescent tag. Both methods had the potential to alter the biology of the system being studied, either through the more intense energy coming from the laser or the increased weight caused by the multiple tags added to the protein. The new approach provides a single compact protein tag with signal enhancement provided by only modestly enlarging the targeted dye molecule.

The MBIC researchers are currently using fluoromodules to study proteins on the cell surface, and hope to take the technology inside of cells in the near future. Additionally, they will be creating dyedrons for their other existing FAP/dye complexes.

This research was funded by the National Institutes of Health (NIH) as part of the American Reinvestment and Recovery Act. MBIC is one of the NIH's National Technology Centers for Networks and Pathways. For more information, visit: www.mbic.cmu.edu.

####

For more information, please click here

Contacts:
Media Contact:
Jocelyn Duffy
412-268-9982

Copyright © Carnegie Mellon University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

New non-invasive method can detect Alzheimer's disease early: MRI probe technology shows brain toxins in living animals for first time December 22nd, 2014

Piezoelectricity in a 2-D semiconductor: Berkeley Lab researchers discovery of piezoelectricty in molybdenum disulfide holds promise for future MEMS December 22nd, 2014

Quantum physics just got less complicated December 22nd, 2014

Enzyme Biosensor Used for Rapid Measurement of Drug December 22nd, 2014

Govt.-Legislation/Regulation/Funding/Policy

Mysteries of ‘Molecular Machines’ Revealed: Phenix software uses X-ray diffraction spots to produce 3-D image December 22nd, 2014

Piezoelectricity in a 2-D semiconductor: Berkeley Lab researchers discovery of piezoelectricty in molybdenum disulfide holds promise for future MEMS December 22nd, 2014

Atom-thick CCD could capture images: Rice University scientists develop two-dimensional, light-sensitive material December 20th, 2014

Oregon researchers glimpse pathway of sunlight to electricity: Collaboration with Lund University uses modified UO spectroscopy equipment to study 'maze' of connections in photoactive quantum dots December 19th, 2014

Possible Futures

A novel method for identifying the body’s ‘noisiest’ networks November 19th, 2014

Researchers discern the shapes of high-order Brownian motions November 17th, 2014

VDMA Electronics Production Equipment: Growth track for 2014 and 2015 confirmed: Business climate survey shows robust industry sector November 14th, 2014

Open Materials Development Will Be Key for HP's Success in 3D Printing: HP can make a big splash in 3D printing, but it needs to shore up technology claims and avoid the temptation of the razor/razor blade business model in order to flourish November 11th, 2014

Academic/Education

SUNY Poly NanoCollege Faculty Member Selected as American Physical Society Fellow: SUNY Poly Associate Professor of Nanoscience Dr. Vincent LaBella Recognized for Significant Technological Innovations that Enable Interactive Learning December 17th, 2014

Nanomedicine expert joins Rice faculty: Gang Bao combines genetic, nano and imaging techniques to fight disease December 17th, 2014

FEI and Oregon Health & Science University Install a Complete Correlative Microscopy Workflow in Newly Built Collaborative Science Facility December 16th, 2014

Student Nanotechnology Laboratories Network Set Up in Iran December 15th, 2014

Nanomedicine

New non-invasive method can detect Alzheimer's disease early: MRI probe technology shows brain toxins in living animals for first time December 22nd, 2014

Enzyme Biosensor Used for Rapid Measurement of Drug December 22nd, 2014

Creation of 'Rocker' protein opens way for new smart molecules in medicine, other fields December 18th, 2014

Iranian Researchers Produce Electrical Pieces Usable in Human Body December 18th, 2014

Sensors

Piezoelectricity in a 2-D semiconductor: Berkeley Lab researchers discovery of piezoelectricty in molybdenum disulfide holds promise for future MEMS December 22nd, 2014

Enzyme Biosensor Used for Rapid Measurement of Drug December 22nd, 2014

Promising new method for rapidly screening cancer drugs: UMass Amherst researchers invent fast, accurate new nanoparticle-based sensor system December 15th, 2014

Graphene Applied in Production of Recyclable Electrodes December 13th, 2014

Announcements

New non-invasive method can detect Alzheimer's disease early: MRI probe technology shows brain toxins in living animals for first time December 22nd, 2014

Piezoelectricity in a 2-D semiconductor: Berkeley Lab researchers discovery of piezoelectricty in molybdenum disulfide holds promise for future MEMS December 22nd, 2014

Quantum physics just got less complicated December 22nd, 2014

Enzyme Biosensor Used for Rapid Measurement of Drug December 22nd, 2014

Nanobiotechnology

Mysteries of ‘Molecular Machines’ Revealed: Phenix software uses X-ray diffraction spots to produce 3-D image December 22nd, 2014

Scientists trace nanoparticles from plants to caterpillars: Rice University study examines how nanoparticles behave in food chain December 16th, 2014

FEI and Oregon Health & Science University Install a Complete Correlative Microscopy Workflow in Newly Built Collaborative Science Facility December 16th, 2014

UCLA engineers first to detect and measure individual DNA molecules using smartphone microscope December 15th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE