Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Kinked Nanopores Slow DNA Passage for Easier Sequencing

Abstract:
Sandia self-assembly and atomic-layer deposition improve process fivefold

Kinked Nanopores Slow DNA Passage for Easier Sequencing

Albuquerque, NM | Posted on August 2nd, 2010

In an innovation critical to improved DNA sequencing, a markedly slower transmission of DNA through nanopores has been achieved by a team led by Sandia National Laboratories researchers.

Solid-state nanopores sculpted from silicon dioxide are generally straight, tiny tunnels more than a thousand times smaller than the diameter of a human hair. They are used as sensors to detect and characterize DNA, RNA and proteins. But these materials shoot through such holes so rapidly that sequencing the DNA passing through them, for example, is a problem.

In a paper published this week online (July 23) in Nature Materials (hardcopy slated for August, Vol.9, pp. 667-675), a team led by Sandia National Laboratories researchers reports using self-assembly techniques to fabricate equally tiny but kinked nanopores. Combined with atomic-layer deposition to modify the chemical characteristics of the nanopores, the innovations achieve a fivefold slowdown in the voltage-driven translocation speeds critically needed in DNA sequencing. (Translocation involves DNA entering and passing completely through the pores, which are only slightly wider than the DNA itself.)

"By control of pore size, length, shape and composition," says lead researcher Jeff Brinker, "we capture the main functional behaviors of protein pores in our solid-state nanopore system." The importance of a fivefold slowdown in this kind of work, Brinker says, is large.

Also of note is the technique's capability to separate single- and double-stranded DNA in an array format. "There are promising DNA sequencing technologies that require this," says Brinker.

The idea of using synthetic solid-state nanopores as single-molecule sensors for detection and characterization of DNA and its sister materials is currently under intensive investigation by researchers around the world. The thrust was inspired by the exquisite selectivity and flux demonstrated by natural biological channels. Researchers hope to emulate these behaviors by creating more robust synthetic materials more readily integrated into practical devices.

Current scientific procedures align the formation of nominally cylindrical or conical pores at right angles to a membrane surface. These are less capable of significantly slowing the passage of DNA than the kinked nanopores.

"We had a pretty simple idea," Brinker says. "We use the self-assembly approaches we pioneered to make ultrathin membranes with ordered arrays of about 3-nanometer diameter pores. We then further tune the pore size via an atomic-layer deposition process we invented. This allows us to control the pore diameter and surface chemistry at the subnanometer scale. Compared to other solid state nanopores developed to date, our system combines finer control of pore size with the development of a kinked pore pathway. In combination, these allow slowing down the DNA velocity."

The work is supported by the Air Force Office of Scientific Research, the Department of Energy's Basic Energy Sciences and Sandia's Laboratory Directed Research and Development office.

In addition to Brinker, participating team members include Sandians David Adams, Carter Hodges and former Sandia post-doctoral student Yingbing Jiang, with University of New Mexico (UNM) researchers Zhu Chen, Darren Dunphy, Nanguo Liu, and George Xomeritakas. Other research participants are from the UNM School of Pharmacy, the University of Illinois at Urbana-Champaign's Beckman Institute and Mechanical Science and Engineering Dept., and Purdue University's School of Chemical Engineering.

Brinker is a Sandia Fellow, and Distinguished and Regent's Professor of Chemical and Nuclear Engineering and Molecular Genetics and Microbiology at UNM.

The paper is slated to be published hardcopy in August 2010 (Vol.9, pp. 667-675) of Nature Materials.

####

For more information, please click here

Contacts:
Neal Singer

505-845-7078
DOE/Sandia National Laboratories

Copyright © Sandia National Laboratories

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Promising Step Taken in Iran towards Treatment of Spinal Cord Injury August 3rd, 2015

Diagnosis of Salmonella Bacterium-Caused Food Poisoning by Biosensors August 3rd, 2015

Thin films offer promise for ferroelectric devices: Researchers at Tokyo Institute of Technology demystify the ferroelectric properties observed in hafnium-oxide-based thin films, revealing a potentially useful device material August 3rd, 2015

Kalam: versatility personified August 1st, 2015

Govt.-Legislation/Regulation/Funding/Policy

Self-assembling, biomimetic membranes may aid water filtration August 1st, 2015

Kalam: versatility personified August 1st, 2015

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

Theoretical Physicists at Freie Universität Berlin Develop New Insights into Interface between Classical and Quantum Worlds July 31st, 2015

Possible Futures

Nanofiltration Membrane Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Nanozirconia Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Self-Healing Nano Anti-rust Coatings Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Nano Spray Instrument Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Academic/Education

Pakistani Students Who Survived Terror Attack to Attend Weeklong “NanoDiscovery Institute” at SUNY Poly CNSE in Albany July 29th, 2015

Deben reports on the use of their CT500 in the X-ray microtomography laboratory at La Trobe University, Melbourne, Australia July 22nd, 2015

JPK reports on the use of SPM in the Messersmith Group at UC Berkeley looking at biologically inspired polymer adhesives. July 21st, 2015

Renishaw adds Raman analysis to Scanning Electron Microscopy at the University of Sydney, Australia July 9th, 2015

Self Assembly

Self-assembling, biomimetic membranes may aid water filtration August 1st, 2015

New computer model could explain how simple molecules took first step toward life: Two Brookhaven researchers developed theoretical model to explain the origins of self-replicating molecules July 28th, 2015

Spintronics: Molecules stabilizing magnetism: Organic molecules fixing the magnetic orientation of a cobalt surface/ building block for a compact and low-cost storage technology/ publication in Nature Materials July 25th, 2015

Imec introduces self-assembled monomolecular organic films to seal ultra-porous low- k materials: Method prevents leakage of barrier precursors during the interconnect metallization scheme July 15th, 2015

Nanomedicine

Promising Step Taken in Iran towards Treatment of Spinal Cord Injury August 3rd, 2015

Diagnosis of Salmonella Bacterium-Caused Food Poisoning by Biosensors August 3rd, 2015

Gold-diamond nanodevice for hyperlocalised cancer therapy: Gold nanorods can be used as remote controlled nanoheaters delivering the right amount of thermal treatment to cancer cells, thanks to diamond nanocrystals used as temperature sensors August 1st, 2015

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

Announcements

Promising Step Taken in Iran towards Treatment of Spinal Cord Injury August 3rd, 2015

Diagnosis of Salmonella Bacterium-Caused Food Poisoning by Biosensors August 3rd, 2015

Thin films offer promise for ferroelectric devices: Researchers at Tokyo Institute of Technology demystify the ferroelectric properties observed in hafnium-oxide-based thin films, revealing a potentially useful device material August 3rd, 2015

Advances and Applications in Biosensing, Sensor Power, and Sensor R&D to be Covered at Sensors Global Summit August 1st, 2015

Nanobiotechnology

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

European Technology Platform for Nanomedicine and ENATRANS European Consortium Launch the 2nd edition of the Nanomedicine Award: The Award to be presented at BIO-Europe conference in Munich, November 2015 July 30th, 2015

New computer model could explain how simple molecules took first step toward life: Two Brookhaven researchers developed theoretical model to explain the origins of self-replicating molecules July 28th, 2015

Spintronics: Molecules stabilizing magnetism: Organic molecules fixing the magnetic orientation of a cobalt surface/ building block for a compact and low-cost storage technology/ publication in Nature Materials July 25th, 2015

Research partnerships

Newly-Developed Polymers Control Size of Nanoparticles during Production Process July 30th, 2015

Meet the high-performance single-molecule diode: Major milestone in molecular electronics scored by Berkeley Lab and Columbia University team July 29th, 2015

Spintronics: Molecules stabilizing magnetism: Organic molecules fixing the magnetic orientation of a cobalt surface/ building block for a compact and low-cost storage technology/ publication in Nature Materials July 25th, 2015

Stretching the limits on conducting wires July 25th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project