Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Dot Products

Abstract:
Analytical Applications of Enzymatic Growth of Quantum Dots

Dot Products

Weinheim, Germany | Posted on June 16th, 2010

The search for time and cost effective as well as sensitive methods for bioanalytical assays is currently of great interest. At the center of Biofunctional Nanomaterials in San Sebastian (Spain), Valery Pavlov and his co-workers are undertaking research in the area of the design and preparation of new biomaterials for such applications. They report on a new analytical approach in which the enzymatic generation of quantum dots can be applied to the detection of enzymatic activities in Chemistry—A European Journal.

Bioanalytical assays based on the enzymatic generation of nanoparticles are not very common and mostly are limited to biochemical growth of nonfluorescent metal nanoparticles. The main drawback of such systems is that only UV/Vis spectroscopy can be utilized for the optical reading of a signal. Fluorescence spectroscopy, on the other hand, provides an alternative and more sensitive method of analysis.

Valery and and co-workers utilise the fact that alkaline phosphatase (ALP) can induce the formation of H2S. In the presence of cadmium cations, H2S reacts to yield CdS quantum dots that show a characteristic fluorescence, dependent on the concentration of enzyme or substrate. The formation of CdS quantum dots can be measured by fluorescence spectroscopy and represents an alternative to other analytical methods.

ALP is an enzyme that removes phosphate groups from biomolecules, such as alkaloids, proteins and nucleotides and finds wide application in bioanalysis. This enzyme is broadly used as a label in enzyme-linked immunosorbent assays (ELISA). Another important application of ALP is the monitoring of pasteurization in cows' milk—well-pasteurized milk should not demonstrate any phosphatase activity due to enzyme deactivation at elevated temperature. In addition the measurement of ALP activity in human blood serum is used for the diagnostics of viral acute and chronic hepatitis and cirrhosis. The disadvantages of the current methods include high costs, special storage conditions, and maximum fluorescence of the reaction product at alkaline pH. This new assay based on the enzymatic generation of quantum dots and detection by fluorescence spectroscopy represents a step in the direction of finding a method that can significantly reduce time and costs related with these important tests.

Author: Valery Pavlov, CIC biomaGUNE, San Sebastian (Spain), www.cicbiomagune.es/

Title: Analytical Applications of Enzymatic Growth of Quantum Dots

Chemistry - A European Journal 2010, 16, No. 21, 6187-6192, Permalink to the article: dx.doi.org/10.1002/chem.200903373

####

For more information, please click here

Copyright © Chemistry - A European Journal

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Nanocellulose Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

Theoretical Physicists at Freie Universität Berlin Develop New Insights into Interface between Classical and Quantum Worlds July 31st, 2015

Nanomaterials In Cosmetic And Personal Care Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Academic/Education

Pakistani Students Who Survived Terror Attack to Attend Weeklong “NanoDiscovery Institute” at SUNY Poly CNSE in Albany July 29th, 2015

Deben reports on the use of their CT500 in the X-ray microtomography laboratory at La Trobe University, Melbourne, Australia July 22nd, 2015

JPK reports on the use of SPM in the Messersmith Group at UC Berkeley looking at biologically inspired polymer adhesives. July 21st, 2015

Renishaw adds Raman analysis to Scanning Electron Microscopy at the University of Sydney, Australia July 9th, 2015

Nanomedicine

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

European Technology Platform for Nanomedicine and ENATRANS European Consortium Launch the 2nd edition of the Nanomedicine Award: The Award to be presented at BIO-Europe conference in Munich, November 2015 July 30th, 2015

Take a trip through the brain July 30th, 2015

Sol-gel capacitor dielectric offers record-high energy storage July 30th, 2015

Sensors

Controlling Dynamic Behavior of Carbon Nanosheets in Structures Made Possible July 30th, 2015

Non-Enzyme Sensor Determines Level of Blood Sugar July 29th, 2015

American Chemical Society expands reach to include rapidly emerging area of sensor science July 25th, 2015

UT Dallas nanotechnology research leads to super-elastic conducting fibers July 24th, 2015

Announcements

Nanocellulose Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

Theoretical Physicists at Freie Universität Berlin Develop New Insights into Interface between Classical and Quantum Worlds July 31st, 2015

Nanomaterials In Cosmetic And Personal Care Market 2015 - Global Industry Survey, Analysis, Size, Share, Outlook and Forecast to 2020 July 31st, 2015

Quantum Dots/Rods

Quantum networks: Back and forth are not equal distances! July 28th, 2015

Superfast fluorescence sets new speed record: Plasmonic device has speed and efficiency to serve optical computers July 27th, 2015

Engineered hybrid crystal opens new frontiers for high-efficiency lighting: University of Toronto researchers successfully combine 2 different materials to create new hyper-efficient light-emitting crystal July 16th, 2015

Down to the quantum dot: Jülich researchers develop ultrahigh-resolution 3-D microscopy technique for electric fields July 7th, 2015

Nanobiotechnology

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

European Technology Platform for Nanomedicine and ENATRANS European Consortium Launch the 2nd edition of the Nanomedicine Award: The Award to be presented at BIO-Europe conference in Munich, November 2015 July 30th, 2015

New computer model could explain how simple molecules took first step toward life: Two Brookhaven researchers developed theoretical model to explain the origins of self-replicating molecules July 28th, 2015

Spintronics: Molecules stabilizing magnetism: Organic molecules fixing the magnetic orientation of a cobalt surface/ building block for a compact and low-cost storage technology/ publication in Nature Materials July 25th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project