Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Taiwan Scientists Advance Technology Used for Quantitative Measurement of Nano-/microparticle Uptake by Cells

Abstract:
A Taiwan research team has developed a new approach for investigating the amount of nano-/microparticles taken up by mammalian cells.

Taiwan Scientists Advance Technology Used for Quantitative Measurement of Nano-/microparticle Uptake by Cells

Taiwan | Posted on May 31st, 2010

This project was led by Dr. Chung-Hsuan Chen, Distinguished Research Fellow and Director of the Genomics Research Center (GRC) at Academia Sinica, and Dr. Wen-Ping Peng, a Jointly-appointed Assistant Professor of the GRC and the Department of Physics at National Dong Hwa University of Taiwan, in collaboration with Dr. Alice Yu, Distinguished Research Fellow and Deputy Director of the GRC. The research was published online in the leading international journal, Angewandte Chemie, on April 13, 2010.

Nano-/microparticles have been pursued by researchers in recent years as possible carriers for drug delivery. Therefore, effective quantitative measurement of the cellular uptake of nano-/microparticles is of great importance. Conventionally these tiny particles have been measured using certain types of mass spectrometry (an analytical technique for the determination of the elemental composition of a sample or molecule) including Inductively Coupled Plasma Atomic Emission Spectroscopy and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). However, these methods of measurement are limited to elemental particles such as gold nanoparticles.

For this study, scientists used a different type of spectrometer called the Charge-Monitoring Mass Spectrometer (CMS), a device which was itself developed by a group at the GRC and the Institute of Atomic and Molecular Sciences at Academia Sinica in 2007 to measure single cancer cells at higher speeds. This time, researchers from the GRC Physical and Computational Genomics group used this recently-developed device to measure the masses of cells of nano-/microparticle, a novel approach for investigating the amount of nano-/microparticle uptake into mammalian cells.

The group's findings indicate that the CMS is an adequate tool for time-resolved measurements of nanogold uptake by cells. They also found that the CMS could measure particles over a larger size range from 30nm to 250 nm. In addition, as the CMS only takes two-steps to examine the nanogold and the ICP-MS takes five-steps, the CMS analysis is more time-efficient. Most importantly, however, the CMS will also be able to measure non-metal nano particles, including polymeric nanoparticles, carbon nanotubes, diamond nanoparticles, viruses, micelles and liposomes in addition to elemental particles.

"This research brings more efficiency to the measurement and detection of the mass changes of a cell as a result of malignancy or the uptake of nanoparticles. The CMS is proven to be useful in streamlining the determination the quantity of both gold and polystyrene nano-/microparticles taken up into cells. We expect that such device will be of importance in medical applications," noted the first author of the study, Huan-Chang Lin, a Ph.D. candidate in the Department of Bio-Industrial Mechatronics Engineering at National Taiwan University.

This research is co-funded by the National Science Council, Academia Sinica and the National Health Research Institute. The full list of authors is Huan-Chang Lin, Hsin-Hung Lin, Cai-Yu Kao, Alice L. Yu, Wen-Ping Peng and Chung-Hsuan Chen.

The full-text of the study was published under the title Quantitative Measurement of Nano-/Microparticle Endocytosis by Cell Mass Spectrometry, available at the Angewandte Chemie website at: www3.interscience.wiley.com/journal/123349954/abstract?CRETRY=1&SRETRY=0.

Angewandte Chemie is published by the German Chemical Society. In 2008 it had an impact factor of 10.879.

####

For more information, please click here

Contacts:
Media Contacts:
Dr. Chung-Hsuan Chen
Genomics Center, Academia Sinica
(Tel) +886-2-2787-1200

Dr. Wen-Ping Peng
Department of Physics
National Dong Hwa University
(Tel) +886-3-8633-733

Fang-Hsun Yeh
Public Relations Office, Central Office of Administration
Academia Sinica
(Tel) +886-2-2789-8824
(M) 0922-036-691


Mei-Hui Lin
Public Relations Office, Central Office of Administration
Academia Sinica
(Tel) +886-2-2789-8821
(M) 0921-845-234

Copyright © Academia Sinica

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

SEMATECH to Showcase Innovation and Advances in Manufacturing at SEMICON Japan 2014: SEMATECH experts will share the latest techniques, emerging trends and best practices in advanced manufacturing strategies and methodologies November 26th, 2014

Australian startup creates world’s first 100% cotton hydrophobic T-Shirts November 26th, 2014

The mysterious 'action at a distance' between liquid containers November 26th, 2014

'Giant' charge density disturbances discovered in nanomaterials: Juelich researchers amplify Friedel oscillations in thin metallic films November 26th, 2014

Govt.-Legislation/Regulation/Funding/Policy

Lawrence Livermore researchers develop efficient method to produce nanoporous metals November 25th, 2014

Renishaw receives Queen's Award for spectroscopy developments November 25th, 2014

Vegetable oil ingredient key to destroying gastric disease bacteria: In mice, therapeutic nanoparticles dampen H. pylori bacteria and inflammation that lead to ulcers and gastric cancer November 25th, 2014

Research yields material made of single-atom layers that snap together like Legos November 25th, 2014

Academic/Education

SUNY Poly Student Awarded Fellowship with the U.S. Department of Energy's Postgraduate Research Program: Ph.D. Candidate Accepts Postmaster's Appointment To Conduct Research At Albany NanoTech Complex November 13th, 2014

SUNY Polytechnic Institute Hosts Massive Crowd of More Than 3,000 People Who Attended Community Day Activities Across New York State: CNSE’s ‘NANOvember’ kickoff event highlights New York State’s growing high-tech sector with open house events in Albany, Utica, and Rochester November 3rd, 2014

SUNY Polytechnic Institute Invites the Public to Attend its Popular Statewide 'NANOvember' Series of Outreach and Educational Events October 23rd, 2014

First Canada Excellence Research Chair gets $10 million from the federal government for oilsands research at the University of Calgary: Federal government announces prestigious research chair to study improving oil production efficiency October 19th, 2014

Nanomedicine

Vegetable oil ingredient key to destroying gastric disease bacteria: In mice, therapeutic nanoparticles dampen H. pylori bacteria and inflammation that lead to ulcers and gastric cancer November 25th, 2014

Research reveals how our bodies keep unwelcome visitors out of cell nuclei November 24th, 2014

ASU, IBM move ultrafast, low-cost DNA sequencing technology a step closer to reality November 24th, 2014

An Inside Job: UC-Designed Nanoparticles Infiltrate, Kill Cancer Cells From Within November 24th, 2014

Announcements

SEMATECH to Showcase Innovation and Advances in Manufacturing at SEMICON Japan 2014: SEMATECH experts will share the latest techniques, emerging trends and best practices in advanced manufacturing strategies and methodologies November 26th, 2014

Australian startup creates world’s first 100% cotton hydrophobic T-Shirts November 26th, 2014

The mysterious 'action at a distance' between liquid containers November 26th, 2014

'Giant' charge density disturbances discovered in nanomaterials: Juelich researchers amplify Friedel oscillations in thin metallic films November 26th, 2014

Tools

Renishaw receives Queen's Award for spectroscopy developments November 25th, 2014

JPK reports on the use of AFM and the CellHesion module to study plant cells at the University of Queensland November 25th, 2014

A*STAR SIMTech wins international award for breaking new ground in actuators: SIMTech invention can be used in an array of industries, and is critical for next generation ultra-precision systems November 24th, 2014

Professional AFM Images with a Three Step Click SmartScan by Park Systems Revolutionizes Atomic Force Microscopy by Automatizing the Imaging Process November 24th, 2014

Nanobiotechnology

Quantum mechanical calculations reveal the hidden states of enzyme active sites November 20th, 2014

Tokyo Institute of Technology research: Protein-engineered cages aid studies of cell functions November 19th, 2014

A novel method for identifying the body’s ‘noisiest’ networks November 19th, 2014

Implementation of DNA Chains in Designing Nanospin Pieces November 9th, 2014

Research partnerships

SEMATECH to Showcase Innovation and Advances in Manufacturing at SEMICON Japan 2014: SEMATECH experts will share the latest techniques, emerging trends and best practices in advanced manufacturing strategies and methodologies November 26th, 2014

The mysterious 'action at a distance' between liquid containers November 26th, 2014

Lawrence Livermore researchers develop efficient method to produce nanoporous metals November 25th, 2014

Vegetable oil ingredient key to destroying gastric disease bacteria: In mice, therapeutic nanoparticles dampen H. pylori bacteria and inflammation that lead to ulcers and gastric cancer November 25th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More












ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE