Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Taiwan Scientists Advance Technology Used for Quantitative Measurement of Nano-/microparticle Uptake by Cells

Abstract:
A Taiwan research team has developed a new approach for investigating the amount of nano-/microparticles taken up by mammalian cells.

Taiwan Scientists Advance Technology Used for Quantitative Measurement of Nano-/microparticle Uptake by Cells

Taiwan | Posted on May 31st, 2010

This project was led by Dr. Chung-Hsuan Chen, Distinguished Research Fellow and Director of the Genomics Research Center (GRC) at Academia Sinica, and Dr. Wen-Ping Peng, a Jointly-appointed Assistant Professor of the GRC and the Department of Physics at National Dong Hwa University of Taiwan, in collaboration with Dr. Alice Yu, Distinguished Research Fellow and Deputy Director of the GRC. The research was published online in the leading international journal, Angewandte Chemie, on April 13, 2010.

Nano-/microparticles have been pursued by researchers in recent years as possible carriers for drug delivery. Therefore, effective quantitative measurement of the cellular uptake of nano-/microparticles is of great importance. Conventionally these tiny particles have been measured using certain types of mass spectrometry (an analytical technique for the determination of the elemental composition of a sample or molecule) including Inductively Coupled Plasma Atomic Emission Spectroscopy and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). However, these methods of measurement are limited to elemental particles such as gold nanoparticles.

For this study, scientists used a different type of spectrometer called the Charge-Monitoring Mass Spectrometer (CMS), a device which was itself developed by a group at the GRC and the Institute of Atomic and Molecular Sciences at Academia Sinica in 2007 to measure single cancer cells at higher speeds. This time, researchers from the GRC Physical and Computational Genomics group used this recently-developed device to measure the masses of cells of nano-/microparticle, a novel approach for investigating the amount of nano-/microparticle uptake into mammalian cells.

The group's findings indicate that the CMS is an adequate tool for time-resolved measurements of nanogold uptake by cells. They also found that the CMS could measure particles over a larger size range from 30nm to 250 nm. In addition, as the CMS only takes two-steps to examine the nanogold and the ICP-MS takes five-steps, the CMS analysis is more time-efficient. Most importantly, however, the CMS will also be able to measure non-metal nano particles, including polymeric nanoparticles, carbon nanotubes, diamond nanoparticles, viruses, micelles and liposomes in addition to elemental particles.

"This research brings more efficiency to the measurement and detection of the mass changes of a cell as a result of malignancy or the uptake of nanoparticles. The CMS is proven to be useful in streamlining the determination the quantity of both gold and polystyrene nano-/microparticles taken up into cells. We expect that such device will be of importance in medical applications," noted the first author of the study, Huan-Chang Lin, a Ph.D. candidate in the Department of Bio-Industrial Mechatronics Engineering at National Taiwan University.

This research is co-funded by the National Science Council, Academia Sinica and the National Health Research Institute. The full list of authors is Huan-Chang Lin, Hsin-Hung Lin, Cai-Yu Kao, Alice L. Yu, Wen-Ping Peng and Chung-Hsuan Chen.

The full-text of the study was published under the title Quantitative Measurement of Nano-/Microparticle Endocytosis by Cell Mass Spectrometry, available at the Angewandte Chemie website at: www3.interscience.wiley.com/journal/123349954/abstract?CRETRY=1&SRETRY=0.

Angewandte Chemie is published by the German Chemical Society. In 2008 it had an impact factor of 10.879.

####

For more information, please click here

Contacts:
Media Contacts:
Dr. Chung-Hsuan Chen
Genomics Center, Academia Sinica
(Tel) +886-2-2787-1200

Dr. Wen-Ping Peng
Department of Physics
National Dong Hwa University
(Tel) +886-3-8633-733

Fang-Hsun Yeh
Public Relations Office, Central Office of Administration
Academia Sinica
(Tel) +886-2-2789-8824
(M) 0922-036-691


Mei-Hui Lin
Public Relations Office, Central Office of Administration
Academia Sinica
(Tel) +886-2-2789-8821
(M) 0921-845-234

Copyright © Academia Sinica

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Making nanowires from protein and DNA September 3rd, 2015

Making fuel from light: Argonne research sheds light on photosynthesis and creation of solar fuel September 3rd, 2015

Reversible Writing with Light: Self-assembling nanoparticles take their cues from their surroundings September 3rd, 2015

Phagraphene, a 'relative' of graphene, discovered September 2nd, 2015

Govt.-Legislation/Regulation/Funding/Policy

Making nanowires from protein and DNA September 3rd, 2015

Making fuel from light: Argonne research sheds light on photosynthesis and creation of solar fuel September 3rd, 2015

Reversible Writing with Light: Self-assembling nanoparticles take their cues from their surroundings September 3rd, 2015

Silk bio-ink could help advance tissue engineering with 3-D printers September 2nd, 2015

Academic/Education

Sustainable nanotechnology center September 1st, 2015

National Science Foundation Selects SUNY Poly CNSE for Expanded $2.1M Northeast Advanced Technological Education Center: NSF Center Locates to NanoCollege in Support of Flourishing Tech Industry in NYS September 1st, 2015

Announcing Oxford Instruments and School of Physics signing a Memorandum of Understanding August 26th, 2015

Kwansei Gakuin University in Hyogo, Japan, uses Raman microscopy to study crystallographic defects in silicon carbide wafers August 25th, 2015

Nanomedicine

Making nanowires from protein and DNA September 3rd, 2015

Reversible Writing with Light: Self-assembling nanoparticles take their cues from their surroundings September 3rd, 2015

Silk bio-ink could help advance tissue engineering with 3-D printers September 2nd, 2015

Using DNA origami to build nanodevices of the future September 1st, 2015

Announcements

Making nanowires from protein and DNA September 3rd, 2015

Making fuel from light: Argonne research sheds light on photosynthesis and creation of solar fuel September 3rd, 2015

Reversible Writing with Light: Self-assembling nanoparticles take their cues from their surroundings September 3rd, 2015

A marine creature's magic trick explained: Crystal structures on the sea sapphire's back appear differently depending on the angle of reflection September 2nd, 2015

Tools

Oxford Instruments’ Triton Cryofree dilution refrigerator selected by Oxford University for developing scalable quantum nanodevices September 2nd, 2015

JEOL Introduces New Best-in-Class Field Emission SEM September 2nd, 2015

Atomic Force Microscopes from Asylum Research Guide the Development of Thin Film Deposition and Etch Processes September 2nd, 2015

Nanolab Technologies LEAPS Forward with High-Performance Analysis Services to the World: Nanolab Orders Advanced Local Electrode Atom Probe (LEAP®) Microscope from CAMECA Unit of AMETEK Materials Analysis Division August 27th, 2015

Nanobiotechnology

Making nanowires from protein and DNA September 3rd, 2015

A marine creature's magic trick explained: Crystal structures on the sea sapphire's back appear differently depending on the angle of reflection September 2nd, 2015

Using DNA origami to build nanodevices of the future September 1st, 2015

Researchers use DNA 'clews' to shuttle CRISPR-Cas9 gene-editing tool into cells August 30th, 2015

Research partnerships

Turning clothing into information displays September 2nd, 2015

Sustainable nanotechnology center September 1st, 2015

$200K Awarded to Develop In Vitro Lung Test for Toxicity of Inhaled Nanomaterials: In Vitro Lung Test Designed to Protect Human Health and Replace Animal Testing September 1st, 2015

Hot electrons point the way to perfect light absorption: Physicists study how to achieve perfect absorption of light with the help of rough ultrathin films September 1st, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic