Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Taiwan Scientists Advance Technology Used for Quantitative Measurement of Nano-/microparticle Uptake by Cells

Abstract:
A Taiwan research team has developed a new approach for investigating the amount of nano-/microparticles taken up by mammalian cells.

Taiwan Scientists Advance Technology Used for Quantitative Measurement of Nano-/microparticle Uptake by Cells

Taiwan | Posted on May 31st, 2010

This project was led by Dr. Chung-Hsuan Chen, Distinguished Research Fellow and Director of the Genomics Research Center (GRC) at Academia Sinica, and Dr. Wen-Ping Peng, a Jointly-appointed Assistant Professor of the GRC and the Department of Physics at National Dong Hwa University of Taiwan, in collaboration with Dr. Alice Yu, Distinguished Research Fellow and Deputy Director of the GRC. The research was published online in the leading international journal, Angewandte Chemie, on April 13, 2010.

Nano-/microparticles have been pursued by researchers in recent years as possible carriers for drug delivery. Therefore, effective quantitative measurement of the cellular uptake of nano-/microparticles is of great importance. Conventionally these tiny particles have been measured using certain types of mass spectrometry (an analytical technique for the determination of the elemental composition of a sample or molecule) including Inductively Coupled Plasma Atomic Emission Spectroscopy and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). However, these methods of measurement are limited to elemental particles such as gold nanoparticles.

For this study, scientists used a different type of spectrometer called the Charge-Monitoring Mass Spectrometer (CMS), a device which was itself developed by a group at the GRC and the Institute of Atomic and Molecular Sciences at Academia Sinica in 2007 to measure single cancer cells at higher speeds. This time, researchers from the GRC Physical and Computational Genomics group used this recently-developed device to measure the masses of cells of nano-/microparticle, a novel approach for investigating the amount of nano-/microparticle uptake into mammalian cells.

The group's findings indicate that the CMS is an adequate tool for time-resolved measurements of nanogold uptake by cells. They also found that the CMS could measure particles over a larger size range from 30nm to 250 nm. In addition, as the CMS only takes two-steps to examine the nanogold and the ICP-MS takes five-steps, the CMS analysis is more time-efficient. Most importantly, however, the CMS will also be able to measure non-metal nano particles, including polymeric nanoparticles, carbon nanotubes, diamond nanoparticles, viruses, micelles and liposomes in addition to elemental particles.

"This research brings more efficiency to the measurement and detection of the mass changes of a cell as a result of malignancy or the uptake of nanoparticles. The CMS is proven to be useful in streamlining the determination the quantity of both gold and polystyrene nano-/microparticles taken up into cells. We expect that such device will be of importance in medical applications," noted the first author of the study, Huan-Chang Lin, a Ph.D. candidate in the Department of Bio-Industrial Mechatronics Engineering at National Taiwan University.

This research is co-funded by the National Science Council, Academia Sinica and the National Health Research Institute. The full list of authors is Huan-Chang Lin, Hsin-Hung Lin, Cai-Yu Kao, Alice L. Yu, Wen-Ping Peng and Chung-Hsuan Chen.

The full-text of the study was published under the title Quantitative Measurement of Nano-/Microparticle Endocytosis by Cell Mass Spectrometry, available at the Angewandte Chemie website at: www3.interscience.wiley.com/journal/123349954/abstract?CRETRY=1&SRETRY=0.

Angewandte Chemie is published by the German Chemical Society. In 2008 it had an impact factor of 10.879.

####

For more information, please click here

Contacts:
Media Contacts:
Dr. Chung-Hsuan Chen
Genomics Center, Academia Sinica
(Tel) +886-2-2787-1200

Dr. Wen-Ping Peng
Department of Physics
National Dong Hwa University
(Tel) +886-3-8633-733

Fang-Hsun Yeh
Public Relations Office, Central Office of Administration
Academia Sinica
(Tel) +886-2-2789-8824
(M) 0922-036-691


Mei-Hui Lin
Public Relations Office, Central Office of Administration
Academia Sinica
(Tel) +886-2-2789-8821
(M) 0921-845-234

Copyright © Academia Sinica

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Researchers find new way to control light with electric fields May 25th, 2017

Nanometrics Announces Retirement Plans of CEO Timothy Stultz: Dr. Stultz to Continue as Director May 25th, 2017

Nanomechanics, Inc. to Exhibit at the SEM Conference: Nanoindentation experts will attend and exhibit their instruments at the Conference and Exposition on Experimental and Applied Mechanics in Indianapolis May 25th, 2017

Three-dimensional graphene: Experiment at BESSY II shows that optical properties are tuneable May 24th, 2017

Govt.-Legislation/Regulation/Funding/Policy

Researchers find new way to control light with electric fields May 25th, 2017

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Graphene-nanotube hybrid boosts lithium metal batteries: Rice University prototypes store 3 times the energy of lithium-ion batteries May 19th, 2017

Stanford scientists use nanotechnology to boost the performance of key industrial catalyst May 18th, 2017

Academic/Education

MIT Energy Initiative awards 10 seed fund grants for early-stage energy research May 4th, 2017

Bar-Ilan University to set up quantum research center May 1st, 2017

California Research Alliance by BASF establishes more than 25 research projects in three years April 26th, 2017

SUNY Polytechnic Institute Announces Total of 172 Teams Selected to Compete in Solar in Your Community Challenge: Teams from 40 states, plus Washington, DC, 2 Territories, and 4 American Indian Reservations, Will Deploy Solar in Underserved Communities April 20th, 2017

Nanomedicine

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Sensors detect disease markers in breath May 19th, 2017

Oddball enzyme provides easy path to synthetic biomaterials May 17th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

Announcements

Researchers find new way to control light with electric fields May 25th, 2017

Nanometrics Announces Retirement Plans of CEO Timothy Stultz: Dr. Stultz to Continue as Director May 25th, 2017

Nanomechanics, Inc. to Exhibit at the SEM Conference: Nanoindentation experts will attend and exhibit their instruments at the Conference and Exposition on Experimental and Applied Mechanics in Indianapolis May 25th, 2017

Three-dimensional graphene: Experiment at BESSY II shows that optical properties are tuneable May 24th, 2017

Tools

Nanometrics Announces Retirement Plans of CEO Timothy Stultz: Dr. Stultz to Continue as Director May 25th, 2017

Nanomechanics, Inc. to Exhibit at the SEM Conference: Nanoindentation experts will attend and exhibit their instruments at the Conference and Exposition on Experimental and Applied Mechanics in Indianapolis May 25th, 2017

Plasmon-powered upconversion nanocrystals for enhanced bioimaging and polarized emission: Plasmonic gold nanorods brighten lanthanide-doped upconversion superdots for improved multiphoton bioimaging contrast and enable polarization-selective nonlinear emissions for novel nanoscal May 19th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

Nanobiotechnology

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Sensors detect disease markers in breath May 19th, 2017

Oddball enzyme provides easy path to synthetic biomaterials May 17th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

Research partnerships

Three-dimensional graphene: Experiment at BESSY II shows that optical properties are tuneable May 24th, 2017

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Sensors detect disease markers in breath May 19th, 2017

Stanford scientists use nanotechnology to boost the performance of key industrial catalyst May 18th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project