Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Three-Dimensional Cell Culture: Making Cells Feel Right at Home

Abstract:
A team of Houston scientists has unveiled a new technique that uses magnetic nanobeads to levitate cells, allowing them to grow into three-dimensional structures.

Three-Dimensional Cell Culture: Making Cells Feel Right at Home

Bethesda, MD | Posted on April 20th, 2010

This technological leap from the flat Petri dish has the potential for significant impact on cancer research, where recent studies have demonstrated that cancer cells growing in two-dimensional sheets are not the optimal systems for studying potential anticancer agents. In fact, techniques for growing cells in three-dimensional structures could save millions of dollars in drug-testing costs.

Renata Pasqualini and Wadih Arap, of the University of Texas M.D. Anderson Cancer Center, and Thomas Killian, of Rice University, led this study, which was reported in the journal Nature Nanotechnology. Dr. Pasqualini is also a member of the University of Texas Health Science Center at Houston Physical Sciences-Oncology Center, one of 12 Centers funded by the National Cancer Institute to foster the development of innovative ideas and new fields of study based on knowledge of the biological and physical laws and principles that define both normal and tumor systems.

The three-dimensional technique is easy enough for most labs to set up immediately. It uses magnetic nanoparticles to levitate cells while they divide and grow. Compared with cell cultures grown on flat surfaces, the three-dimensional cell cultures tend to form tissues that more closely resemble those inside the body. "There's a big push right now to find ways to grow cells in three-dimensional because the body is three-dimensional, and cultures that more closely resemble native tissue are expected to provide better results for preclinical drug tests," said Dr. Killian. "If you could improve the accuracy of early drug screenings by just 10 percent, it's estimated you could save as much as $100 million per drug." For cancer research, the "invisible scaffold" created by the magnetic field goes beyond its potential for producing cell cultures that are more reminiscent of real tumors, which itself would be an important advance, added Dr. Arap.

To make cells levitate, the research team modified a combination of gold nanoparticles and engineered viral particles called "phage" that was developed in the lab of Drs. Arap and Pasqualini. This targeted "nanoshuttle" can deliver payloads to specific organs or tissues.

"A logical next step for us will be to use this additional magnetic property in targeted ways to explore possible applications in the imaging and treatment of tumors," Dr. Arap said.

In the current study, the researchers added magnetic iron oxide nanoparticles to a gel that contains phage. When cells are added to the gel, the phage causes the particles to be absorbed into cells over a few hours. The gel is then washed away, and the nanoparticle-loaded cells are placed in a Petri dish filled with a liquid that promotes cell growth and division. By placing a coin-sized magnet atop the dish's lid, the researchers found that they could lift the cells off the bottom of the dish, concentrate them, and allow them to grow and divide while they were suspended in the liquid. In a key experiment using glioblastoma cells, the investigators found that cells grown in the three-dimensional medium produced proteins that were similar to those produced by gliobastoma tumors in mice, while cells grown in two dimensions did not show this similarity.

This work, which was supported in part by the National Cancer Institute, is detailed in a paper titled, "Three-dimensional tissue culture based on magnetic cell levitation." Investigators from Nano3D Biosciences, which has licensed this technology for commercial development, also participated in this study. An abstract of this paper is available at the journal's Web site.

####

About NCI Alliance for Nanotechnology in Cancer
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Copyright © NCI Alliance for Nanotechnology in Cancer

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

International research partnership tricks the light fantastic March 2nd, 2015

UC research partnership explores how to best harness solar power March 2nd, 2015

Researchers turn unzipped nanotubes into possible alternative for platinum: Aerogel catalyst shows promise for fuel cells March 2nd, 2015

Important step towards quantum computing: Metals at atomic scale March 2nd, 2015

Govt.-Legislation/Regulation/Funding/Policy

Researchers turn unzipped nanotubes into possible alternative for platinum: Aerogel catalyst shows promise for fuel cells March 2nd, 2015

First detailed microscopy evidence of bacteria at the lower size limit of life: Berkeley Lab research provides comprehensive description of ultra-small bacteria February 28th, 2015

Warming up the world of superconductors: Clusters of aluminum metal atoms become superconductive at surprisingly high temperatures February 25th, 2015

SUNY Poly CNSE Researchers and Corporate Partners to Present Forty Papers at Globally Recognized Lithography Conference: SUNY Poly CNSE Research Group Awarded Both ‘Best Research Paper’ and ‘Best Research Poster’ at SPIE Advanced Lithography 2015 forum February 25th, 2015

Possible Futures

European roadmap for graphene science and technology published February 25th, 2015

Quantum research past, present and future for discussion at AAAS February 16th, 2015

World’s first compact rotary 3D printer-cum-scanner unveiled at AAAS by NTU Singapore start-up: With production funded by crowdsourcing, the first unit will be delivered to the United States in March February 16th, 2015

Nanotechnology Electric Vehicle (EV) Market Analysis Report 2015: According to Radiant Insights, Inc February 13th, 2015

Academic/Education

NanoTecNexus Launches New App for Learning About Nanotechnology—STEM Education Project Spearheaded by Interns February 26th, 2015

SUNY Poly CNSE Researchers and Corporate Partners to Present Forty Papers at Globally Recognized Lithography Conference: SUNY Poly CNSE Research Group Awarded Both ‘Best Research Paper’ and ‘Best Research Poster’ at SPIE Advanced Lithography 2015 forum February 25th, 2015

KIT Increases Commitment in Asia: DAAD Funds Two New Projects: Strategic Partnerships with Chinese Universities and Communi-cation Technologies Network February 22nd, 2015

Minus K Technology Announces Its 2015 Vibration Isolator Educational Giveaway to U.S. Colleges and Universities February 18th, 2015

Nanomedicine

New Hopes for Treatment of Intestine Cancer by Edible Nanodrug March 2nd, 2015

Graphene Shows Promise In Eradication Of Stem Cancer Cells March 1st, 2015

Novel Method to Determine Optical Purity of Drug Components March 1st, 2015

Untangling DNA with a droplet of water, a pipet and a polymer: With the 'rolling droplet technique,' a DNA-injected water droplet rolls like a ball over a platelet, sticking the DNA to the plate surface February 27th, 2015

Announcements

International research partnership tricks the light fantastic March 2nd, 2015

UC research partnership explores how to best harness solar power March 2nd, 2015

Researchers turn unzipped nanotubes into possible alternative for platinum: Aerogel catalyst shows promise for fuel cells March 2nd, 2015

Important step towards quantum computing: Metals at atomic scale March 2nd, 2015

Nanobiotechnology

Untangling DNA with a droplet of water, a pipet and a polymer: With the 'rolling droplet technique,' a DNA-injected water droplet rolls like a ball over a platelet, sticking the DNA to the plate surface February 27th, 2015

Bacteria network for food: Bacteria connect to each other and exchange nutrients February 23rd, 2015

Building tailor-made DNA nanotubes step by step: New, block-by-block assembly method could pave way for applications in opto-electronics, drug delivery February 23rd, 2015

Better batteries inspired by lowly snail shells: Biological molecules can latch onto nanoscale components and lock them into position to make high performing Li-ion battery electrodes, according to new research presented at the 59th annual meeting of the Biophysical Society February 12th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE