Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Rapid, Inexpensive DNA Sequencing Moves Closer to Reality

Abstract:
As efforts such as The Cancer Genome Atlas and others generate vast quantities of information about the genetic makeup of different types of cancer, it is becoming increasingly clear that such information has great potential for determining which anticancer drugs should be used to treat a specific patient. However, realizing that potential will require not only that cancer researchers uncover the links between specific gene changes in a given tumor and that tumor's response to a specific drug therapy, but that technologists develop faster methods of detecting specific mutations that would be economical to use on individual patients.

Rapid, Inexpensive DNA Sequencing Moves Closer to Reality

Bethesda, MD | Posted on February 19th, 2010

A technological breakthrough to address this latter issue may be at hand thanks to recent work conducted by Amit Meller and his colleagues at Boston University. Reporting their work in the journal Nano Letters, these investigators described the use of electrically charged nanopores to detect specific genetic sequences as single DNA molecules pass through the pore. If further development proves successful, this method could yield a new approach to mutation detection that does not involve time-consuming and expensive amplification processes.

The investigators built their sequencing device by using a focused electron beam to drill a 4-5 nanometer diameter hole in a silicon nitride membrane. The membrane is then placed between two small fluid chambers and an electric field is applied across the membrane using a pair of silver/silver chloride electrodes. This applied current causes individual DNA molecules to move through the pore, untwisting and unraveling as they enter the pore.

In order to identify a known genetic sequence, the investigators first treat a DNA sample with specific sequences of a DNA analog known as a peptide nucleic acid, or PNA, that will bind to the proper complementary DNA sequence of interest. When the matched DNA-PNA sequence passes through the pore, it produces a marked change in the electrical current passing between the two electrodes, a change that the investigators demonstrated is easily distinguished from unaltered double-stranded DNA, that is, DNA not duplexed with the PNA probe. The device is capable of analyzing one DNA molecule per second.

This work is detailed in a paper titled "Nanopore Based Sequence Specific Detection of Duplex DNA for Genomic Profiling." An abstract of this paper is available at the journal's Web site.

####

About NCI Alliance for Nanotechnology in Cancer
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Copyright © NCI Alliance for Nanotechnology in Cancer

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Ag/ZnO-Nanorods Schottky diodes based UV-PDs are fabricated and tested May 26th, 2017

New metamaterial-enhanced MRI technique tested on humans May 26th, 2017

Controlling 3-D behavior of biological cells using laser holographic techniques May 26th, 2017

Unveiling the quantum necklace: Researchers simulate quantum necklace-like structures in superfluids May 26th, 2017

Possible Futures

Ag/ZnO-Nanorods Schottky diodes based UV-PDs are fabricated and tested May 26th, 2017

New metamaterial-enhanced MRI technique tested on humans May 26th, 2017

Controlling 3-D behavior of biological cells using laser holographic techniques May 26th, 2017

Unveiling the quantum necklace: Researchers simulate quantum necklace-like structures in superfluids May 26th, 2017

Academic/Education

MIT Energy Initiative awards 10 seed fund grants for early-stage energy research May 4th, 2017

Bar-Ilan University to set up quantum research center May 1st, 2017

California Research Alliance by BASF establishes more than 25 research projects in three years April 26th, 2017

SUNY Polytechnic Institute Announces Total of 172 Teams Selected to Compete in Solar in Your Community Challenge: Teams from 40 states, plus Washington, DC, 2 Territories, and 4 American Indian Reservations, Will Deploy Solar in Underserved Communities April 20th, 2017

Nanomedicine

New metamaterial-enhanced MRI technique tested on humans May 26th, 2017

Controlling 3-D behavior of biological cells using laser holographic techniques May 26th, 2017

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Sensors detect disease markers in breath May 19th, 2017

Announcements

Ag/ZnO-Nanorods Schottky diodes based UV-PDs are fabricated and tested May 26th, 2017

New metamaterial-enhanced MRI technique tested on humans May 26th, 2017

Controlling 3-D behavior of biological cells using laser holographic techniques May 26th, 2017

Unveiling the quantum necklace: Researchers simulate quantum necklace-like structures in superfluids May 26th, 2017

Nanobiotechnology

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Sensors detect disease markers in breath May 19th, 2017

Oddball enzyme provides easy path to synthetic biomaterials May 17th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project