Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Micropatterned material surface controls cell orientation

Abstract:
Cells could be orientated in a controlled way on a micro-patterned surface based upon a delicate material technique, and the orientation could be semi-quantitatively described by some statistical parameters, as suggested by the group of DING from Fudan University, Shanghai, CHINA. The study is reported in Issue 18, Volume 54 (September 2009) of the Chinese Science Bulletin as one of the papers in a special issue about Biomedical Materials in this journal.

Micropatterned material surface controls cell orientation

Shanghai, China | Posted on October 13th, 2009

Cell-material interaction is a very important fundamental topic in natural science, yet is too complex to be revealed without unique research methods. Micropatterning technique, especially photolithography, a widely used technique in microelectronic industry, has recently been employed by material scientists and biologists to generate a surface with cell-adhesion contrast to control cell localization. The present study confirms that cells could be well orientated along a micropattern with cell-adhesive stripes in an adhesion-resistant background.

"While cell orientation on a micropattern is not the first observation, our work distinguishes itself by employing a PEG hydrogel instead of a PEG self assembly monolayer as background, and thus the cell adhesion contrast would be maintained for a long time, which guarantees more convenient and convincing observations," noted the corresponding author Jian-dong DING, director of the Key Laboratory of Molecular Engineering of Polymers of the Chinese Ministry of Education and professor of the Department of Macromolecular Science, Fudan University. "This paper further put forward five statistical parameters which describe cell orientation from different aspects."

In this paper, the authors prepared, by the photolithographic transfer technique, stable gold (Au) micropatterns on PEG hydrogel surfaces with defined cell-resistant (PEG hydrogel) and cell-adhesive (gold microstripes) properties. 3T3 fibroblasts were cultured on Au-microstripe surfaces to observe cell adhesion and orientation. Five statistical parameters were defined and used to describe cell orientation on micropatterns. With the increase of inter-stripe distance, the orientational order parameter, the ratio of long and short axes of a cell, and the occupation fraction of cells on stripes increased gradually, whereas the spreading area of a single cell decreased. The abrupt changes of these four parameters did not happen at the same inter-distance. The adhesion ratio of a cell on Au stripes over cell spreading area did not change monotonically as a function of inter-stripe distance. The combination of the five statistical parameters represented well the cell orientation behaviors semi-quantitatively.

This work was a 973 project of nanoscience and financially supported by the National Basic Research Program, and Professor Ding is the current leader of the 973 project of biomedical nanomaterials. Some early research was also partially supported by Key Project of the Chinese Ministry of Education, and Science and Technology Developing Foundation of Shanghai. The first and second authors, Jian-guo SUN and Jian TANG among the three authors of this paper are PhD students of Professor DING.

The present work is ready to be extended by developing pertinent micropatterning technique in three dimensions in Material Science, examination of various kinds of cells in Biology, exploring the underling mechanism of signal transduction in Cell Biology and Molecular Biology, application of the research in Regenerative Medicine such as Tissue Engineering which is related to various surgeries.

Reference: Jianguo Sun, Jian Tang, Jiandong Ding*. Cell orientation on a stripe-micropatterned surface. Chn. Sci. Bull. 2009; 54(18): 3154-3159. www.wjgnet.com/1007-9327/13/4873.asp

####

For more information, please click here

Contacts:
Jiandong DING

0086-216-564-3506

Copyright © Science in China Press

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Graphene and Amaranthus Superparamagnets: Breakthrough nanoparticles discovery of Indian researcher September 23rd, 2014

New NIH/DOE Grant for Life Science Studies at NSLS-II: Funding will support operation of three powerful experimental stations designed to reveal detailed structures of proteins, viruses, and more September 23rd, 2014

BSA Distinguished Lecture Tuesday, 10/14: 'LCLS: A Stunning New View Through X-ray Laser Eyes' September 23rd, 2014

Brookhaven Lab's National Synchrotron Light Source II Approved to Start Routine Operations: Milestone marks transition to exciting new chapter September 23rd, 2014

Nanomedicine

Graphene and Amaranthus Superparamagnets: Breakthrough nanoparticles discovery of Indian researcher September 23rd, 2014

New NIH/DOE Grant for Life Science Studies at NSLS-II: Funding will support operation of three powerful experimental stations designed to reveal detailed structures of proteins, viruses, and more September 23rd, 2014

Nanotubes help healing hearts keep the beat: Rice University, Texas Children’s Hospital patch for defects enhances electrical connections between cells September 23rd, 2014

Immune system is key ally in cyberwar against cancer: Rice University study yields new two-step strategy for weakening cancer September 23rd, 2014

Discoveries

Graphene and Amaranthus Superparamagnets: Breakthrough nanoparticles discovery of Indian researcher September 23rd, 2014

Southampton scientists grow a new challenger to graphene September 23rd, 2014

Future flexible electronics based on carbon nanotubes: Study in Applied Physics Letters show how to improve nanotube transistor and circuit performance with fluoropolymers September 23rd, 2014

Nanotubes help healing hearts keep the beat: Rice University, Texas Children’s Hospital patch for defects enhances electrical connections between cells September 23rd, 2014

Announcements

Future flexible electronics based on carbon nanotubes: Study in Applied Physics Letters show how to improve nanotube transistor and circuit performance with fluoropolymers September 23rd, 2014

Nanotubes help healing hearts keep the beat: Rice University, Texas Children’s Hospital patch for defects enhances electrical connections between cells September 23rd, 2014

Immune system is key ally in cyberwar against cancer: Rice University study yields new two-step strategy for weakening cancer September 23rd, 2014

Los Alamos Researchers Uncover New Properties in Nanocomposite Oxide Ceramics for Reactor Fuel, Fast-Ion Conductors: Misfit dislocations are key to transport properties across material interfaces September 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE