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Home > News > Large Stokes-Shift Fluorescent Silica Nanoparticles with Enhanced Emission Over Free Dye for Single Excitation Multiplexing

September 11th, 2009

Large Stokes-Shift Fluorescent Silica Nanoparticles with Enhanced Emission Over Free Dye for Single Excitation Multiplexing

Abstract:
We describe a polycondensation reaction of silica precursors in a modified Stöber-type basic ethanol solution to produce graded Stokes-shift core-shell silica nanoparticles providing bright and spectrally multiplexed sets of fluorescent tags that are excitable using a single excitation source. Dynamic light scattering and scanning electron microscopy demonstrate particle sizes in the sub-10 nm regime. Absorption matched emission comparisons between encapsulated and free dyes in aqueous solution reveal about an order of magnitude per-dye brightness enhancements that are apparent by simply looking at the solutions under laser illumination conditions.

Source:
interscience.wiley.com

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