- About Us
- Career Center
- Nano-Social Network
- Nano Consulting
- My Account
By combining a gold nanoparticle with a unique family of nucleic acids, researchers at Northwestern University have created a new type of intracellular reporting system that with a flash of light reveals the presence and quantity of a wide variety of biologically important molecules. These so-called nanoflares could provide cancer biologists with a highly sensitive method of tracking complex biochemical processes in real time without interfering with those processes.
Chad A. Mirkin, Ph.D., principal investigator of the Nanomaterials for Cancer Diagnostics and Therapeutics at the Northwestern University Center of Cancer Nanotechnology Excellence, and his colleagues demonstrated the utility of their nanoflares by developing a real-time assay for intracellular adenosine triphosphate (ATP), one of the key energy sources of cellular metabolism. Current methods for ATP analysis require that a cell be destroyed and provide only an average measurement of ATP levels from large number of cells rather than time- and cell-specific measurements. The researchers reported their findings in the journal Nano Letters.
At the center of the nanoflare is a gold nanoparticle coated with a dense layer of nucleic acid aptamers. Aptamers, which are synthesized in the lab, are molecules designed to mimic antibodies in that they bind tightly to a specific chosen molecule. In this case, the aptamers were designed to bind to ATP as well as to the surface of gold nanoparticles. These aptamers were also equipped with a reporter molecule that is capable of producing a bright fluorescent signal.
The key to the nanoflare's unique signaling ability lies in the fact that gold nanoparticles will quench, or prevent, the reporter molecule from emitting its light signal when the attached aptamer is stuck to the nanoparticle. However, when ATP is present, it causes the aptamer to change shape, releasing it from the nanoparticle and allowing the reporter molecule to fluoresce. The amount of aptamer released from the nanoparticle, and hence the intensity of the fluorescent signal, is directly proportional to the amount of ATP present in a cell.
Cells growing in culture rapidly take up the aptamer-coated nanoparticle and soon begin to fluoresce brightly. Then, when the cells are treated with a drug combination known to cause a cell to use up its ATP stores, the fluorescence begins dimming in a dose-dependent manner. Thanks to well-established methods for developing aptamers that will bind to specific biomolecules, it is likely that nanoflares will become a versatile new tool for use in a variety of intracellular processes.
About National Cancer Institute
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.
The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.
Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.
For more information, please click here
National Cancer Institute
Office of Technology & Industrial Relations
ATTN: NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A49
31 Center Drive , MSC 2580
Bethesda , MD 20892-2580
Copyright © National Cancer InstituteIf you have a comment, please Contact us.
Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.
|Related News Press|
News and information
Nanofur for oil spill cleanup: Materials researchers learn from aquatic ferns: Hairy plant leaves are highly oil-absorbing / publication in bioinspiration & biomimetics / video on absorption capacity August 25th, 2016
New theory could lead to new generation of energy friendly optoelectronics: Researchers at Queen's University Belfast and ETH Zurich, Switzerland, have created a new theoretical framework which could help physicists and device engineers design better optoelectronics August 23rd, 2016
50 years after the release of the film 'Fantastic Voyage,' science upstages fiction: Science upstages fiction with nanorobotic agents designed to travel in the human body to treat cancer August 25th, 2016
Nanoparticles that speed blood clotting may someday save lives August 23rd, 2016
Stretchy supercapacitors power wearable electronics August 25th, 2016
Semblant to Present at China Mobile Manufacturing Forum 2016 August 25th, 2016