Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Nanion and Axiogenesis present parallel patch clamp recordings of action potentials from Cor.At cardiomyocytes

Nanion's Patchliner has been used for recordings of action potentials from Axiogenesis Cor.At cardiomyocytes
Nanion's Patchliner has been used for recordings of action potentials from Axiogenesis Cor.At cardiomyocytes

Abstract:
Stem cell derived cardiomyocytes were analyzed using Nanion's automated patch clamp systems, the Patchliner and the Port-a-Patch to validate ion channel composition, the presence of action potentials and the effect of compounds on cardiac ion channels.

Nanion and Axiogenesis present parallel patch clamp recordings of action potentials from Cor.At cardiomyocytes

Germany | Posted on June 23rd, 2009

Stem cell derived cardiomyocytes have tremendous potential for cardiac safety testing of drug candidates. By offering an authentic cellular environment they are closer to the actual physiological situation compared to cell lines with over-expressed ion channels. Axiogenesis is a provider of pure mouse embryonic stem (ES) cell derived cardiomyocytes (Cor.At®), which now have been evaluated with Nanion's automated patch clamp platforms, the Port-a-Patch and the Patchliner. Expected currents were obtained (K, Ca, Na) under voltage clamp conditions, as well as action potentials recorded in current clamp mode. Effects on action potentials of compounds with known effect on cardiac channels were successfully investigated using Nanion's platforms.

Ralf Kettenhofen, Senior Scientist at Axiogenesis, Cologne, Germany, says:
"Nanion's enabling products allow for a wide range of experiments including current clamp recordings of action potentials from our Cor.At® cardiomyocytes. The possibility for not only high quality voltage clamp recordings, but also action potentials is a truly unique feature. Such recordings reflect the total effect of drug action on the cardiac ion channel ensemble rather than effects on individual ion channel species. This is important for investigations of compounds exhibiting chronotropic or arrhythmic effects by either direct action on ion channels or humoral regulation of eg. G-protein coupled receptor agonists."

Niels Fertig, CEO of Nanion Technologies continues:
"The Cor.At® ES cell derived cardiomyocytes in combination with voltage and current clamp recordings provide physiologically relevant information about cardiac drug safety. Our automated patch clamp platforms are known for their flexibility and versatility, which is reflected by the combined voltage- and current clamp measurements used in this study."

The Patchliner and the Port-a-Patch also support temperature control which will be used in further studies of the Cor.At® mouse ES cell derived cardiomyocytes. The Port-a-Patch and the Patchliner are the only automated patch clamp platforms on the market supporting current clamp recordings.
Axiogenesis will present more information about the obtained results at the Ion Channel Retreat Meeting in Vancouver, Canada, June 29th - July 1st.

####

About Nanion
Nanion Technologies GmbH is a German Private Limited Company and was founded in 2002 as a spin-off from the Center for Nanoscience (CeNS) of the University of Munich. Nanion’s team has developed and globally established two highly successful automated patch clamp instruments (Port-a-Patch and Patchliner) as enabling tools for sophisticated and high throughput applications in ion channel research and drug discovery. This year Nanion is introducing their its system, the SyncroPatch 96, a high quality, HTS patch clamp platform.

Nanion's instruments use planar patch clamp chips which replace the traditional glass pipette used in the technique of patch clamping. Nanion was nominated in 2007 for Germany's most prestigious innovation award the Deutscher Zukunftspreis (German Future Prize, Federal President's Award for Technology and Innovation).

About Axiogenesis
Axiogenesis is an innovative and productive biotechnology company located in Cologne, Germany. It was founded in 2000 and was listed on the German stock exchange in 2007. The primary focus of Axiogenesis is the generation and production of pure in vitro differentiated cells that display normal physiological properties. Axiogenesis can either use murine embryonic stem cells (mESC) or human induced pluripotent stem cells (hiPSC) as a source. These cells are then used to develop novel assays for pharmacology and safety screening in the pharmaceutical, chemical, and cosmetics industries.

Pure mouse ES-derived cardiomyocytes (CorAt®) are available in unlimited amounts, can be shipped frozen overnight, and are quality controlled showing no lot-to-lot variation. Protocols and applications include but are not limited to cardiac specific cytotoxicity, electrophysiology, GPCR functionality, siRNA transfection and hypertrophy induction. The cells express and use all essential ion channels and K, Na, and Ca currents have been recorded in patch clamp experiments. In addition, the expected effects of known ion channel modulators and cardio toxic substances are observed. Measurements have been validated on most instruments available.

Using its technology Axiogenesis has consequently designed a Clinical Modeling (CM) platform that is able to accelerate drug development. Axiogenesis is further offering exclusive customization services of cell lines and in house production of cardiomyocytes, endothelial cells, hepatocytes and neuronal cells.

For more information, please click here

Contacts:
Niels Fertig
CEO
Phone: +49 89 2189 97972


Press contact:
Dr. Cecilia Farre
Marketing Director
Nanion Technologies
Nanion Technologies GmbH
Erzgiessereistrasse 4
80335 Munich
Germany

Rudolf Klinghammer
Phone: +49 (221) 9988 1824

Web: www.axiogenesis.com

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Nanoparticles present sustainable way to grow food crops May 1st, 2016

Searching for a nanotech self-organizing principle May 1st, 2016

Clay nanotube-biopolymer composite scaffolds for tissue engineering May 1st, 2016

Cooling graphene-based film close to pilot-scale production April 30th, 2016

Nanomedicine

Clay nanotube-biopolymer composite scaffolds for tissue engineering May 1st, 2016

Nanoparticles hold promise as double-edged sword against genital herpes April 28th, 2016

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

Announcements

Nanoparticles present sustainable way to grow food crops May 1st, 2016

Clay nanotube-biopolymer composite scaffolds for tissue engineering May 1st, 2016

Cooling graphene-based film close to pilot-scale production April 30th, 2016

Personal cooling units on the horizon April 29th, 2016

Events/Classes

Researchers create a first frequency comb of time-bin entangled qubits: Discovery is a significant step toward multi-channel quantum communication and higher capacity quantum computers April 28th, 2016

Introducing the RE標ORK Bio-inspired Robotics Summit in Berlin April 27th, 2016

ORIG3N Added to Companies Presenting at Harris & Harris Group's Annual Meeting, Tuesday June 7, 2016, the New York Genome Center April 27th, 2016

Team builds first quantum cascade laser on silicon: Eliminates the need for an external light source for mid-infrared silicon photonic devices or photonic circuits April 21st, 2016

Alliances/Trade associations/Partnerships/Distributorships

Electrically Conductive Graphene Ink Enables Printing of Biosensors April 23rd, 2016

Leti Extends Collaboration with Qualcomm on CoolCubeTM 3D Integration Technology for High-Density, High-Performance ICs: Collaboration Goals Include Building an Ecosystem To Take the Chip-stacking Technology from Design to Fabrication April 13th, 2016

FEI Partners with Five Pharmaceutical Companies, the Medical Research Council and the University of Cambridge to form Cryo-EM Research Consortium April 5th, 2016

Strem Chemicals and SONA Nanotech Sign Distribution Agreement for the World’s First Gold Nanorods Synthesized without CTAB February 24th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic