Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Nanion and Axiogenesis present parallel patch clamp recordings of action potentials from Cor.At cardiomyocytes

Nanion's Patchliner has been used for recordings of action potentials from Axiogenesis Cor.At cardiomyocytes
Nanion's Patchliner has been used for recordings of action potentials from Axiogenesis Cor.At cardiomyocytes

Abstract:
Stem cell derived cardiomyocytes were analyzed using Nanion's automated patch clamp systems, the Patchliner and the Port-a-Patch to validate ion channel composition, the presence of action potentials and the effect of compounds on cardiac ion channels.

Nanion and Axiogenesis present parallel patch clamp recordings of action potentials from Cor.At cardiomyocytes

Germany | Posted on June 23rd, 2009

Stem cell derived cardiomyocytes have tremendous potential for cardiac safety testing of drug candidates. By offering an authentic cellular environment they are closer to the actual physiological situation compared to cell lines with over-expressed ion channels. Axiogenesis is a provider of pure mouse embryonic stem (ES) cell derived cardiomyocytes (Cor.At®), which now have been evaluated with Nanion's automated patch clamp platforms, the Port-a-Patch and the Patchliner. Expected currents were obtained (K, Ca, Na) under voltage clamp conditions, as well as action potentials recorded in current clamp mode. Effects on action potentials of compounds with known effect on cardiac channels were successfully investigated using Nanion's platforms.

Ralf Kettenhofen, Senior Scientist at Axiogenesis, Cologne, Germany, says:
"Nanion's enabling products allow for a wide range of experiments including current clamp recordings of action potentials from our Cor.At® cardiomyocytes. The possibility for not only high quality voltage clamp recordings, but also action potentials is a truly unique feature. Such recordings reflect the total effect of drug action on the cardiac ion channel ensemble rather than effects on individual ion channel species. This is important for investigations of compounds exhibiting chronotropic or arrhythmic effects by either direct action on ion channels or humoral regulation of eg. G-protein coupled receptor agonists."

Niels Fertig, CEO of Nanion Technologies continues:
"The Cor.At® ES cell derived cardiomyocytes in combination with voltage and current clamp recordings provide physiologically relevant information about cardiac drug safety. Our automated patch clamp platforms are known for their flexibility and versatility, which is reflected by the combined voltage- and current clamp measurements used in this study."

The Patchliner and the Port-a-Patch also support temperature control which will be used in further studies of the Cor.At® mouse ES cell derived cardiomyocytes. The Port-a-Patch and the Patchliner are the only automated patch clamp platforms on the market supporting current clamp recordings.
Axiogenesis will present more information about the obtained results at the Ion Channel Retreat Meeting in Vancouver, Canada, June 29th - July 1st.

####

About Nanion
Nanion Technologies GmbH is a German Private Limited Company and was founded in 2002 as a spin-off from the Center for Nanoscience (CeNS) of the University of Munich. Nanion’s team has developed and globally established two highly successful automated patch clamp instruments (Port-a-Patch and Patchliner) as enabling tools for sophisticated and high throughput applications in ion channel research and drug discovery. This year Nanion is introducing their its system, the SyncroPatch 96, a high quality, HTS patch clamp platform.

Nanion's instruments use planar patch clamp chips which replace the traditional glass pipette used in the technique of patch clamping. Nanion was nominated in 2007 for Germany's most prestigious innovation award the Deutscher Zukunftspreis (German Future Prize, Federal President's Award for Technology and Innovation).

About Axiogenesis
Axiogenesis is an innovative and productive biotechnology company located in Cologne, Germany. It was founded in 2000 and was listed on the German stock exchange in 2007. The primary focus of Axiogenesis is the generation and production of pure in vitro differentiated cells that display normal physiological properties. Axiogenesis can either use murine embryonic stem cells (mESC) or human induced pluripotent stem cells (hiPSC) as a source. These cells are then used to develop novel assays for pharmacology and safety screening in the pharmaceutical, chemical, and cosmetics industries.

Pure mouse ES-derived cardiomyocytes (CorAt®) are available in unlimited amounts, can be shipped frozen overnight, and are quality controlled showing no lot-to-lot variation. Protocols and applications include but are not limited to cardiac specific cytotoxicity, electrophysiology, GPCR functionality, siRNA transfection and hypertrophy induction. The cells express and use all essential ion channels and K, Na, and Ca currents have been recorded in patch clamp experiments. In addition, the expected effects of known ion channel modulators and cardio toxic substances are observed. Measurements have been validated on most instruments available.

Using its technology Axiogenesis has consequently designed a Clinical Modeling (CM) platform that is able to accelerate drug development. Axiogenesis is further offering exclusive customization services of cell lines and in house production of cardiomyocytes, endothelial cells, hepatocytes and neuronal cells.

For more information, please click here

Contacts:
Niels Fertig
CEO
Phone: +49 89 2189 97972


Press contact:
Dr. Cecilia Farre
Marketing Director
Nanion Technologies
Nanion Technologies GmbH
Erzgiessereistrasse 4
80335 Munich
Germany

Rudolf Klinghammer
Phone: +49 (221) 9988 1824

Web: www.axiogenesis.com

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Graphene key to growing 2-dimensional semiconductor with extraordinary properties August 30th, 2016

University of Akron researchers find thin layers of water can become ice-like at room temperature: Results could lead to an assortment of anti-friction solutions August 30th, 2016

Nanocatalysis for organic chemistry: This research article by Dr. Qien Xu et al. is published in Current Organic Chemistry, Volume 20, Issue 19, 2016 August 30th, 2016

Continuous roll-process technology for transferring and packaging flexible LSI August 29th, 2016

Nanomedicine

Designing ultrasound tools with Lego-like proteins August 29th, 2016

Nanofiber scaffolds demonstrate new features in the behavior of stem and cancer cells August 25th, 2016

Johns Hopkins scientists track metabolic pathways to find drug combination for pancreatic cancer August 25th, 2016

50 years after the release of the film 'Fantastic Voyage,' science upstages fiction: Science upstages fiction with nanorobotic agents designed to travel in the human body to treat cancer August 25th, 2016

Announcements

Graphene key to growing 2-dimensional semiconductor with extraordinary properties August 30th, 2016

University of Akron researchers find thin layers of water can become ice-like at room temperature: Results could lead to an assortment of anti-friction solutions August 30th, 2016

Nanocatalysis for organic chemistry: This research article by Dr. Qien Xu et al. is published in Current Organic Chemistry, Volume 20, Issue 19, 2016 August 30th, 2016

Meteorite impact on a nano scale August 29th, 2016

Events/Classes

Stretchy supercapacitors power wearable electronics August 25th, 2016

Semblant to Present at China Mobile Manufacturing Forum 2016 August 25th, 2016

Nanoparticles that speed blood clotting may someday save lives August 23rd, 2016

Impressive List of Doctors, Scientists Coming to Vail for Scientific Summit: The Second Vail Scientific Summit Convenes the Greatest Minds in Regenerative Medicine and Science August 17th, 2016

Alliances/Trade associations/Partnerships/Distributorships

Thomas Swan and NGI announce unique partnership July 28th, 2016

Starpharma initiates new DEP™ drug delivery program with AstraZeneca July 27th, 2016

XEI Scientific Partners with Electron Microscopy Sciences to Promote and Sell its Products in North and South America July 25th, 2016

Leti and Korea Institute of Science and Technology to Explore Collaboration on Advanced Technologies for Digital Era July 14th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic