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Home > Press > A breakthrough toward industrial production of fluorescent nanodiamonds

Abstract:
Activité of Normal & Pathologic Biomolecules- SANPB », Inserm / UEVE U829 (Genopole Evry, France) in collaboration with the Material Centre of Mines-ParisTech (Evry, France), the NRG - UMR 5060 CNRS / UTBM (Technology University of Belfort-Montbéliard) and the Physic Institute of Stuttgart University (Germany) discovered a novel route to fabricate fluorescent nanoparticles from diamond microcrystals. Results are published in Nanotechnology June10 2009 issue.

A breakthrough toward industrial production of fluorescent nanodiamonds

Genopole Evry, France | Posted on June 4th, 2009

Fluorescence is a major tool in life and material sciences. In biology/medicine, the coupling of fluorescent dyes, to proteins or nucleic acids (RNA, DNA) allows one to investigate their fate and interactions in cultured cells or in the body. Similarly, fluorescence is used in material sciences to detect electromagnetic fields, for optic storage or tracking (notably to detect fake products). However, most of fluorescent dyes are made of molecules with a limited life time due to chemical reactivity.

In this context fluorescent diamond nanoparticles present a valuable alternative thanks to their outstanding photophysical properties. They are very bright and possess long-term non-bleaching, non-blinking fluorescence in the red/NIR region. Based on these unique properties, multiple applications are foreseen in physics, material science, biochemistry and biology. However, until recently, the production of such nanoparticles was limited to the laboratory.

A single route is nowadays taken to fabricate such fluorescent nanoparticles. It consists of irradiating substitutional nitrogen-containing diamond nanocrystals, produced by the diamond industry, with electron or ion beams to create vacancies in the crystal lattice. Isolated substitutional nitrogen atoms then trap a moving vacancy during annealing to form a fluorescent NV centre. Unfortunately, the efficiency and yield of this route are low due to amorphization and the loss of moving vacancies to the surface during irradiation and annealing.

A top-down processing of diamond microcrystals, which are less prone to amorphization and vacancy loss, would provide a more industrially scalable route. However, in this case two barriers have to be surmounted - the difficulties of irradiating large amounts of material and converting microdiamonds into nanocrystals while keeping both fluorescence properties and crystal structure intact.

In a recent study, which is published in Nanotechnology, researchers in France and Germany have explored with success this alternative route to producing homogeneous samples of pure and very small fluorescent diamond nanoparticles with high yield. The fabrication procedure starts with the irradiation of finely controlled micron-size diamonds and requires subsequent milling and purification steps. In this novel process, substitutional nitrogen-containing microdiamonds with defined atomic composition were irradiated using a high-energy electron beam and then annealed at high temperature (800 °C) to create the desired photoluminescent centres in an intact diamond lattice. An original two-step milling protocol was designed to convert the fluorescent microdiamond into very small (down to 4 nm) round-shape nanoparticles of highly pure sp3 diamond with very bright and stable photoluminescent centres.

Such a fine fabrication process can now be used for the large-scale production of fluorescent diamond nanoparticles. One can vary and tailor their properties via the composition of the starting material to answer the needs of future applications. These fluorescent diamond nanoparticles open realistic perspectives to very long term labeling, to quantitative biology and innovative nanotechnology applications in composites, optoelectronics or analytical chemistry.

Reference : « High yield fabrication of fluorescent nanodiamonds », Jean-Paul Boudou, Patrick A. Curmi, Fedor Jelezko, Joerg Wrachtrup, Pascal Aubert, Mohamed Sennour, Gopalakrischnan Balasubramanian, Rolf Reuter, Alain Thorel and Eric Gaffet, 2009, Nanotechnology 20 235602

(www.iop.org/EJ/abstract/0957-4484/20/23/235602/)

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For more information, please click here

Contacts:
Patrick Curmi

33-144-236-086

Copyright © INSERM (Institut national de la santé et de la recherche méd

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