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May 22nd, 2007
Evaluating multiple biomarkers with quantum dots
Quantum dots linked to biological molecules, such as antibodies, have shown promise as a new tool for detecting and quantifying a wide variety of cancer-associated molecules. Now, thanks to detailed studies of how to make these labeled quantum dots and use them to detect disease markers, so-called bioconjugated quantum dots may finally be ready for widespread use in the clinic.
Reporting its work in the journal Nature Protocols ("Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry"), a team of investigators at the Emory-Georgia Tech Nanotechnology Center for Personalized and Predictive Oncology provide detailed protocols for linking biomolecules to quantum dots and then using these constructs to detect multiple biomarkers simultaneously. The team, led by Shuming Nie, Ph.D., co-principal investigator at the Emory-Georgia Tech Center, and May Wang, Ph.D., director of biocomputing and bioinformatics at this Center for Cancer Nanotechnology Excellence, also describes the exact methods used to prepare tissue samples to obtain optimal results using the bioconjugated quantum dots.
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