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May 9th, 2007

Florescent microscopy and vibrational spectroscopy combined

Abstract:
Cellular imaging possibilities broadened through combination of fluorescent and Raman microscopy as optical properties of fluorescent ‘quantum dot' nanoparticles are used to replace existing fluorescent labels.

The ‘quantum dot' nanoparticles used by Henk-Jan van Manen and Cees Otto from the Biophysical Engineering Group of the MESA+ Institute for Nanotechnology, replace existing fluorescent labels that are employed to enable the cell's biomolecules to light up under the microscope.

While fluorescence microscopy continues to be instrumental in unravelling the intricate biological processes that take place inside living cells, it would be even more informative to combine it with the intracellular chemical analysis capabilities of vibrational spectroscopy techniques such as Raman microscopy.

Source:
scientistlive.com

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