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February 26th, 2007
Holography was invented in 1947 by Dennis Gabor who received Nobel Prize in 1971 for his work where as before microscopy affected only image variations of intensity as with a photograph. Gabor invention made it possible to record not only intensity but also light phase.
In conventional microscope the object is lead by a conventional light source and enlarged by a lens system. The image shows only contrast in intensity, no accurate 3D or vertical measurement is possible.
In holographic microscopy the beam from a coherent light source or laser is split into two beams. One illuminating the object itself and the other providing a reference beam which when recombine with the other beam creates a phase interference that is registered on the hologram plate.
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