Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Light-induced nanocarriers switch on improved gene therapy

A newly developed, light-induced nanocarrier for targeted gene therapy has been trialled by researchers in Japan. The three-layered micelle safely and effectively delivered gene packages in mice cancer tumor models.
A newly developed, light-induced nanocarrier for targeted gene therapy has been trialled by researchers in Japan. The three-layered micelle safely and effectively delivered gene packages in mice cancer tumor models.

Abstract:
Nanocarriers are a useful means of transporting molecules such as genes to specific points in the body, for example to target tumors in cancer therapy. Such nanocarriers must be carefully controlled to avoid the random dissemination of genes in other organs en route to the target cells.

Light-induced nanocarriers switch on improved gene therapy

Kawasaki City, Japan | Posted on August 5th, 2014

Using light as a ‘switch' to stimulate a nanocarrier into releasing a gene package exactly where it is needed is a potentially safe and effective way of controlling delivery in the body. Now, Nobuhiro Nishiyama at Tokyo Institute of Technology, and Kazunori Kataoka and co-workers at the University of Tokyo, together with scientists across Japan, have designed and built a nanocarrier capable of light-mediated gene delivery into a solid cancerous tumor.

One challenge the researchers faced was the need to compartmentalize the components of the nanocarrier. Photosensitive materials such as dendrimeric photosensitizer (DPc) can trigger photochemical damage to the gene package if they come into direct contact with the plasmid DNA (pDNA) core, rendering it inactive.

The new nanocarrier created by Kataoka and his team is made from a layered polyplex micelle - a self-assembling structure with three distinct layers. The micelle comprises a pDNA core, followed by an intermediary layer of DPc, and finally an outer hydrophilic layer of poly(ethylene glycol), which forms a shield to protect the package during passage through the body.

The micelle structure performed well in in vivo trials on mice tumor models. The researchers used photoirradiation to ‘flick the switch' once the micelle was safely inside a tumor cell, causing a DPc-mediated chemical reaction to break the membrane seal and release the DNA into the tumor cell nucleus. Successful gene expression followed, and the team believe their new light-induced nanocarrier system could be extended to deliver therapeutic agents for all manner of diseases in future.

Publication and Affiliation

Takahiro Nomoto1, Shigeto Fukushima2, Michiaki Kumagai2, Kaori Machitani2, Arnida2, Yu Matsumoto3, Makoto Oba4, Kanjiro Miyata3, Kensuke Osada1,5, Nobuhiro Nishiyama6 & Kazunori Kataoka1,2,3. Three-layered polyplex micelle as a multifunctional nanocarrier platform for light-induced systemic gene transfer. Nature Communications 5, 3545 (2014)

1. Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
2. Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
3. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
4. Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
5. Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
6. Polymer Chemistry Division, Chemical Resources Laboratory, Tokyo Institute of Technology, R1-11, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.

####

For more information, please click here

Contacts:
corresponding author
email address:

Copyright © Kawasaki City

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Keeping electric car design on the right road: A closer look at the life-cycle impacts of lithium-ion batteries and proton exchange membrane fuel cells December 9th, 2016

Further improvement of qubit lifetime for quantum computers: New technique removes quasiparticles from superconducting quantum circuits December 9th, 2016

Chemical trickery corrals 'hyperactive' metal-oxide cluster December 8th, 2016

Scientists track chemical and structural evolution of catalytic nanoparticles in 3-D: Up-close, real-time, chemical-sensitive 3-D imaging offers clues for reducing cost/improving performance of catalysts for fuel-cell-powered vehicles and other applications December 8th, 2016

Nanomedicine

Arrowhead Pharmaceuticals to Webcast Fiscal 2016 Year End Results December 7th, 2016

Fast, efficient sperm tails inspire nanobiotechnology December 5th, 2016

Journal Nanotechnology Progress International (JONPI) Volume 6, issue 2 coming out soon! December 5th, 2016

UTSA study describes new minimally invasive device to treat cancer and other illnesses: Medicine diffusion capsule could locally treat multiple ailments and diseases over several weeks December 3rd, 2016

Discoveries

Keeping electric car design on the right road: A closer look at the life-cycle impacts of lithium-ion batteries and proton exchange membrane fuel cells December 9th, 2016

Further improvement of qubit lifetime for quantum computers: New technique removes quasiparticles from superconducting quantum circuits December 9th, 2016

Researchers peer into atom-sized tunnels in hunt for better battery: May improve lithium ion for larger devices, like cars December 8th, 2016

Scientists track chemical and structural evolution of catalytic nanoparticles in 3-D: Up-close, real-time, chemical-sensitive 3-D imaging offers clues for reducing cost/improving performance of catalysts for fuel-cell-powered vehicles and other applications December 8th, 2016

Announcements

Keeping electric car design on the right road: A closer look at the life-cycle impacts of lithium-ion batteries and proton exchange membrane fuel cells December 9th, 2016

Further improvement of qubit lifetime for quantum computers: New technique removes quasiparticles from superconducting quantum circuits December 9th, 2016

Researchers peer into atom-sized tunnels in hunt for better battery: May improve lithium ion for larger devices, like cars December 8th, 2016

Scientists track chemical and structural evolution of catalytic nanoparticles in 3-D: Up-close, real-time, chemical-sensitive 3-D imaging offers clues for reducing cost/improving performance of catalysts for fuel-cell-powered vehicles and other applications December 8th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project