Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > FEI Reports New Advances in Neuroscience in Collaboration with NIH: Using cryo-electron microscopy, researchers determine the structural mechanism of glutamate receptors – an important insight to the brain’s memory formation and learning

Abstract:
FEI is pleased to announce that researchers at the NIH-FEI Living Lab for Structural Biology have achieved breakthrough biological results, using FEI's Titan Krios™ transmission electron microscope (TEM), to elucidate the structural mechanism by which glutamate receptors participate in the transmission of signals between neurons in the brain. Their work is described in Nature, "Structural Mechanism of Glutamate Receptor Activation and Desensitization," by Meyerson, et al., (DOI: 10.1038/nature13603), www.nature.com/nature/journal/vaop/ncurrent/full/nature13603.html .

FEI Reports New Advances in Neuroscience in Collaboration with NIH: Using cryo-electron microscopy, researchers determine the structural mechanism of glutamate receptors – an important insight to the brain’s memory formation and learning

Hillsboro, OR | Posted on August 4th, 2014

"The Living Lab for Structural Biology was designed two years ago as an open innovation framework to bring together cryo-EM, nuclear magnetic resonance (NMR), x-ray diffraction (XRD), and biochemistry experts in one place to develop integrated workflows for enabling solutions to important structural biology questions," said Paul Scagnetti, vice president and general manager of FEI's Sciences Group. "This important biological research result demonstrates the success of the collaboration between FEI and the leading researchers at NIH, such as Dr. Sriram Subramaniam."

According to Sriram Subramaniam, Ph.D., senior investigator in the National Cancer Institute's Laboratory of Cell Biology and the director of the Living Lab, the prospect that the determination of structures of membrane proteins and related complexes may no longer be limited by size or by the need for crystallization suggests a critical shift in the landscape of structural biology and an exciting new horizon.

According to Mark Mayer, Ph.D., of the Laboratory of Cellular and Molecular Neurophysiology, National Institute of Child Health and Human Development (NICHD), an NIH researcher who has traditionally relied on X-ray crystallograpy to study important membrane proteins and an author on the Nature paper, iGluRs are major mediators of excitatory synaptic transmission in the brain and also play key roles in nearly all aspects of nervous system development, learning and memory. Their dysfunction is associated with major neurodegenerative and psychiatric disorders, including Alzheimer's and Parkinson's diseases, stroke, epilepsy, schizophrenia and depression. Understanding the molecular function of glutamate receptors will allow greater insight into their gating mechanisms, and allow development of therapies targeting these diseases.

A major challenge in membrane protein structural biology is the crystallization of ion channels and receptors in different functional states; with crystals in hand the most widely used approach to solve structures at atomic resolution is the collection of X-ray diffraction data. However, many membrane proteins are resistant to crystallization, and trapping them in different functional states is even more of a challenge, since the crystal lattice often selects for just one of multiple conformational states. This is especially true for glutamate receptor ion channels, which have three major conformations, a resting state, an active state, and a desensitized state, in which the receptor has bound glutamate, but no longer transmits ion channel activity. Even more challenging, glutamate receptors activate and desensitize on the millisecond time scale.

The multiple conformations of the proteins in the glutamate receptor family were solved utilizing FEI's Titan Krios TEM equipped with an XFEG and spherical aberration image corrector, Falcon™ generation II direct electron detector, and FEI EPU software, which enables 24/7 uninterrupted automated cryo-EM image collection.

Scagnetti adds, "The research relied on the automated and robust workflows for protein structure determination that were developed in the NIH-FEI Living Lab - and this was a major goal of the collaboration."

FEI announced the Living Lab Structural Biology Center at NIH in 2012 as part of a cooperative research and development agreement. Located on the NIH campus, the facility houses many FEI instruments used for biological structural determination including a Titan Krios TEM, the world's most powerful commercially-available electron microscope for structural biology. Experts from the National Cancer Institute (NCI), the Institute of Diabetes and Digestive and Kidney Diseases, (NIDDK) , and other Institutes of the NIH, such as the NICHD, and FEI, work together at the at the Living Lab to develop new methods and workflows, from sample preparation through data analysis, for cryo-electron microscopy.

For more information about FEI's microscopes for structural biology, please visit www.fei.com/life-sciences/structural-biology/. For more information about the Living Lab, please visit livinglab.nih.gov/ .

####

About FEI Company
FEI Company (Nasdaq: FEIC) designs, manufactures and supports a broad range of high-performance microscopy workflow solutions that provide images and answers at the micro-, nano- and picometer scales. Its innovation and leadership enable customers in industry and science to increase productivity and make breakthrough discoveries. Headquartered in Hillsboro, Ore., USA, FEI has over 2,600 employees and sales and service operations in more than 50 countries around the world. More information can be found at: www.fei.com.

FEI Safe Harbor Statement

This news release contains forward-looking statements that include statements regarding the performance capabilities and benefits of the NIH-FEI Living Lab, as well as the Titan Krios TEM, Falcon™ generation II direct electron detector, and FEI EPU software. Factors that could affect these forward-looking statements include but are not limited to our ability to manufacture, ship, deliver and install the tools or software as expected; failure of the product or technology to perform as expected; unexpected technology problems and challenges; changes to the technology; the inability of FEI, its suppliers or project partners to make the technological advances required for the technology to achieve anticipated results; and the inability of the customer or the NIH-FEI Living Lab to deploy the tools or develop and deploy the expected new applications or workflows. Please also refer to our Form 10-K, Forms 10-Q, Forms 8-K and other filings with the U.S. Securities and Exchange Commission for additional information on these factors and other factors that could cause actual results to differ materially from the forward-looking statements. FEI assumes no duty to update forward-looking statements.

For more information, please click here

Contacts:
Sandy Fewkes (media contact)
MindWrite Communications, Inc.
+1 408 224 4024


FEI Company
Jason Willey (investors and analysts)
Investor Relations Director
+1 503 726 2533

Copyright © FEI Company

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Download paper:

Related News Press

News and information

A nanoscale wireless communication system via plasmonic antennas: Greater control affords 'in-plane' transmission of waves at or near visible light August 27th, 2016

Forces of nature: Interview with microscopy innovators Gerd Binnig and Christoph Gerber August 26th, 2016

A promising route to the scalable production of highly crystalline graphene films August 26th, 2016

Graphene under pressure August 26th, 2016

Imaging

Forces of nature: Interview with microscopy innovators Gerd Binnig and Christoph Gerber August 26th, 2016

New approach to determining how atoms are arranged in materials August 25th, 2016

Laboratories

A new way to display the 3-D structure of molecules: Metal-organic frameworks provide a new platform for solving the structure of hard-to-study samples August 21st, 2016

Scientists uncover origin of high-temperature superconductivity in copper-oxide compound: Analysis of thousands of samples reveals that the compound becomes superconducting at an unusually high temperature because local electron pairs form a 'superfluid' that flows without resist August 19th, 2016

Let's roll: Material for polymer solar cells may lend itself to large-area processing: 'Sweet spot' for mass-producing polymer solar cells may be far larger than dictated by the conventional wisdom August 12th, 2016

NREL technique leads to improved perovskite solar cells August 11th, 2016

Govt.-Legislation/Regulation/Funding/Policy

Analog DNA circuit does math in a test tube: DNA computers could one day be programmed to diagnose and treat disease August 25th, 2016

New approach to determining how atoms are arranged in materials August 25th, 2016

Johns Hopkins scientists track metabolic pathways to find drug combination for pancreatic cancer August 25th, 2016

New electrical energy storage material shows its power: Nanomaterial combines attributes of both batteries and supercapacitors August 25th, 2016

Nanomedicine

Nanofiber scaffolds demonstrate new features in the behavior of stem and cancer cells August 25th, 2016

Johns Hopkins scientists track metabolic pathways to find drug combination for pancreatic cancer August 25th, 2016

50 years after the release of the film 'Fantastic Voyage,' science upstages fiction: Science upstages fiction with nanorobotic agents designed to travel in the human body to treat cancer August 25th, 2016

Tunneling nanotubes between neurons enable the spread of Parkinson's disease via lysosomes August 24th, 2016

Discoveries

A promising route to the scalable production of highly crystalline graphene films August 26th, 2016

Graphene under pressure August 26th, 2016

Nanofur for oil spill cleanup: Materials researchers learn from aquatic ferns: Hairy plant leaves are highly oil-absorbing / publication in bioinspiration & biomimetics / video on absorption capacity August 25th, 2016

Unraveling the crystal structure of a -70° Celsius superconductor, a world first: Significant advancement in the realization of room-temperature superconductors August 25th, 2016

Announcements

A nanoscale wireless communication system via plasmonic antennas: Greater control affords 'in-plane' transmission of waves at or near visible light August 27th, 2016

Forces of nature: Interview with microscopy innovators Gerd Binnig and Christoph Gerber August 26th, 2016

A promising route to the scalable production of highly crystalline graphene films August 26th, 2016

Graphene under pressure August 26th, 2016

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

A nanoscale wireless communication system via plasmonic antennas: Greater control affords 'in-plane' transmission of waves at or near visible light August 27th, 2016

Forces of nature: Interview with microscopy innovators Gerd Binnig and Christoph Gerber August 26th, 2016

A promising route to the scalable production of highly crystalline graphene films August 26th, 2016

New electrical energy storage material shows its power: Nanomaterial combines attributes of both batteries and supercapacitors August 25th, 2016

Tools

Nanofiber scaffolds demonstrate new features in the behavior of stem and cancer cells August 25th, 2016

50 years after the release of the film 'Fantastic Voyage,' science upstages fiction: Science upstages fiction with nanorobotic agents designed to travel in the human body to treat cancer August 25th, 2016

University of Puerto Rico and NASA back in the news – XEI reports August 23rd, 2016

Spider silk: Mother Nature's bio-superlens August 22nd, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic