- About Us
- Nano-Social Network
- Nano Consulting
- My Account
Home > Press > DNA-Linked Nanoparticles Form Switchable "Thin Films" on a Liquid Surface: Possible pathway to adjustable filters, surfaces with variable mechanical response, or even new ways to deliver genes for biomedical applications
|Click on the image to download a high-resolution version. Schematic illustration of the assembly of DNA-functionalized nanoparticles (NPs) at positively charged interfaces. (a) In the absence of salt, interactions are dominated by the electrostatic repulsion between DNA chains. (b) The 2D assemblies can be altered by programming the interactions between the NPs. By introducing monovalent salt, an attractive interaction between the NPs is switched ON, due to DNA hybridization. Change in interaction between NPs provides the path to tune the structure of the 2D assemblies at the interface.|
Scientists seeking ways to engineer the assembly of tiny particles measuring just billionths of a meter have achieved a new first-the formation of a single layer of nanoparticles on a liquid surface where the properties of the layer can be easily switched. Understanding the assembly of such nanostructured thin films could lead to the design of new kinds of filters or membranes with a variable mechanical response for a wide range of applications. In addition, because the scientists used tiny synthetic strands of DNA to hold the nanoparticles together, the study also offers insight into the mechanism of interactions of nanoparticles and DNA molecules near a lipid membrane. This understanding could inform the emerging use of nanoparticles as vehicles for delivering genes across cellular membranes.
"Our work reveals how DNA-coated nanoparticles interact and re-organize at a lipid interface, and how that process affects the properties of a "thin film" made of DNA-linked nanoparticles," said physicist Oleg Gang who led the study at the Center for Functional Nanomaterials (CFN, http://www.bnl.gov/cfn/) at the U.S. Department of Energy's Brookhaven National Laboratory. The results will be published in the June 11, 2014 print edition of the Journal of the American Chemical Society [online link: pubs.acs.org/doi/abs/10.1021/ja501749b].
Like the molecule that carries genetic information in living things, the synthetic DNA strands used as "glue" to bind nanoparticles in this study have a natural tendency to pair up when the bases that make up the rungs of the twisted-ladder shaped molecule match up in a particular way. Scientists at Brookhaven have made great use of the specificity of this attractive force to get nanoparticles coated with single synthetic DNA strands to pair up and assemble in a variety of three-dimensional architectures. The goal of the present study was to see if the same approach could be used to achieve designs of two-dimensional, one-particle-thick films.
"Many of the applications we envision for nanoparticles, such as optical coatings and photovoltaic and magnetic storage devices, require planar geometry," said Sunita Srivastava, a Stony Brook University postdoctoral researcher and the lead author on the paper. Other groups of scientists have assembled such planes of nanoparticles, essentially floating them on a liquid surface, but these single-layer arrays have all been static, she explained. "Using DNA linker molecules gives us a way to control the interactions between the nanoparticles."
As described in the paper, the scientists demonstrated their ability to achieve differently structured monolayers, from a viscous fluid-like array to a more tightly woven cross-linked elastic mesh-and switch between those different states-by varying the strength of the pairing between complementary DNA strands and adjusting other variables, including the electrostatic charge on the liquid assembly surface and the concentration of salt.
When the surface they used, a lipid, has a strong positive charge it attracts the negatively charged DNA strands that coat the nanoparticles. That electrostatic attraction and the repulsion between the negatively charged DNA molecules surrounding adjacent nanoparticles overpower the attractive force between complementary DNA bases. As a result, the particles form a rather loosely arrayed free-floating viscous monolayer. Adding salt changes the interactions and overcomes the repulsion between like-charged DNA strands, allowing the base pairs to match up and link the nanoparticles together more closely, first forming string-like arrays, and with more salt, a more solid yet elastic mesh-like layer.
"The mechanism of this phase transition is not obvious," said Gang. "It cannot be understood from the repulsion-attraction interactions alone. With the help of theory, we reveal that there are collective effects of the flexible DNA chains that drive the system in the particular states. And it is only possible when the particle sizes and the DNA chain sizes are comparable-on the order of 20-50 nanometers," he said.
As part of the study, the scientists examined the different configurations of the nanoparticles on top of the liquid layer using x-ray scattering at Brookhaven's National Synchrotron Light Source (NSLS, www.bnl.gov/ps/nsls/about-NSLS.asp). They also transferred the monolayer produced at each salt concentration to a solid surface so they could visualize it using electron microscopy at the CFN.
"Creating these particle monolayers at a liquid interface is very convenient and effective because the particles' two-dimensional structure is very 'fluid' and can be easily manipulated-unlike on a solid substrate, where the particles can easily get stuck to the surface," Gang said. "But in some applications, we may need to transfer the assembled layer to such a solid surface. By combining the synchrotron scattering and electron microscopy imaging we could confirm that the transfer can be done with minimal disruption to the monolayer."
The switchable nature of the monolayers might be particularly attractive for applications such as membranes used for purification and separations, or to control the transport of molecular or nano-scale objects through liquid interfaces. For example, said Gang, when particles are linked but move freely at the interface, they may allow an object-a molecule-to pass through the interface. "However, when we induce linkages between particles to form a mesh-like network, any object larger than the mesh-size of the network cannot penetrate through this very thin film. "
"In principle, we can even think about such on-demand regulated networks to adjust the mesh size dynamically. Because, of the nanoscale size-regime, we might envision using such membranes for filtering proteins or other nanoparticles," he said.
Understanding how synthetic DNA-coated nanoparticles interact with a lipid surface may also offer insight into how such particles coated with actual genes might interact with cell membranes-which are largely composed of lipids-and with one another in a lipid environment.
"Other groups have considered using DNA-coated nanoparticles to detect genes within cells, or even for delivering genes to cells for gene therapy and such approaches," said Gang. "Our study is the first of its kind to look at the structural aspects of DNA-particle/lipid interface directly using x-ray scattering. I believe this approach has significant value as a platform for more detailed investigations of realistic systems important for these new biomedical applications of DNA-nanoparticle pairings," Gang said.
This research was sponsored by the DOE Office of Science (BES).
DOE's Office of Science is the single largest supporter of basic research in the physical sciences in the United States, and is working to address some of the most pressing challenges of our time. For more information, please visit science.energy.gov.
The Center for Functional Nanomaterials at Brookhaven National Laboratory is one of the five DOE Nanoscale Science Research Centers (NSRCs), premier national user facilities for interdisciplinary research at the nanoscale. Together the NSRCs comprise a suite of complementary facilities that provide researchers with state-of-the-art capabilities to fabricate, process, characterize and model nanoscale materials, and constitute the largest infrastructure investment of the National Nanotechnology Initiative. The NSRCs are located at DOE's Argonne, Brookhaven, Lawrence Berkeley, Oak Ridge and Sandia and Los Alamos national laboratories. For more information about the DOE NSRCs, please visit nano.energy.gov.
About Brookhaven National Laboratory
One of ten national laboratories overseen and primarily funded by the Office of Science of the U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical, biomedical, and environmental sciences, as well as in energy technologies and national security. Brookhaven Lab also builds and operates major scientific facilities available to university, industry and government researchers. Brookhaven is operated and managed for DOE's Office of Science by Brookhaven Science Associates, a limited-liability company founded by the Research Foundation for the State University of New York on behalf of Stony Brook University, the largest academic user of Laboratory facilities, and Battelle, a nonprofit applied science and technology organization.
For more information, please click here
Karen McNulty Walsh
Copyright © Brookhaven National LaboratoryIf you have a comment, please Contact us.
Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.
|Related News Press|
News and information
Leti Presents Advances in Propagation Modeling and Antenna Design for mmWave Spectrum: Paper Is One of 15 that Leti Presented at European Conference on Antennas and Propagation March 19-24 March 23rd, 2017
Researchers develop groundbreaking process for creating ultra-selective separation membranes: Discovery could greatly improve energy-efficiency of separation and purification processes in the chemical and petrochemical industries March 15th, 2017
Imaging the inner workings of a sodium-metal sulfide battery for first time: Understanding how the structural and chemical makeup of the material changes during the charge/discharge process could help scientists advance battery design for future energy storage needs March 9th, 2017
Dual-function nanorod LEDs could make multifunctional displays February 11th, 2017
NREL research pinpoints promise of polycrystalline perovskites February 8th, 2017
ANU invention to inspire new night-vision specs December 7th, 2016
Nanocages for gold particles: what is happening inside? March 16th, 2017
Rare-earths become water-repellent only as they age March 22nd, 2017
Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers
AIM Photonics Welcomes Coventor as Newest Member: US-Backed Initiative Taps Process Modeling Specialist to Enable Manufacturing of High-Yield, High-Performance Integrated Photonic Designs March 16th, 2017
Leti Coordinating Project to Adapt Obstacle-Detection Technology Used in Autonomous Cars for Portable and Wearable Systems: INSPEX to Combine Knowhow of Nine European Organizations to Create Portable and Wearable Spatial-Exploration Systems February 2nd, 2017