Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > New live-cell printing technology works like ancient Chinese woodblocking

This image shows cells printed in a grid pattern by block cell printing technology (left) and woodblocks used in ancient Chinese printing (right).

Credit: Lidong Qin lab and Digital Museum of Science and Art (Beijing, China)
This image shows cells printed in a grid pattern by block cell printing technology (left) and woodblocks used in ancient Chinese printing (right).

Credit: Lidong Qin lab and Digital Museum of Science and Art (Beijing, China)

Abstract:
With a nod to 3rd century Chinese woodblock printing and children's rubber stamp toys, researchers in Houston have developed a way to print living cells onto any surface, in virtually any shape. Unlike recent, similar work using inkjet printing approaches, almost all cells survive the process, scientists report in this week's Proceedings of the National Academy of Sciences.

New live-cell printing technology works like ancient Chinese woodblocking

Houston, TX | Posted on February 10th, 2014

The researchers, led by Houston Methodist Research Institute nanomedicine faculty member Lidong Qin, Ph.D., say their approach produces 2-D cell arrays in as little as half an hour, prints the cells as close together as 5 micrometers (most animal cells are 10 to 30 micrometers wide), and allows the use of many different cell types. They've named the technology Block-Cell-Printing, or BloC-Printing.

"We feel the current technologies are inadequate," Qin said. "Inkjet-based cell printing leaves many of the cells damaged or dead. We wanted to see if we could invent a tool that helps researchers obtain arrays of cells that are alive and still have full activity."

Recent work to print cells in two and three dimensions using electricity-gated inkjet technology have been largely successful, but sometimes only half of the printed cells survive the printing process -- a source of frustration for many laboratory scientists.

"Cell printing is used in so many different ways now -- for drug development and in studies of tissue regeneration, cell function, and cell-cell communication," Qin said. "Such things can only be done when cells are alive and active. A survival rate of 50 to 80 percent is typical as cells exit the inkjet nozzles. By comparison, we are seeing close to 100 percent of cells in BloC-Printing survive the printing process."

BloC-Printing manipulates microfluidic physics to guide living cells into hook-like traps in the silicone mold. Cells flow down a column in the mold, past trapped cells to the next available slot, eventually creating a line of cells (in a grid of such lines). The position and spacing of the traps and the shape of the channel navigated by the cells is fully configurable during the mold's creation. When the mold is lifted away, the living cells remain behind, adhering to the growth medium or other substrate, in prescribed formation.

Qin's group tested BloC-Printing for its utility in studying cancerous cells and primary neurons. By arranging metastatic cancer cells in a grid and examining their growth in comparison with a non-metastatic control, the researchers found they could easily characterize the metastatic potential of cancer cells.

"We looked at cancer cells for their protrusion generation capability, which correlates to their malignancy level," Qin said. "Longer protrusion means more aggressive cancer cells. The measurement may help to diagnose a cancer's stage."

The researchers also printed a grid of brain cells and gave the cells time to form synaptic and autaptic junctions.

"The cell junctions we created may be useful for future neuron signal transduction and axon regeneration studies," Qin said. "Such work could be helpful in understanding Alzheimer's disease and other neurodegenerative diseases."

While it is too early to predict the market cost of BloC-Printing, Qin said the materials of a single BloC mold cost about $1 (US). After the mold has been fabricated and delivered, a researcher only needs a syringe, a carefully prepared suspension of living cells, a Petri dish, and a steady hand, Qin said. Inkjet cell printers can cost between $10,000 and $200,000.

"BloC-Printing can be combined with molecular printing for many types of drug screening, RNA interference, and molecule-cell interaction studies," he said. "We believe the technology has big potential."

While the fidelity of BloC-Printing is high, Qin said inkjet printing remains faster, and BloC-Printing cannot yet print multi-layer structures as inkjetting can.

###

Qin and postdoctoral fellow Kai Zhang, Ph.D., are BloC-Printing's co-inventors.

Qin and Zhang's PNAS coauthors are Chao-Kai Chou, Ph.D., and Mien-Chie Hung, Ph.D., (the University of Texas M.D. Anderson Cancer Center), and Xiaofeng Xia, Ph.D. (Houston Methodist Research Institute). The researchers acknowledge support from the National Institutes of Health, the Cancer Prevention and Research Institute of Texas, the U.S. Dept. of Defense, the Emily Hermann Research Fund, the Golfers Against Cancer, and the Alliance for Nanohealth.

In addition to his position in the Houston Methodist Research Institute's Department of Nanomedicine, Qin is also a Weill Cornell Medical College assistant professor of cell and developmental biology.

####

For more information, please click here

Contacts:
David Bricker

832-667-5811

Copyright © Houston Methodist

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

In IEDM 2016 Keynote, Leti CEO Says ‘Hyperconnectivity’, Human-focused Research and the IOT Promise Profound, Positive Changes December 7th, 2016

Leti IEDM 2016 Paper Clarifies Correlation between Endurance, Window Margin and Retention in RRAM for First Time: Paper Presented at IEDM 2016 Offers Ways to Reconcile High-cycling Requirements and Instability at High Temperatures in Resistive RAM December 6th, 2016

Tokyo Institute of Technology research: 3D solutions to energy savings in silicon power transistors December 6th, 2016

Physicists decipher electronic properties of materials in work that may change transistors December 6th, 2016

Govt.-Legislation/Regulation/Funding/Policy

Physicists decipher electronic properties of materials in work that may change transistors December 6th, 2016

Construction of practical quantum computers radically simplified: Scientists invent ground-breaking new method that puts quantum computers within reach December 5th, 2016

Shape matters when light meets atom: Mapping the interaction of a single atom with a single photon may inform design of quantum devices December 4th, 2016

Research Study: MetaSOLTM Shatters Solar Panel Efficiency Forecasts with Innovative New Coating: Coating Provides 1.2 Percent Absolute Enhancement to Triple Junction Solar Cells December 2nd, 2016

Nanomedicine

Fast, efficient sperm tails inspire nanobiotechnology December 5th, 2016

Journal Nanotechnology Progress International (JONPI) Volume 6, issue 2 coming out soon! December 5th, 2016

UTSA study describes new minimally invasive device to treat cancer and other illnesses: Medicine diffusion capsule could locally treat multiple ailments and diseases over several weeks December 3rd, 2016

Nanobiotix Provides Update on Global Development of Lead Product NBTXR3: Seven clinical trials across the world: More than 2/3 of STS patients recruited in the “act.in.sarc” Phase II/III trial: Phase I/II prostate cancer trial now recruiting in the U.S. November 28th, 2016

Discoveries

Leti IEDM 2016 Paper Clarifies Correlation between Endurance, Window Margin and Retention in RRAM for First Time: Paper Presented at IEDM 2016 Offers Ways to Reconcile High-cycling Requirements and Instability at High Temperatures in Resistive RAM December 6th, 2016

Tokyo Institute of Technology research: 3D solutions to energy savings in silicon power transistors December 6th, 2016

Physicists decipher electronic properties of materials in work that may change transistors December 6th, 2016

Fast, efficient sperm tails inspire nanobiotechnology December 5th, 2016

Announcements

In IEDM 2016 Keynote, Leti CEO Says ‘Hyperconnectivity’, Human-focused Research and the IOT Promise Profound, Positive Changes December 7th, 2016

Leti IEDM 2016 Paper Clarifies Correlation between Endurance, Window Margin and Retention in RRAM for First Time: Paper Presented at IEDM 2016 Offers Ways to Reconcile High-cycling Requirements and Instability at High Temperatures in Resistive RAM December 6th, 2016

Tokyo Institute of Technology research: 3D solutions to energy savings in silicon power transistors December 6th, 2016

Physicists decipher electronic properties of materials in work that may change transistors December 6th, 2016

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Tokyo Institute of Technology research: 3D solutions to energy savings in silicon power transistors December 6th, 2016

Physicists decipher electronic properties of materials in work that may change transistors December 6th, 2016

Fast, efficient sperm tails inspire nanobiotechnology December 5th, 2016

Journal Nanotechnology Progress International (JONPI) Volume 6, issue 2 coming out soon! December 5th, 2016

Grants/Awards/Scholarships/Gifts/Contests/Honors/Records

Physicists decipher electronic properties of materials in work that may change transistors December 6th, 2016

Shape matters when light meets atom: Mapping the interaction of a single atom with a single photon may inform design of quantum devices December 4th, 2016

Quantum obstacle course changes material from superconductor to insulator December 1st, 2016

'Back to the Future' inspires solar nanotech-powered clothing November 15th, 2016

Nanobiotechnology

Fast, efficient sperm tails inspire nanobiotechnology December 5th, 2016

Deep insights from surface reactions: Researchers use Stampede supercomputer to study new chemical sensing methods, desalination and bacterial energy production December 2nd, 2016

Nanobiotix Provides Update on Global Development of Lead Product NBTXR3: Seven clinical trials across the world: More than 2/3 of STS patients recruited in the “act.in.sarc” Phase II/III trial: Phase I/II prostate cancer trial now recruiting in the U.S. November 28th, 2016

From champagne bubbles, dance parties and disease to new nanomaterials: Understanding nucleation of protein filaments might help with Alzheimer's Disease and type 2 Diabetes November 24th, 2016

Printing/Lithography/Inkjet/Inks/Bio-printing

Bumpy surfaces, graphene beat the heat in devices: Rice University theory shows way to enhance heat sinks in future microelectronics November 29th, 2016

Engineers develop new magnetic ink to print self-healing devices that heal in record time November 7th, 2016

Iran to hold intl. school on application of nanomaterials in medicine September 20th, 2016

Tailored probes for atomic force microscopes: 3-D laser lithography enhances microscope for studying nanostructures in biology and engineering/ publication in Applied Physics Letters August 11th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project