Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Phasefocus reports on users at the University of York - New Publication in Nature Scientific Reports

Dr Rakesh Suman uses the Phasefocus VL20 system at the University of York
Dr Rakesh Suman uses the Phasefocus VL20 system at the University of York

Abstract:
Phase Focus Ltd (Phasefocus), the company that is revolutionizing microscopy and imaging with the Phasefocus Virtual Lens® technology, reports on an exciting publication in Nature Scientific Reports by their users from the University of York's Bioscience Technology Facility.

Phasefocus reports on users at the University of York - New Publication in Nature Scientific Reports

Sheffield, UK | Posted on January 28th, 2014

Phase Focus Ltd (Phasefocus), the company that is revolutionizing microscopy and imaging with the Phasefocus Virtual Lens® technology, is pleased to report on a new paper published in Nature Scientific Reports describing their patented technology based on ptychography: "Ptychography - a label free, high-contrast imaging technique for live cells using quantitative phase information" is an open access paper written by a team of scientists from the Imaging and Cytometry Laboratory, part of the Bioscience Technology Facility in the Department of Biology at the University of York. The lead authors are Joanne Marrison and Peter O'Toole, the latter being head of the Laboratory.1

Quoting from their summary, the authors describe the motivation for this work: "Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is particularly suitable for reporting cellular changes such as mitosis, apoptosis and cell differentiation. The high contrast, artefact-free, focus-free information rich images allow dividing cells to be distinguished from non-dividing cells by a greater than two-fold increase in cell contrast, and we demonstrate this technique is suitable for downstream automated cell segmentation and analysis."

The authors conclude that this has tremendous potential. "The system generates high quality images using inexpensive, low magnification objective lenses without the need for the addition of cytotoxic labels, a technical advance which has far reaching implications for cancer research and drug discovery. Not only are the images high in contrast, but they are also artefact free making them ideal for downstream segmentation and image analysis. The ability to focus post-acquisition allows all cells, even in an uneven field, to be brought into focus and not omitted from subsequent analysis. Time is also saved pre-acquisition as cells can be imaged directly in tissue culture plastic ware. We have shown here that this technique is suitable for cell cycle analysis, identifying cells entering and exiting mitosis and dissecting the phases of mitosis. Ptychography would be ideal for in-incubator assays, reporting over prolonged periods of time on cell proliferation and cell cycle stages in situ. These are only initial studies but clearly demonstrate the potential of label-free ptychographic imaging. With the technology rapidly developing, faster acquisition times, smart auto-focus, increased contrast and ease of use, and the ability to add the facility to other systems e.g. confocal microscopes, make the future opportunities diverse and very attractive."

Dr O'Toole describes ptychography as "one of the most important breakthroughs in imaging. It addresses many of the fundamental problems inherent in current microscopy techniques. Put simply, what you get is far more than what you see. Artefacts of sample preparation which may arise from labelling or use of markers are negated. I have been most impressed by Phasefocus and their ability to quickly respond to feedback and then to develop products to meet the needs of the user. I wonder where the technology will go? I feel the journey is just beginning and microscopy is nowhere near the end of the road with regards to development."

For more information on ptychography and its applications, please visit the Phasefocus web site: www.phasefocus.com.

####

About Phase Focus Ltd
Phasefocus is a venture capital-backed spin-out company from the University of Sheffield founded in 2006. Its principal equity investors are Ombu Group and Fusion IP plc. In February 2013, the Company announced that it had entered into a Licencing Agreement with Gatan Inc., the world's leading manufacturer of instrumentation and software used to enhance and extend the operation and performance of electron microscopes. A prototype "add-on" product for existing electron microscopes has already demonstrated an improvement in resolution of a factor of five over that available using the host instrument's conventional electron optics. Its products also include an optical microscope used for label-free time-lapse imaging of biological cells, and an Ophthalmic Lens Profiler that can measure soft contact lenses with unprecedented levels of resolution and accuracy.

For more information, please click here

Contacts:
Phase Focus Limited
Electric Works
Sheffield Digital Campus
Sheffield, S1 2BJ, UK
T +44(0)114 286 6377
M +44(0)7985 042392
www.phasefocus.com


Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997
www.talking-science.com

Copyright © Phase Focus Ltd

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Reference - the full article is available online at:

Related News Press

News and information

Conductive Inks: booming to $2.8 billion by 2024 April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

INSCX™ exchange to present Exchange trade reporting mechanism for engineered nanomaterials (NMs) to UK regulation agencies, insurers and upstream/downstream users April 17th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Imaging

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

Announcements

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

INSCX™ exchange to present Exchange trade reporting mechanism for engineered nanomaterials (NMs) to UK regulation agencies, insurers and upstream/downstream users April 17th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals

Conductive Inks: booming to $2.8 billion by 2024 April 17th, 2014

Novel stapled peptide nanoparticle combination prevents RSV infection, study finds April 17th, 2014

Thinnest feasible membrane produced April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

Tools

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

Scientists Capture Ultrafast Snapshots of Light-Driven Superconductivity: X-rays reveal how rapidly vanishing 'charge stripes' may be behind laser-induced high-temperature superconductivity April 16th, 2014

Aerotech X-Y ball-screw stage for economical high performance Planar positioning April 16th, 2014

Malvern reports on the publication of the 1000th peer-reviewed paper to cite NanoSight’s Nanoparticle Tracking Analysis, NTA April 16th, 2014

Patents/IP/Tech Transfer/Licensing

Harris & Harris Group Notes Mersana's Collaboration Agreement With Subsidiary of Takeda Pharmaceutical Co. April 8th, 2014

Nanoparticles cause cancer cells to self-destruct April 3rd, 2014

A*STAR's Simtech collaboration agreements to accelerate the growth and development of the microfluidics industry April 1st, 2014

Dolomite releases novel droplet-on-demand sequencing and droplet generation microfluidic system April 1st, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE