Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > New Malvern application note illustrates how Viscotek SEC-MALS 20 measures protein aggregation

Using the Viscotek SEC-MALS 20 from Malvern to measure protein aggregation in pepsin
Using the Viscotek SEC-MALS 20 from Malvern to measure protein aggregation in pepsin

Abstract:
A new application note exploring the measurement of protein aggregation using the recently launched Viscotek SEC-MALS 20 light scattering detector is now available on the Malvern Instruments website. ‘Measuring protein aggregation with the Viscotek SEC-MALS 20' describes the use of the new system in size exclusion chromatography (SEC) analysis of a variety of protein samples, including bovine serum albumin, thyroglobulin and pepsin. These data demonstrate the detector's ability to simultaneously and accurately measure molecular weight for both larger (>10 - 15 nm) and smaller sized components within a mixture, an important feature for aggregation studies. The new application note can be downloaded from the Malvern website at www.malvern.com/secmalslaunch.

New Malvern application note illustrates how Viscotek SEC-MALS 20 measures protein aggregation

Malvern, UK | Posted on September 18th, 2013

Protein aggregation is recognized as a major issue in the biopharmaceutical industry. Proteins have a tendency to aggregate over time and under a variety of conditions. In clinical use as therapeutics, this presents a risk of them becoming not only ineffective but potentially immunogenic. In the highly dynamic environment of biopharmaceutical development, with its evolving regulatory concerns, many different analytical techniques are used to examine protein aggregation but SEC-MALS is already an established approach.

Data from the Viscotek SEC-MALS 20 is used in calculations of both molecular weight and radius of gyration. Light scattering detectors are valued for their ability to directly determine absolute molecular weight but this is easier for smaller molecules, which scatter light isotropically, than for larger, anisotropic scatterers. By incorporating detectors at 20 angles, including one at 90o, the Viscotek SEC-MALS 20 produces accurate data for molecules in a wide range of sizes, maximizing its value for applications where differently sized components are present.

The Viscotek SEC-MALS 20 system offers more detectors and higher accuracy than any other commercially available MALS detector. An innovative vertical flow cell with radial optics allows the use of any mobile phase without the need to alter optical properties of the detectors. Operating via Malvern's established OmniSEC software, the Viscotek SEC-MALS 20 is available as a standalone unit, compatible with any commercially available GPC/SEC set up, or integrated within multi detection Viscotek systems. Its recent addition to Malvern's Viscotek range means that the company now offers the most comprehensive choice of light scattering detectors for GPC/SEC analysis, including options for low angle, right angle and multi angle scattering.

Download the application note ‘Measuring protein aggregation with the Viscotek SEC-MALS 20' at www.malvern.com/secmalslaunch.

Malvern, Malvern Instruments, Viscotek are registered trademarks of Malvern Instruments Ltd

####

About Malvern Instruments
Malvern provides the materials and biophysical characterization technology and expertise that enables scientists and engineers to understand and control the properties of dispersed systems. These systems range from proteins and polymers in solution, particle and nanoparticle suspensions and emulsions, through to sprays and aerosols, industrial bulk powders and high concentration slurries. Used at all stages of research, development and manufacturing, Malvern’s materials characterization instruments provide critical information that helps accelerate research and product development, enhance and maintain product quality and optimize process efficiency.

Our products reflect Malvern’s drive to exploit the latest technological innovations and our commitment to maximizing the potential of established techniques. They are used by both industry and academia, in sectors ranging from pharmaceuticals and biopharmaceuticals to bulk chemicals, cement, plastics and polymers, energy and the environment.

Malvern systems are used to measure particle size, particle shape, zeta potential, protein charge, molecular weight, mass, size and conformation, rheological properties and for chemical identification, advancing the understanding of dispersed systems across many different industries and applications.

Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Japan and Korea, a joint venture in India, a global distributor network and applications laboratories around the world. www.malvern.com

Facebook: MalvernInstruments
Blog: www.materials-talks.com
Youtube: malverninstruments
Linked-In: malvern-instruments
Twitter: malvern_news
Google +: https://plus.google.com/105749461389316404960/posts#105749461389316404960/posts

For more information, please click here

Contacts:
For press information, please contact:

Trish Appleton
Kapler Communications
Phoenix House, Phoenix Park
Eaton Socon, Cambridgeshire, PE19 8EP, UK
Tel: +44 (0)1480 471059
Fax: +44 (0)1480 471069


USA contact:

Marisa Fraser
Malvern Instruments Inc.
117 Flanders Road
Westborough, MA 01581-1042 USA
Tel: +1 508 768 6400
Fax: +1 508 768 6403


Please send sales enquiries to:

Alison Vines
Malvern Instruments Ltd
Enigma Business Park, Grovewood Road
Malvern, Worcestershire WR14 1XZ UK
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789

Copyright © Malvern Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Quantum twisted Loong confirms the physical reality of wavefunctions September 23rd, 2017

Application of air-sensitive semiconductors in nanoelectronics: 2-D semiconductor gallium selenide in encapsulated nanoelectronic devices September 22nd, 2017

Researchers set time limit for ultrafast perovskite solar cells September 22nd, 2017

DNA triggers shape-shifting in hydrogels, opening a new way to make 'soft robots' September 21st, 2017

Software

Leti Launches Emulator Service to Boost ROI and Speed Time to Market for European Chipmakers: Anchored by Mentor Veloce Emulator Machine, Leti’s Offer Includes Support for Design, Debug and Analysis of Results August 31st, 2017

Technology Companies Join Forces for TEM Imaging and Analysis August 3rd, 2017

Nanometrics Introduces SpectraProbe Analysis Software: Advanced software and algorithms enhancing Nanometrics metrology fleet capabilities fab-wide July 13th, 2017

Nanometrics Releases NanoDiffract 4: Latest software extends process control capabilities for advanced 3D devices July 11th, 2017

Discoveries

Quantum twisted Loong confirms the physical reality of wavefunctions September 23rd, 2017

Application of air-sensitive semiconductors in nanoelectronics: 2-D semiconductor gallium selenide in encapsulated nanoelectronic devices September 22nd, 2017

Researchers set time limit for ultrafast perovskite solar cells September 22nd, 2017

DNA triggers shape-shifting in hydrogels, opening a new way to make 'soft robots' September 21st, 2017

Announcements

Quantum twisted Loong confirms the physical reality of wavefunctions September 23rd, 2017

Application of air-sensitive semiconductors in nanoelectronics: 2-D semiconductor gallium selenide in encapsulated nanoelectronic devices September 22nd, 2017

Researchers set time limit for ultrafast perovskite solar cells September 22nd, 2017

DNA triggers shape-shifting in hydrogels, opening a new way to make 'soft robots' September 21st, 2017

Tools

Graphene based terahertz absorbers: Printable graphene inks enable ultrafast lasers in the terahertz range September 13th, 2017

Chemical hot spots: Scanning tunneling microscopy measurements identify active sites on catalyst surfaces September 7th, 2017

Phenom-World selects Deben to supply a tensile stage as an accessory to their range of desktop SEMs August 29th, 2017

New results reveal high tunability of 2-D material: Berkeley Lab-led team also provides most precise band gap measurement yet for hotly studied monolayer moly sulfide August 26th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project