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Home > Press > University of Cordoba research group publishes Nanoparticle Tracking Analysis data on metallic nanoparticles as part of a program to develop sustainable nanomaterials

A NanoSight LM-10 system as used by the University of Cordoba.
A NanoSight LM-10 system as used by the University of Cordoba.

Abstract:
NanoSight reports on how the University of Cordoba has applied Nanoparticle Tracking Analysis, NTA, to improve the understanding of the behaviour of silver colloids used in the development of sustainable nanomaterials.

University of Cordoba research group publishes Nanoparticle Tracking Analysis data on metallic nanoparticles as part of a program to develop sustainable nanomaterials

Salisbury, UK | Posted on September 17th, 2013

Dr Rafael Luque is a research member in the Department of Organic Chemistry on the Campus de Rabanales of the University of Cordoba in Spain. The main research goals of his team include the preparation of nanoparticle systems for potential utilization as catalysts and in other applications (e.g. antimicrobial and photocatalytic activities). They are focused on the design of various nanomaterials for a broad range of applications.

Dr Luque used NTA to demonstrate the successful preparation of silver colloid nanoparticles using biopolymer derived capping agents. It proved most successful resulting in a new paper published by RSC Advances entitled "Evaluation of biomass-derived stabilising agents for colloidal silver nanoparticles via nanoparticle tracking analysis (NTA)." Here, Dr Luque was able to confirm the presence and distribution of the nanoparticles by NTA using transmission electron microscopy, TEM.

Describing his work, Dr Luque said "We have extensively employed TEM to ascertain structures and NP sizes of all types of materials. This is one of the most widely employed techniques for NP characterization in terms of size and shape. With our discovery of NTA, we now see a methodology that is robust, simple and quick to use. This simplicity and effectiveness to generate results in our highly focused projects is a very welcome advantage."

Discussing the research paper* in more depth, Dr Luque described how NTA was used to differentiate between the capping efficiencies of various biomass-derived stabilising agents (e.g. starch, alginic acid and a waste-derived biorefinery sugar syrup) of aqueous colloidal silver suspensions. The results indicated that the use of a complex, biorefinery hemicellulosic-derived syrup containing a mixture of C5 and C6 sugars, as well as oligomers, provided comparable capping and stabilization properties to those of the most efficient pure polysaccharides including alginic acid. He concluded, "These findings illustrate the potential of waste-derived feedstocks for the stabilization of nanoparticles in solution."

To find out about the company and to learn more about particle characterization using NanoSight's unique Nanoparticle Tracking Analysis solutions, visit www.nanosight.com/ and register to receive the next issue of NanoTrail, the company's electronic newsletter.

*Reference: R Luque et al, "Evaluation of biomass-derived stabilising agents for colloidal silver nanoparticles via nanoparticle tracking analysis (NTA)," RSC Advances 2013, 3, 7119-7123.

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About NanoSight
NanoSight delivers the world's most versatile and proven multi-parameter nanoparticle analysis in a single instrument.

NanoSight's "Nanoparticle Tracking Analysis" (NTA) detects and visualizes populations of nanoparticles in liquids down to 10 nm, dependent on material, and measures the size of each particle from direct observations of diffusion. Additionally, NanoSight measures concentration and a fluorescence mode differentiates suitably-labelled particles within complex background suspensions. Zeta potential measurements are similarly particle-specific. It is this particle-by-particle methodology that takes NTA beyond traditional light scattering and other ensemble techniques in providing high-resolution particle size distributions and validates data with information-rich video files of the particles moving under Brownian motion.

This simultaneous multiparameter characterization matches the demands of complex biological systems, hence its wide application in development of drug delivery systems, of viral vaccines, and in nanotoxicology. This real-time data gives insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner. NanoSight has a growing role in biodiagnostics, being proven in detection and speciation of nanovesicles (exosomes) and microvesicles.

NanoSight has installed more than 600 systems worldwide with users including BASF, GlaxoSmithKline, Merck, Novartis, Pfizer, Proctor and Gamble, Roche and Unilever together with the most eminent universities and research institutes. NanoSight's technology is validated by 900+ third party papers citing NanoSight results. NanoSight's leadership position in nanoparticle characterization is consolidated further with publication of an ASTM International standard, ASTM E2834, which describes the NTA methodology for detection and analysis of nanoparticles.

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