Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Nature: Watching Molecule Movements in Live Cells - KIT Scientists Combine STED and RICS Microscopy Methods/Publication in Nature Communications

The STED-RICS microscope scans the fluorescent cell membrane with a light spot and, thus, an image is recorded.Figure: P. N. Hedde/KIT
The STED-RICS microscope scans the fluorescent cell membrane with a light spot and, thus, an image is recorded.

Figure: P. N. Hedde/KIT

Abstract:
The newly developed STED-RICS microscopy method records rapid movements of molecules in live samples. By combining raster image correlation spectroscopy (RICS) with STED fluorescence microscopy, researchers of Karlsruhe Institute of Technology (KIT) opened up new applications in medical research, e.g. analyzing the dynamics of cell membranes at high protein concentrations. This method is now presented in Nature Communications (doi: 10.1038/ncomms3093).

Nature: Watching Molecule Movements in Live Cells - KIT Scientists Combine STED and RICS Microscopy Methods/Publication in Nature Communications

Karlsruhe, Germany | Posted on July 24th, 2013

How do individual biomolecules move in live cells, tissues, or organisms? How do the biomolecules interact? These questions have to be answered to better understand the processes of life on the molecular level. STED fluorescence microscopy allows to pursue the movements and interactions of biomolecules in live samples with high spatial and temporal resolution. For this purpose, the structures to be studied are marked selectively using fluorescent dyes. Then, changes with time can be videotaped. However, the image sequence is rather slow, such that rapid molecule movements cannot be recorded directly. A group of KIT researchers around Professor Gerd Ulrich Nienhaus from the Institute of Applied Physics (APH) and the Center for Functional Nanostructures (CFN) now presents a new method to measure such rapid molecule movements in live samples in the Nature Communications journal.

The new method combines two types of microscopy. Using a confocal scanning microscope, fluorescence images are recorded point by point at fixed time intervals. Hence, the images contain an implicit time structure. This information can be used with the help of raster image correlation spectroscopy (RICS) to determine the dynamics of biomolecules, such as proteins, in live cells or tissue samples. However, protein concentrations often are too high to apply RICS together with conventional microscopy. For this reason, the KIT researchers combined the RICS method with STED microscopy (stimulated emission depletion microscopy). When using STED, the light spot scanning the fluorescence image can be reduced considerably. This method has already been used successfully to reach a maximum resolution in the imaging of cells. A STED microscope is a fluorescence microscope, whose resolution is not limited by the Abbe limit.

By combining raster image correlation spectroscopy with STED microscopy, KIT researchers have now succeeded in quantifying molecule dynamics in biological structures based on the raster images recorded. "This means that the STED-RICS method can be used to derive from any fluorescence image a highly resolved map of the number and movability of the marked molecules in the area scanned by the spot," Gerd Ulrich Nienhaus explains.

Professor Nienhaus' working group consists of physicists, chemists, and biologists. Interdisciplinary cooperation is indispensable to cover all aspects when developing new microscopic instruments and methods for biophysical fundamental research. When applications are addressed, other KIT researchers join the team and contribute their knowledge of molecular processes. In the case of the STED-RICS method, the team worked together with scientists from the Institute of Toxicology and Genetics (ITG) and the Cell and Developmental Biology Division of the Zoological Institute.

The STED-RICS method opens up new measurement applications in life sciences. A major application is research into the dynamics of cell membranes. Numerous receptor proteins are embedded in the membranes. By interaction with externally docking ligand molecules, external signals are transmitted into the cell. With the help of STED-RICS, the researchers can now determine precisely and quantitatively the movements of both lipids and receptors. Understanding of these processes is of crucial importance to medical and pharmaceutical research. Many pharmaceutical substances are based on influencing these interactions. "About every second medical substance influences signal transduction of G-protein coupled receptors, an important sub-class," Professor Nienhaus explains.

Per Niklas Hedde, René M. Dörlich, Rosmarie Blomley, Dietmar Gradl, Emmanuel Oppong, Andrew C.B. Cato & G. Ulrich Nienhaus: Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells. Nature Communications 4. 2013. Article number: 2093. doi:10.1038/ncomms3093.

####

About Helmholtz Association of German Research Centres
The Helmholtz Association is dedicated to pursuing the long-term research goals of state and society, and to maintaining and improving the livelihoods of the population. In order to do this, the Helmholtz Association carries out top-level research to identify and explore the major challenges facing society, science and the economy. Its work is divided into six strategic research fields: Energy; Earth and Environment; Health; Key Technologies; Structure of Matter; and Aeronautics, Space and Transport. The Helmholtz Association brings together 18 scientific-technical and biological-medical research centres. With some 32,698 employees and an annual budget of approximately €3.4 billion, the Helmholtz Association is Germany’s largest scientific organisation. Its work follows in the tradition of the great natural scientist Hermann von Helmholtz (1821-1894).

About Karlsruhe Institute of Technology

Karlsruhe Institute of Technology (KIT) is a public corporation according to the legislation of the state of Baden-Württemberg. It fulfills the mission of a university and the mission of a national research center of the Helmholtz Association. Research activities focus on energy, the natural and built environment as well as on society and technology and cover the whole range extending from fundamental aspects to application. With about 9000 employees, including nearly 6000 staff members in the science and education sector, and 24000 students, KIT is one of the biggest research and education institutions in Europe. Work of KIT is based on the knowledge triangle of research, teaching, and innovation.

For more information, please click here

Contacts:
Monika Landgraf
Chief Press Officer

49-721-608-47414
Fax: +49 721 608-43658

Kosta Schinarakis
PKM – Themenscout
Tel.: +49 721 608 41956
Fax: +49 721 608 43658

Copyright © Helmholtz Association of German Research Centres

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

A big nano boost for solar cells: Kyoto University and Osaka Gas effort doubles current efficiencies January 21st, 2017

A toolkit for transformable materials: How to design materials with reprogrammable shape and function January 20th, 2017

Explaining how 2-D materials break at the atomic level January 20th, 2017

New research helps to meet the challenges of nanotechnology: Research helps to make the most of nanoscale catalytic effects for nanotechnology January 20th, 2017

Imaging

Chemists Cook up New Nanomaterial and Imaging Method: Nanomaterials can store all kinds of things, including energy, drugs and other cargo January 19th, 2017

Distinguishing truth under the surface: electrostatic or mechanic December 31st, 2016

Nanoscale 'conversations' create complex, multi-layered structures: New technique leverages controlled interactions across surfaces to create self-assembled materials with unprecedented complexity December 22nd, 2016

Safe and inexpensive hydrogen production as a future energy source: Osaka University researchers develop efficient 'green' hydrogen production system that operates at room temperature in air December 21st, 2016

Nanomedicine

New research helps to meet the challenges of nanotechnology: Research helps to make the most of nanoscale catalytic effects for nanotechnology January 20th, 2017

Chemists Cook up New Nanomaterial and Imaging Method: Nanomaterials can store all kinds of things, including energy, drugs and other cargo January 19th, 2017

'5-D protein fingerprinting' could give insights into Alzheimer's, Parkinson's January 19th, 2017

New active filaments mimic biology to transport nano-cargo: A new design for a fully biocompatible motility engine transports colloidal particles faster than diffusion with active filaments January 11th, 2017

Discoveries

A big nano boost for solar cells: Kyoto University and Osaka Gas effort doubles current efficiencies January 21st, 2017

A toolkit for transformable materials: How to design materials with reprogrammable shape and function January 20th, 2017

Explaining how 2-D materials break at the atomic level January 20th, 2017

New research helps to meet the challenges of nanotechnology: Research helps to make the most of nanoscale catalytic effects for nanotechnology January 20th, 2017

Announcements

A big nano boost for solar cells: Kyoto University and Osaka Gas effort doubles current efficiencies January 21st, 2017

A toolkit for transformable materials: How to design materials with reprogrammable shape and function January 20th, 2017

New research helps to meet the challenges of nanotechnology: Research helps to make the most of nanoscale catalytic effects for nanotechnology January 20th, 2017

Ultra-precise chip-scale sensor detects unprecedentedly small changes at the nanoscale January 20th, 2017

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

A big nano boost for solar cells: Kyoto University and Osaka Gas effort doubles current efficiencies January 21st, 2017

A toolkit for transformable materials: How to design materials with reprogrammable shape and function January 20th, 2017

Explaining how 2-D materials break at the atomic level January 20th, 2017

New research helps to meet the challenges of nanotechnology: Research helps to make the most of nanoscale catalytic effects for nanotechnology January 20th, 2017

Tools

Chemists Cook up New Nanomaterial and Imaging Method: Nanomaterials can store all kinds of things, including energy, drugs and other cargo January 19th, 2017

Nanometrics to Announce Fourth Quarter and Full Year Financial Results on February 7, 2017 January 19th, 2017

Distinguishing truth under the surface: electrostatic or mechanic December 31st, 2016

Nanomechanics Inc. Continues Growth in Revenue and Market Penetration: Leading nanoindentation company reports continued growth in revenues and distribution channels on national and international scales December 27th, 2016

Nanobiotechnology

New research helps to meet the challenges of nanotechnology: Research helps to make the most of nanoscale catalytic effects for nanotechnology January 20th, 2017

Chemists Cook up New Nanomaterial and Imaging Method: Nanomaterials can store all kinds of things, including energy, drugs and other cargo January 19th, 2017

'5-D protein fingerprinting' could give insights into Alzheimer's, Parkinson's January 19th, 2017

Nanoscale Modifications can be used to Engineer Electrical Contacts for Nanodevices January 13th, 2017

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project