Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Novel Quantum Dot-Based Technique Sees 100 Different Molecules in a Single Cell

Abstract:
Better diagnosis and treatment of cancer could hinge on the ability to rapidly map out networks of dozens of molecules in individual tumor cells. New research from the University of Washington offers a more comprehensive way of analyzing a single cell's unique behavior and could reveal patterns that indicate why a cell will or will not become malignant.

Novel Quantum Dot-Based Technique Sees 100 Different Molecules in a Single Cell

Bethesda, MD | Posted on July 15th, 2013

Xiaohu Gua and graduate student Pavel Zrazhevskiy have used an array of distinctly colored quantum dots to illuminate 100 biomarkers, a ten-fold increase from the current research standard, to help analyze individual cells from cultures or tissue biopsies. The investigators published their findings in the journal Nature Communications.

While other approaches have measured multiple biomarkers in a single cell, what makes this technique so promising is that it reuses the same precious tissue sample in a cyclical process to measure 100 biomolecules in groups of ten. The process starts by pairing a commercially available antibody that is known to bind with specific biomolecule with a quantum dot of distinct size and therefore color. The investigators then inject a solution of ten of these antibody-quantum dot pairs onto a tissue sample and use a fluorescence microscope to quantify which of the constructs bind at the single cell level.

Once the measurement is complete, they then wash the tissue sample with a fluid of detergents at low pH to get rid of the antibodies and quantum dots without degrading the tissue sample. The two investigators have shown that they can repeat this process at least ten times without producing any signs of tissue damage.

The researchers note that because this methodology uses commercially available enzymes and standard fluorescence microscopes, it is relatively low cost. They also plan to automate the procedure using microfluidics and automated image processing technologies.

####

About The National Cancer Institute (NCI)
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Contacts:
National Cancer Institute
Office of Technology & Industrial Relations
ATTN: NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A49
31 Center Drive , MSC 2580
Bethesda , MD 20892-2580

Copyright © The National Cancer Institute (NCI)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

View open access full paper - “Quantum dot imaging platform for single-cell molecular profiling.”

Related News Press

News and information

MIG Takes a Roll-Up-Your-Sleeves Approach with Revamped MEMS/Sensors Technical Event -- MIG welcomes technologists to MEMS Technical Congress, emphasizes working groups and breakout sessions on emerging MEMS & sensors, tech transfer and integration March 6th, 2015

Phenom-World announces the Phenom XL, world’s fastest desktop SEM to handle large samples March 6th, 2015

Air Bearing Stage / Systems Introduced by PI at Photonics West March 6th, 2015

Consistent Scalable Functionalised Graphene Capacity March 5th, 2015

American Chemical Society Presidential Symposia: nanoscience, international chemistry March 5th, 2015

Imaging

Phenom-World announces the Phenom XL, world’s fastest desktop SEM to handle large samples March 6th, 2015

Govt.-Legislation/Regulation/Funding/Policy

New research could lead to more efficient electrical energy storage March 4th, 2015

Energy-generating cloth could replace batteries in wearable devices March 4th, 2015

The taming of magnetic vortices: Unified theory for skyrmion-materials March 3rd, 2015

Black phosphorus is new 'wonder material' for improving optical communication March 3rd, 2015

Nanomedicine

Patent for the Novel Cancer Therapies – Ceramide Nanoliposomes March 4th, 2015

Arrowhead to Present at 2015 Barclays Global Healthcare Conference March 4th, 2015

Democratizing synthetic biology: New method makes research cheaper, faster, and more accessible March 3rd, 2015

Pens filled with high-tech inks for do-it-yourself sensors March 3rd, 2015

Discoveries

Enhanced Graphene Components for Next Generation Racing Yacht March 5th, 2015

American Chemical Society Presidential Symposia: nanoscience, international chemistry March 5th, 2015

Strength in numbers: Researchers develop the first-ever quantum device that detects and corrects its own errors March 4th, 2015

New research could lead to more efficient electrical energy storage March 4th, 2015

Announcements

MIG Takes a Roll-Up-Your-Sleeves Approach with Revamped MEMS/Sensors Technical Event -- MIG welcomes technologists to MEMS Technical Congress, emphasizes working groups and breakout sessions on emerging MEMS & sensors, tech transfer and integration March 6th, 2015

Phenom-World announces the Phenom XL, world’s fastest desktop SEM to handle large samples March 6th, 2015

Air Bearing Stage / Systems Introduced by PI at Photonics West March 6th, 2015

Get ready for NanoDays! March 5th, 2015

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Strength in numbers: Researchers develop the first-ever quantum device that detects and corrects its own errors March 4th, 2015

Energy-generating cloth could replace batteries in wearable devices March 4th, 2015

Experiment and theory unite at last in debate over microbial nanowires: New model and experiments settle debate over metallic-like conductivity of microbial nanowires in bacterium March 4th, 2015

Magnetic vortices in nanodisks reveal information: Researchers from Dresden and Jülich use microwaves to read out information from smallest storage devices March 4th, 2015

Quantum Dots/Rods

Optical nanoantennas set the stage for a NEMS lab-on-a-chip revolution February 24th, 2015

QD Vision Named Edison Award Finalist for Innovative Color IQ™ Quantum Dot Technology February 23rd, 2015

Ocean Optics Names Winner of 2015 Young Investigator Award: Cash prize and grant awarded during SPIE BiOS/Photonics West 2015 conference February 21st, 2015

Rediscovering spontaneous light emission: Berkeley researchers develop optical antenna for LEDs February 3rd, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE