Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Oregon chemists moving forward with tool to detect hydrogen sulfide: Newly developed approach could benefit basic medical research and find H2S in the environment

Doctoral student Leticia A. Montoya and Michael Pluth, professor of chemistry, of the University of Oregon have developed a sensitive probe that detects H2S in biological samples and in the environment.

Credit: University of Oregon
Doctoral student Leticia A. Montoya and Michael Pluth, professor of chemistry, of the University of Oregon have developed a sensitive probe that detects H2S in biological samples and in the environment.

Credit: University of Oregon

Abstract:
University of Oregon chemists have developed a selective probe that detects hydrogen sulfide (H2S) levels as low as 190 nanomolar (10 parts per billion) in biological samples. They say the technique could serve as a new tool for basic biological research and as an enhanced detection system for H2S in suspected bacterially contaminated water sources.

Oregon chemists moving forward with tool to detect hydrogen sulfide: Newly developed approach could benefit basic medical research and find H2S in the environment

Eugene, OR | Posted on June 24th, 2013

Hydrogen sulfide, a colorless gas, has long been known for its dangerous toxicity -- and its telltale smell of rotten eggs -- in the environment, but in the last decade the gas has been found to be produced in mammals, including humans, with seemingly important roles in molecular signaling and cardiac health. Detection methods for biological systems are emerging from many laboratories as scientists seek to understand the roles of H2S in general health and different diseases.

Reporting in the Journal of Organic Chemistry -- online in advance of regular print publication -- researchers in the UO lab of Michael D. Pluth, professor of chemistry, describe the development of a colorimetric probe that relies on nucleophilic aromatic substitution to react selectively with H2S to produce a characteristic purple product, allowing for precise H2S measurement.

"This paper describes a new way to selectively detect H2S," said Pluth, who has been pursuing detection methods for the gas under a National Institutes of Health "Pathway to Independence" grant. That early career award began while he was a postdoctoral researcher at the Massachusetts Institute of Technology. "This technique allows you to use instruments to quantify how much H2S has been produced in a sample, and the distinctive color change allows for naked-eye detection."

In biological samples, he said, the approach allows for a precise measurement. In the environment, he added, the technique could be used to determine if potentially harmful H2S-producing bacteria are a contaminant in water sources through the creation of testing kits to detect the gas when levels are above a defined threshold.

The key to the technique, said the paper's lead author, doctoral student Leticia A. Montoya, is the reaction process in which the probe reacts with H2S to produce a distinctly identifiable purple compound. "This method allows you look selectively at hydrogen sulfide versus any other nucleophiles or biological thiols in a system," Montoya said. "It allows you to more easily visualize where H2S is present."

The chemical reaction produced in the experiments, Pluth said, also holds the potential to be applied in a variety of materials, on surfaces and films, with appropriate modifications. The UO has applied for a provisional patent to cover the technology.

The study is the second in which Pluth's lab has reported potential detection probes for H2S. Last year, in the journal Chemical Communications, Montoya and Pluth described their development of two bright fluorescent probes that sort out H2S from among cysteine, glutathione and other reactive sulfur, nitrogen and oxygen species in living cells.

"We're really interested in making sharper tools," Pluth said. "We have the basic science worked out, and now we want to move forward to fine-tune our tools so that we can better use them to answer important scientific questions."

"University of Oregon researchers are helping to foster a more sustainable future by developing powerful new tools and entrepreneurial technologies," said Kimberly Andrews Espy, vice president for research and innovation and dean of the UO graduate school. "This important research from Dr. Pluth's lab may someday alert us to environmental contaminants and could also impact basic science and human health."

Co-authors with Montoya and Pluth on the newly published paper were UO undergraduate students Taylor F. Pearce and Ryan J. Hansen, and Lev N. Zakharov of the UO-based Center for Advanced Materials Characterization in Oregon (CAMCOR). The NIH grant to Pluth (R00 GM092970) came from the National Institute for General Medical Sciences. The research also utilized UO-based nuclear magnetic resonance facilities that are supported by the National Science Foundation (ARRA CHE-0923589).

####

About University of Oregon
The University of Oregon is among the 108 institutions chosen from 4,633 U.S. universities for top-tier designation of "Very High Research Activity" in the 2010 Carnegie Classification of Institutions of Higher Education. The UO also is one of two Pacific Northwest members of the Association of American Universities.

For more information, please click here

Contacts:
Michael D. Pluth
assistant professor of chemistry
541-346-7477


Leticia A. Montoya
doctoral student, chemistry

Copyright © University of Oregon

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Pluth faculty page:

Pluth lab:

Department of Chemistry:

Follow UO Science on Facebook:

UO Science on Twitter:

More UO Science/Research News:

Related News Press

News and information

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Govt.-Legislation/Regulation/Funding/Policy

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

'Electronic skin' could improve early breast cancer detection October 29th, 2014

Nanomedicine

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Production of Biocompatible Polymers in Iran October 30th, 2014

Amorphous Coordination Polymer Particles as alternative to classical nanoplatforms for nanomedicine October 30th, 2014

'Electronic skin' could improve early breast cancer detection October 29th, 2014

Discoveries

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Announcements

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Water

Iranians Present Model to Predict Photocatalytic Process in Removal of Pollutants October 30th, 2014

Nanoparticles Display Ability to Improve Efficiency of Filters October 28th, 2014

Iranian, Malaysian Scientists Study Nanophotocatalysts for Water Purification October 23rd, 2014

New Nanocomposites Help Elimination of Toxic Dyes October 15th, 2014

Nanobiotechnology

Tiny carbon nanotube pores make big impact October 29th, 2014

Molecular beacons shine light on how cells 'crawl' October 27th, 2014

Breakthrough in molecular electronics paves the way for DNA-based computer circuits in the future: DNA-based programmable circuits could be more sophisticated, cheaper and simpler to make October 27th, 2014

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE