Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Single-Cell Transfection Tool Enables Added Control for Biological Studies: McCormick researchers develop method of delivering molecules into targeted cells

Transfection of the dextran Alexa Fluor 488 dye into a targeted HeLa cell using Nanofountain Probe Electroporation. Reprinted with permission from Nano Letters. Copyright 2013 American Chemical Society.
Transfection of the dextran Alexa Fluor 488 dye into a targeted HeLa cell using Nanofountain Probe Electroporation. Reprinted with permission from Nano Letters.

Copyright 2013 American Chemical Society.

Abstract:
Northwestern University researchers have developed a new method for delivering molecules into single, targeted cells through temporary holes in the cell surface. The technique could find applications in drug delivery, cell therapy, and related biological fields.

Single-Cell Transfection Tool Enables Added Control for Biological Studies: McCormick researchers develop method of delivering molecules into targeted cells

Evanston, IL | Posted on May 22nd, 2013

Bulk electroporation — a technique used to deliver molecules into cells through reversible nanopores in the cell membrane that are caused by exposing them to electric pulses — is an increasingly popular method of cell transfection. (Cell transfection is the introduction of molecules, such as nucleic acids or proteins, into a cell to change its properties.)

However, because bulk electroporation applies electric pulses to a bulk cell solution, it results in heterogeneous cell populations and often low cell viability. To solve these problems, Northwestern University researchers have developed a novel tool for single-cell transfection.

The new method, called nanofountain probe electroporation (NFP-E), allows researchers to deliver molecules into targeted cells through temporary nanopores in the cell membrane created by a localized electric field applied to a small portion of the cell. The method enables researchers to control dosage by varying the duration of the electric pulses, which provides unprecedented control of cell transfection.

"This is really exciting," said Horacio Espinosa, James and Nancy Farley Professor of Manufacturing and Entrepreneurship at Northwestern's McCormick School of Engineering and one of the paper's authors. "The ability to precisely deliver molecules into single cells is needed for biotechnology researchers to advance the state-of-the-art in therapeutics, diagnostics, and drug delivery toward the promise of personalized medicine."

A paper describing the research, "Nanofountain Probe Electroporation (NFP-E) of Single Cells," was published May 7 in the journal Nano Letters.

NFP-E is based on nanofountain probe (NFP) technology developed in Espinosa's lab. The NFP-E chip consists of an array of microfabricated cantilever probes with integrated microfluidic channels. The probe has previously been used for high-speed nanopatterning of proteins and nanoparticles for drug delivery studies.

The new single-cell transfection application couples the probe with an electrode and fluid control system that can be easily connected to a micromanipulator or atomic force microscope for position control. This integrated system allows the entire transfection process and post-transfection cell response to be monitored by an optical microscope.

The NFP-E system is being developed for commercialization by iNfinitesimal LLC, a Northwestern spin-off company founded by Espinosa, and is expected to be available in late 2013.

The technique is proving to be extremely robust and multi-functional. Researchers have used the NFP-E chip to transfect HeLa cells with polysaccharides, proteins, DNA hairpins, and plasmid DNA with single-cell selectivity, high transfection efficiency (up to 95%), qualitative dosage control, and very high viability (up to 92%).

In addition to Espinosa, authors of the research paper include Wonmo Kang, Fazel Yavari, Majid Minary-Jolandan, Juan P. Giraldo-Vela, Asmahan Safi, Rebecca McNaughton, and Victor Parpoil. The research was supported by the National Science Foundation and the National Institutes of Health.

####

For more information, please click here

Contacts:
Megan Fellman

847-491-3115

Copyright © Northwestern University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Download paper:

Related News Press

News and information

Relieving electric vehicle range anxiety with improved batteries: Lithium-sulfur batteries last longer with nanomaterial-packed cathode April 16th, 2014

Aerotech X-Y ball-screw stage for economical high performance Planar positioning April 16th, 2014

Energy Research Facility Construction Project at Brookhaven Lab Wins U.S. Energy Secretary's Achievement Award April 16th, 2014

Malvern reports on the publication of the 1000th peer-reviewed paper to cite NanoSight’s Nanoparticle Tracking Analysis, NTA April 16th, 2014

Govt.-Legislation/Regulation/Funding/Policy

Relieving electric vehicle range anxiety with improved batteries: Lithium-sulfur batteries last longer with nanomaterial-packed cathode April 16th, 2014

Energy Research Facility Construction Project at Brookhaven Lab Wins U.S. Energy Secretary's Achievement Award April 16th, 2014

Engineers develop new materials for hydrogen storage April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Nanomedicine

Nanobiotix Appoints Thierry Otin as Head of Manufacturing and Supply April 15th, 2014

PAM-XIAMEN Offers UV LED wafer April 15th, 2014

Nanocrystalline cellulose modified into an efficient viral inhibitor April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Discoveries

Nanocrystalline cellulose modified into an efficient viral inhibitor April 15th, 2014

Tiny particles could help verify goods: Chemical engineers hope smartphone-readable microparticles could crack down on counterfeiting April 15th, 2014

A molecular approach to solar power: Switchable material could harness the power of the sun — even when it’s not shining April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Announcements

Relieving electric vehicle range anxiety with improved batteries: Lithium-sulfur batteries last longer with nanomaterial-packed cathode April 16th, 2014

Aerotech X-Y ball-screw stage for economical high performance Planar positioning April 16th, 2014

Energy Research Facility Construction Project at Brookhaven Lab Wins U.S. Energy Secretary's Achievement Award April 16th, 2014

Malvern reports on the publication of the 1000th peer-reviewed paper to cite NanoSight’s Nanoparticle Tracking Analysis, NTA April 16th, 2014

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals

Relieving electric vehicle range anxiety with improved batteries: Lithium-sulfur batteries last longer with nanomaterial-packed cathode April 16th, 2014

Malvern reports on the publication of the 1000th peer-reviewed paper to cite NanoSight’s Nanoparticle Tracking Analysis, NTA April 16th, 2014

A molecular approach to solar power: Switchable material could harness the power of the sun — even when it’s not shining April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Nanobiotechnology

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone April 15th, 2014

In latest generation of tiny biosensors, size isn't everything: UCLA researchers overturn conventional wisdom on nanowire-based diagnostic devices April 11th, 2014

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE