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Agilent Technologies Inc. (NYSE: A) today announced the availability of a high-performance AFM/Raman system for life science and materials science applications. The system seamlessly integrates the Agilent 6000ILM AFM (atomic force microscope) and a HORIBA XploRA INV (inverted Raman microscope). The combination enables researchers to go beyond the optical diffraction limit to achieve nanoscale resolution as they perform Raman spectroscopy.
"Key capabilities of this new high-precision system include AFM/Raman co-localization, tip-enhanced Raman spectroscopy for transparent samples, and AFM force-volume spectroscopy," said Jeff Jones, general manager for Agilent's nanoinstrumentation facility in Chandler, Ariz. "It's an ideal solution for advanced life science research, including studies of cell membranes, the surface structure of cells, individual proteins, single molecules, and biopolymers, as well as for investigating novel materials such as graphene."
AFM/Raman co-localization can be performed either sequentially, using the new system's Raman laser scanning option, or simultaneously, using Agilent PicoView software to control the system. Raman mapping is achieved by acquiring a complete Raman spectrum at each pixel of a 2-D image to create a detailed chemical image of the sample. This image is generated by plotting the peak intensity (material concentration), peak position (molecular structure or material stress), or peak width (crystallinity). The system precisely overlays the detailed chemical image with a 3-D AFM topography image.
The system's XY piezo stage affords both Raman and AFM measurements. The 6000ILM AFM provides a wide range of direct sample property measurements (elasticity, for example) that can be correlated via Raman with chemical composition. Tip-enhanced Raman spectroscopy is also possible.
Agilent's new AFM/Raman system provides an extensive range of AFM force-volume spectroscopy capabilities. Results are fast and reliable. Researchers can use their own algorithms and plug-ins, select their own data points on the fly, acquire force-curve measurements on any data point, and change experimental parameters in real time.
Additional advantages include unrivaled in-liquid AFM imaging via Agilent's patented MAC Mode, an incubator perfusion cell sample plate to facilitate dynamic studies in liquids and gases, and a top-view video optics package that offers the ability to see opaque samples while scanning.
An image of the Agilent 6000ILM AFM and a HORIBA XploRA INV is located at www.agilent.com/find/6000ILMAFM_images .
AFM Instrumentation from Agilent
Agilent offers high-precision, modular AFM solutions for research, industry and education. Worldwide support is provided by experienced application scientists and technical service personnel. Agilent's leading-edge R&D laboratories are dedicated to the timely introduction and optimization of innovative and easy-to-use AFM technologies.
About Agilent Technologies Inc.
Agilent Technologies Inc. (NYSE: A) is the world’s premier measurement company and a technology leader in chemical analysis, life sciences, diagnostics, electronics, and communications. The company’s 20,500 employees serve customers in more than 100 countries. Agilent had revenues of $6.9 billion in fiscal 2012.
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Janet Smith, Americas
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Joan Horwitz, nanomeasurement
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