Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Illinois State University use Nanoparticle Tracking Analysis in their development of novel bioanalytical assays

PhD student, Andre James, from the Driskell group at Illinois State University uses the NanoSight NTA system
PhD student, Andre James, from the Driskell group at Illinois State University uses the NanoSight NTA system

Abstract:
NanoSight reports on how Nanoparticle Tracking Analysis, NTA, is being used in the development of novel bioanalytical assays at Illinois State University. The main application is the characterization of gold nanoparticles and differentiation between monodisperse samples from small numbers of aggregated materials.

Illinois State University use Nanoparticle Tracking Analysis in their development of novel bioanalytical assays

Salisbury, UK | Posted on March 12th, 2013

Speeding up the detection and identification of viruses is one of the areas of new research of Illinois State University Assistant Professor of Chemistry Jeremy Driskell. This has been recognised by the US Department of Defense in their recent award of a major grant. Reliable and accurate nanoparticle composition is important in such work.

Here, Dr Driskell describes how he has used various techniques.

Talking about his current work, Dr Driskell says "Our research group is focused on the development of novel bioanalytical assays which includes detection of nucleic acids, proteins, and whole viruses. While other groups aim to improve assay sensitivity or detection limits, our central focus is on reducing assay speed and complexity. We are currently using gold nanoparticles and gold filters to develop assays utilizing surface-enhanced Raman spectroscopy for detection. In the process of characterizing the gold nanoparticles and monitoring the modification steps required for the SERS assays, we found that particle sizing techniques such as Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) could also be used for assay readout. This finding and application is detailed in our recent publication in the Analyst." (1).

Dr Driskell continued: "We began by using DLS to characterize gold nanoparticles that we modify with Raman reporter molecules and antibodies. This was a simple means of detecting particle aggregation as a result of surface modification and is more sensitive than colorimetric detection, which we found was not useful for conditions that invoked slight aggregation. When we learned of NanoSight and NTA, we compared the data to DLS. For our purposes, NTA gave a much more accurate representation of the actual particle sizes in our solutions. DLS would frequently indicate aggregation of a nanoparticle population while NTA revealed few large aggregates while the majority of the particles remained monodisperse. Ultimately we concluded it was a better technique to give us a better understanding of nanoparticle composition (aggregates compared with individual particles) in terms of absolute numbers."

To find out about the company and to learn more about particle characterization using NanoSight's unique nanoparticle tracking analysis solutions, visit www.nanosight.com and register to receive the next issue of NanoTrail, the company's electronic newsletter.
(1)
"Monitoring gold nanoparticle conjugation and analysis of biomolecular binding with nanoparticle tracking analysis (NTA) and dynamic light scattering (DLS)" - A James & J Driskell, DOI: 10.1039/c2an36467k.

####

About NanoSight
NanoSight delivers the world's most versatile and proven multi-parameter nanoparticle analysis in a single instrument.

NanoSight's "Nanoparticle Tracking Analysis" (NTA) detects and visualizes populations of nanoparticles in liquids down to 10 nm, dependent on material, and measures the size of each particle from direct observations of diffusion. Additionally, NanoSight measures concentration and a fluorescence mode differentiates suitably-labelled particles within complex background suspensions. Zeta potential measurements are similarly particle-specific. It is this particle-by-particle methodology that takes NTA beyond traditional light scattering and other ensemble techniques in providing high-resolution particle size distributions and validates data with information-rich video files of the particles moving under Brownian motion.

This simultaneous multiparameter characterization matches the demands of complex biological systems, hence its wide application in development of drug delivery systems, of viral vaccines, and in nanotoxicology. This real-time data gives insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner. NanoSight has a growing role in biodiagnostics, being proven in detection and speciation of nanovesicles (exosomes) and microvesicles.

NanoSight has installed more than 500 systems worldwide with users including BASF, GlaxoSmithKline, Merck, Novartis, Pfizer, Proctor and Gamble, Roche and Unilever together with the most eminent universities and research institutes. NanoSight's technology is validated by 600+ third party papers citing NanoSight results. NanoSight's leadership position in nanoparticle characterization is consolidated further with publication of an ASTM International standard, ASTM E2834, which describes the NTA methodology for detection and analysis of nanoparticles.

For more information, please click here

Contacts:
NanoSight Limited
Minton Park
London Road
Amesbury SP4 7RT UK
T +44(0)1980 676060
F +44(0)1980 624703
www.nanosight.com


Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997
www.talking-science.com

Copyright © NanoSight

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Conductive Inks: booming to $2.8 billion by 2024 April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

INSCX™ exchange to present Exchange trade reporting mechanism for engineered nanomaterials (NMs) to UK regulation agencies, insurers and upstream/downstream users April 17th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Academic/Education

Director Wally Pfister joins UC Berkeley neuroengineers to discuss the science behind ‘Transcendence’ April 7th, 2014

First annual science week highlights STEM pipeline and partnerships: UB, SUNY Buffalo State and ECC team up with the City of Buffalo and its schools for April 7-11 events April 3rd, 2014

Global 450 consortium announces new general manager of internal operations: TSMC’s Cheng-Chung Chien Receives Unanimous Support, Brings History of Innovation and Efficiency to Global Consortium of Companies Driving Industry Transition to 450mm Wafer Technology March 26th, 2014

NanoTecNexus to Host "Chemistry of Wine" Fundraiser in Support of STEM Education - Collaborations Key to Success - March 20th, 2014

Nanomedicine

Novel stapled peptide nanoparticle combination prevents RSV infection, study finds April 17th, 2014

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

Harris & Harris Group Continues Its Blog Series to Highlight Most Impactful Portfolio Companies With Champions Oncology, Inc. April 17th, 2014

Announcements

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

INSCX™ exchange to present Exchange trade reporting mechanism for engineered nanomaterials (NMs) to UK regulation agencies, insurers and upstream/downstream users April 17th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Tools

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

Scientists Capture Ultrafast Snapshots of Light-Driven Superconductivity: X-rays reveal how rapidly vanishing 'charge stripes' may be behind laser-induced high-temperature superconductivity April 16th, 2014

Aerotech X-Y ball-screw stage for economical high performance Planar positioning April 16th, 2014

Malvern reports on the publication of the 1000th peer-reviewed paper to cite NanoSight’s Nanoparticle Tracking Analysis, NTA April 16th, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE