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Home > Press > Optimum Method for Application of Matrix Fluorescence Spectrometry in Nanobiotechnology Studies

Abstract:
In addition to introducing the advantages of matrix fluorescence spectrometry methods in nanobiotechnology studies, Iranian researchers succeeded in presenting new analysis methods for their optimum application in these fields.

Optimum Method for Application of Matrix Fluorescence Spectrometry in Nanobiotechnology Studies

Tehran, Iran | Posted on December 11th, 2012

In matrix fluorescence spectrometry methods, a matrix is allocated to each sample or each stage of the experiment instead of a number or a vector. In this research, the abovementioned issues were used in order to study the interaction between gold nanoparticles and DNA.

The application and DNA characterization and studies based on fluorescence techniques is growing due to their advantages such as high sensitivity and the possibility to carry out multiple analyses. Fluorescence methods are very important in nanobiotechnology researches, especially in the application of modified or non-modified gold nanoparticles in the study of biomolecules such as DNA and proteins. In fact, one of the main reasons for the rapid extension of the application of gold nanoparticles in biological studies is their optical properties, which is caused by surface plasmon resonance in these particles. The role of gold nanoparticles in studies based on fluorescence techniques is much more important as effective fluorescence reducing agents.

Among the characteristics of the research, mention can be made of the application of nanosciences in biotechnological studies and in the designing of more effective tools by using fluorescence spectrometry method. This research introduces and highlights the advantages of the use of multi-dimensional fluorescence spectrometry data in studying applications and in the designing of sensors to determine biological molecules. It also presents analysis tools in order to obtain the maximum amount of useful information from them.

Results of the research have been published on 7 August 2012 in Analytical Chemistry, vol. 84, issue 15, pp. 6603-6610.

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