Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Microfluidic Breakthroughs Offer New Options for Ease and Accuracy in Extraction of Rare Cells or Separating Blood

Photo By: Xiao Wang
This image shows the size-dependent extraction of particles. The fluorescent image indicates trapping of larger particles while smaller particles pass through.
Photo By: Xiao Wang

This image shows the size-dependent extraction of particles. The fluorescent image indicates trapping of larger particles while smaller particles pass through.

Abstract:
Using something called "inertial microfluidics," University of Cincinnati researchers are able to continuously and selectively collect rare cells, such as circulating tumor cells, based on their size vs. other biomarkers. This could reduce analysis time and increase selectivity while reducing reliance on antibody-based testing in clinical tests.

Microfluidic Breakthroughs Offer New Options for Ease and Accuracy in Extraction of Rare Cells or Separating Blood

Cincinnati, OH | Posted on October 26th, 2012

At the Sixteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS) to be held Oct. 28-Nov. 1, in Okinawa, Japan, University of Cincinnati researchers will present four papers, including one detailing improvements in rare cell isolation and one detailing improvements, in terms of cost and time, of common blood tests.

Ian Papautsky, associate professor in UC's School of Electronic and Computing Systems (SECS), part of the College of Engineering and Applied Science, and a UC team are leading these research efforts.

In a paper titled "Continuous Rare Cell Extraction Using Self-Releasing Vortex in an Inertial Microfluidic Device" by Papautsky and co-authors Xiao Wang, UC doctoral student, and Jian Zhou, research associate, a new concept for separation of rare cells, such as prostate cancer cells or circulating tumor cells, using microfluidics, is detailed.

"Last year we showed we can selectively isolate prostate cancer cells, but only by running small sample volumes one at a time. Now we show that we can do this continuously," Papautsky said. "This is exciting because it allows for an entire blood draw to be processed, in continuous matter, in a shorter period of time."

These blood draws can be used to identify tumor cells for diagnostic or prognostic purposes. "Our approach is based purely on size. It doesn't rely on antibodies, which is important because not all cancer cells express antigens. So, if the cancer cells are, let's say, larger than 20 microns, we'll extract them," he explained.

The most common approach for looking for these circulating tumor cells is via a system that uses a selection using antibodies to detect antigens. "We could also use our device to prepare samples for systems that use antibody-based selection." This combined approach could potentially help reduce occurrence of false positives while significantly increasing the accuracy of the antibody-based tests.

Another area in which this device could be useful is in working with cell cultures. "If you have a mixture of multiple cells where some cells are small and other cells are big, we could separate these cell populations very easily," Papautsky explained. "Anytime you need to separate based on size, we can do it using inertial microfluidics."

The advantage of inertial microfluidics in cell separation is that it can be done easily and without cumbersome equipment. This research is leading to an entirely new generation of testing capabilities which particularly lend themselves to direct use in the field and in physicians' offices in just about any country and any economic setting.

In another paper, titled "Sorting of Blood in Spiral Microchannels" Papautsky and doctoral student Nivedita Nivedita demonstrate continuous sorting of blood utilizing inertial microfluidics via a simple passive microfluidic device. Papautsky's lab has been developing the concept of using inertia to manipulate cells and particles during the last few years. "It's truly different and innovative because these microfluidic devices are really low cost while offering very high throughput," said Papautsky.

The device is, essentially, a clear, plastic, flexible square that is relatively small in size, at about a half an inch across, but big in concept. "With this particular device we can take a drop of blood, put it in the input port in the center, and separate," Papautsky explained. The device contains four outlet ports which separate the blood into different streams, allowing the collection of outputs containing dilute plasma, red blood cells and white blood cells.

The most common approach for looking for these circulating tumor cells is via a system that uses a selection using antibodies to detect antigens. "We could also use our device to prepare samples for systems that use antibody-based selection." This combined approach could potentially help reduce occurrence of false positives while significantly increasing the accuracy of the antibody-based tests.

Another area in which this device could be useful is in working with cell cultures. "If you have a mixture of multiple cells where some cells are small and other cells are big, we could separate these cell populations very easily," Papautsky explained. "Anytime you need to separate based on size, we can do it using inertial microfluidics."

The advantage of inertial microfluidics in cell separation is that it can be done easily and without cumbersome equipment. This research is leading to an entirely new generation of testing capabilities which particularly lend themselves to direct use in the field and in physicians' offices in just about any country and any economic setting.

In another paper, titled "Sorting of Blood in Spiral Microchannels" Papautsky and doctoral student Nivedita Nivedita demonstrate continuous sorting of blood utilizing inertial microfluidics via a simple passive microfluidic device. Papautsky's lab has been developing the concept of using inertia to manipulate cells and particles during the last few years. "It's truly different and innovative because these microfluidic devices are really low cost while offering very high throughput," said Papautsky.

The device is, essentially, a clear, plastic, flexible square that is relatively small in size, at about a half an inch across, but big in concept. "With this particular device we can take a drop of blood, put it in the input port in the center, and separate," Papautsky explained. The device contains four outlet ports which separate the blood into different streams, allowing the collection of outputs containing dilute plasma, red blood cells and white blood cells.
spiral microchannels

####

For more information, please click here

Contacts:
M.B. Reilly

513-556-1824

Copyright © University of Cincinnati

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Superfast light source made from artificial atom April 28th, 2016

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

JPK reports on the use of a NanoWizard AFM system at the University of Kaiserslautern to study the interaction of bacteria with microstructured surfaces April 28th, 2016

Microfluidics/Nanofluidics

POSTECH researchers develop a control algorithm for more accurate lab-on-a-chip devices April 6th, 2016

Microfluidic devices gently rotate small organisms and cells March 24th, 2016

New microwave imaging approach opens a nanoscale view on processes in liquids: Technique can explore technologically and medically important processes that occur at boundaries between liquids and solids, such as in batteries or along cell membranes March 16th, 2016

A portable device for rapid and highly sensitive diagnostics February 22nd, 2016

Nanomedicine

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

JPK reports on the use of a NanoWizard AFM system at the University of Kaiserslautern to study the interaction of bacteria with microstructured surfaces April 28th, 2016

Chemists use DNA to build the world's tiniest thermometer April 27th, 2016

Discoveries

Superfast light source made from artificial atom April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

Danish researchers behind vaccine breakthrough April 27th, 2016

NREL theory establishes a path to high-performance 2-D semiconductor devices April 27th, 2016

Announcements

Superfast light source made from artificial atom April 28th, 2016

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

JPK reports on the use of a NanoWizard AFM system at the University of Kaiserslautern to study the interaction of bacteria with microstructured surfaces April 28th, 2016

Events/Classes

Introducing the RE標ORK Bio-inspired Robotics Summit in Berlin April 27th, 2016

ORIG3N Added to Companies Presenting at Harris & Harris Group's Annual Meeting, Tuesday June 7, 2016, the New York Genome Center April 27th, 2016

Team builds first quantum cascade laser on silicon: Eliminates the need for an external light source for mid-infrared silicon photonic devices or photonic circuits April 21st, 2016

Arrowhead Pharmaceuticals Presents Preclinical Data on Renal Cell Carcinoma Program at AACR 2016 April 19th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic