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|The new Zetasizer Nano ZSP from Malvern Instruments delivers unique protein characterization capabilities in a single, easy to use instrument.|
The new Zetasizer Nano ZSP from Malvern Instruments is a high specification dynamic light scattering system that delivers unique protein characterization capabilities in a single, easy to use instrument. This top-of-the-range addition to Malvern's established Zetasizer Nano family enables highly sensitive measurement of zeta potential, for rapid, reliable measurement of the electrophoretic mobility of proteins, and subsequent calculation of the key parameter of protein charge. Measuring protein mobility (or zeta potential) by dynamic light scattering is faster and more convenient than conventional methods such as capillary electrophoresis and iso-electric focussing. Furthermore, the Zetasizer Nano ZSP requires only 20 microliters of sample and measures at concentrations down to 1 mg/mL.
Key to achieving superior protein mobility measurements are a highly sensitive measurement system, the ability to protect the sample from aggregation, and flexible software that presents data in an immediately usable format. The Zetasizer Nano ZSP has the count rate sensitivity needed to easily measure weakly scattering samples such as proteins, and the instrument's protein mobility measurement process is designed to maximize measurement quality using three specific features. Firstly, by combining size and zeta potential measurements the Zetasizer software can ensure that no aggregates are forming during the measurement, and warn the user is this does start to occur. Secondly, mobility measurements are performed in groups of sub-runs to allow extended periods of cooling, reducing the possibility of aggregation through heating. And thirdly, automatic optimization of measurement settings minimizes denaturation and aggregation during mobility measurements.
A suite of protein calculators has been added as part of the software developments that accompany the launch of the Zetasizer Nano ZSP, which are included with the instrument as standard. These include a dynamic DLS Debye Plot which allows calculation of the DLS interaction parameter, especially useful in protein formulation for biological therapeutics. There is also the ability to calculate protein charge from protein mobility, F(ka) from the Henry equation, interparticle distance, and thermodynamic diameter (virial radius).
Find out more at www.malvern.com/nano-zsp
Malvern, Malvern Instruments an Zetasizer are registered trademarks of Malvern Instruments Ltd
About Malvern Instruments
Malvern Instruments is a market leader in measuring performance controlling material properties. These include particle size, particle shape, zeta potential, molecular weight, size and conformation, rheological properties and chemical identification. Malvern delivers the systems, support and expertise that ensure the analytical integrity and productivity needed to drive research, development and manufacturing.
Malvern’s measurement solutions for scientists, technologists and engineers advance continually through customer collaboration. Complementary materials characterization systems deliver inter-related measurements that reflect the complexities of particulates and disperse systems, nanomaterials and macromolecules. Combining intelligently implemented technologies with in-depth industry applications knowledge and support, Malvern provides customers with the competitive advantage they demand.
Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Japan and Korea, a joint venture in India, a global distributor network and applications laboratories around the world.
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