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Iranian researchers from Shiraz University succeeded in the identification of presence or absence of any of the spot jumps in DNA sequence by designing a method that enables the distinction of all eight possible forms of single nucleotide polymorphism (SNP).
One of the important aspects of the study is that identification of the presence or absence of any of the spot jumps in DNA sequence took place in one step with significant sensitivity.
Taking into consideration the existing information, no method for the one-step identification of any of the possible spot jumps has so far been presented, and the presented methods need at least four tests in order to identify any of the eight possible spot jumps. Therefore, the method presented by the Iranian researchers facilitates the identification of SNP.
The research has been carried out in order to design a biosensor for the identification of any of the eight possible SNPs by using only one voltammogram. However, the proposed method has high potential to help the production of a biosensor in order to diagnose cancer. The biosensor is able to diagnose cancer cells when the nanoparticle-containing protein is attached to an appropriate receiver of cancer biomarkers.
The results of the research have been published in August-September 2012 in Biosensors and Bioelectronics, vol. 37, issue 1, pp. 11-18.
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