Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Ultra low level biodetection achieved by Dublin researchers on standard qNano nanopore instrument

Abstract:
Researchers at the Lee Bionanosciences Laboratory at UCD in Dublin have demonstrated the detection and measurement of biological analytes down to femtomolar concentration levels using an off the shelf qNano instrument. This ultra low level biodetection capability has implications for biomedical research and clinical development as trace amounts of a biological substance in a sample can now be detected and quantified using standard commercially available equipment.

Ultra low level biodetection achieved by Dublin researchers on standard qNano nanopore instrument

Dublin, Ireland and Christchurch, New Zealand | Posted on July 18th, 2012

The findings by Dr. Mark Platt (Loughborough University and formerly University College Dublin), Professor Gil Lee, (University College Dublin) and Dr. Geoff Willmott (MacDiarmid Institute, New Zealand) have been published in Small, the peer-reviewed journal on micro- and nanoscales science.

Platt and colleagues' method for femtomolar-level detection uses magnetic particle systems and can be applied to any biological particle or protein for which specific antibodies or aptamers exist. Resistive pulse sensing, the underlying technology of the qNano, was used to monitor individual and aggregated rod-shaped nanoparticles as they move through tunable pores in elastomeric membranes.

The authors say, "The strength of using the qNano is its simplicity and the ability to interrogate individual particles through a nanopore. This allowed us to establish a very sensitive measurement of concentration because we could detect the interactions occurring down to individual particle level."

The unique and technically innovative approach of the authors was to detect a molecule's presence by a process that results in end on end or side by side aggregation of rod shaped nickel-gold particles. The rods were designed so that any specific aptamer could be attached to one end only.

"By comparing particles of similar dimensions we demonstrated that the resistive pulse signal is fundamentally different for rod and sphere-shaped particles, and for rod shaped particles of different lengths. We could exploit these differences in a new agglutination assay to achieve these low detection levels," says Dr. Platt.

In the agglutination assay particles with a particular aspect ratio can be distinguished by two measurements: the measured drop in current as particles traverse the pore ( ?ip ), which reveals the particle's size, and the full width at half maximum (FWHM) duration of the resistive pulse, which relates to the particle's speed and length. Limits of detection down to femtomolar levels were thus able to be demonstrated.

The article "Resistive Pulse Sensing of Analyte-Induced Multicomponent Rod Aggregation Using Tunable Pores" Platt, M., Willmott, G. R. and Lee, G. U. (2012), Small. doi: 10.1002/smll.201200058 is online for subscribers at http://onlinelibrary.wiley.com/doi/10.1002/smll.201200058/abstract.

"This is a real milestone for Izon's technology as being able to measure biomolecules down to these extremely low levels opens up new bio-analysis options for researchers. 10 femtomolar was achieved, which is the equivalent dilution to 1 gram in 3.3 billion litres, or 1 gram in 1300 Olympic sized swimming pools," says Hans van der Voorn, Executive Chairman of Izon Science.

Izon Science will continue to work with Dr. Platt at Loughborough, and with University College Dublin and various customers to develop a series of diagnostic kits that can be used with the qNano to identify and measure biomolecules, viruses, and microvesicles.

"We're now developing standardised diagnostics kits for researchers which will allow them to optimise protocols for their particular targets of interest. The interest is in accurate quantification as much as the core detection," says van der Voorn.

Izon Science is the developer of the portable qNano and qViro-X instruments with unique size-tunable nanopores. The multi-parameter instruments offer significant accuracy and reliability improvements over light based techniques and are advancing research in a number of fields including nanomedicine, hematology, gene therapy and vaccine development.

Funding Sources:

Science Foundation of Ireland Research Grants 08/IN/1B0272 and 08/PR1/B1376, European Research Commision FP7-PEOPLE-2009-IEF-252935, New Zealand Ministry of Science and Innovation NERF Contract C08x0806, and The Royal Society of New Zealand's International Mobility Fund IMF10-B41.

####

About Izon Science
Izon Science has developed the world's first nanopore based measurement system available for general use. Izon's instruments are used for precise measurement and analysis of individual particles across a wide range of scientific fields including, nanomedicine, vaccinology, gene therapy and hematology. Izon originated in New Zealand and now sells its products in 30 countries. It has its European headquarters in Oxford, UK and US headquarters in Cambridge, MA. Website: www.izon.com; Facebook: www.facebook.com/izonscience; Twitter: www.twitter.com/izonscience


About the Lee Bionanosciences Lab at UCD:

Led by Professor Gil Lee, this interdisciplinary research group works at the interface between physics, chemistry and biology. Its current research areas broadly include:
- Synthesis of bionanomaterials with emphasis on assemblies of superparamagnetic nanoparticles
- Study of forces within and between single molecules and cells
- Development of highly sensitive, multiplexed biosensors based on magnetic agglutination
- Development of novel materials for down stream processing

For more information, please click here

Contacts:
Dr Mark Platt
Lecturer
Department of Chemistry
Lougborough University
Phone: +49 1509 222 573
Email:

Prof. Gil Lee
Bionanoscience Group
University College Dulbin
Phone: +353-1-716-2399


Hans van der Voorn
Executive Chairman
Izon Science
Phone: + 64 21 463 399


Sandra Lukey
Shine Group (PR for Izon Science)
Phone: + 64 21 2262 858

Copyright © Izon Science

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Lifeboat Foundation Responds to Largest Ebola Outbreak in History October 2nd, 2014

Iran's Sharif University to Host 4th Conference on Nanostructured Solar Cells October 2nd, 2014

Multifunctional Cotton Fabrics Produced in Iran Using Nanotechnology October 2nd, 2014

Graphene chips are close to significant commercialization October 1st, 2014

Production of Filters for Separation of Water from Petroleum Products in Iran October 1st, 2014

Imaging

Novel approach to magnetic measurements atom-by-atom October 1st, 2014

Stressed Out: Research Sheds New Light on Why Rechargeable Batteries Fail October 1st, 2014

Nanomedicine

Arrowhead Expands Management Team with Appointment of Susan Boynton as Vice President Global Regulatory Affairs October 1st, 2014

Nanobotmodels present metastasis and angiogenesis medical animation October 1st, 2014

'Stealth' nanoparticles could improve cancer vaccines October 1st, 2014

New Absorber Will Lead to Better Biosensor: Biosensors are more sensitive and able to detect smaller changes in the environment October 1st, 2014

Discoveries

Multifunctional Cotton Fabrics Produced in Iran Using Nanotechnology October 2nd, 2014

Platinum meets its match in quantum dots from coal: Rice University's cheap hybrid outperforms rare metal as fuel-cell catalyst October 1st, 2014

Stressed Out: Research Sheds New Light on Why Rechargeable Batteries Fail October 1st, 2014

New Absorber Will Lead to Better Biosensor: Biosensors are more sensitive and able to detect smaller changes in the environment October 1st, 2014

Announcements

Lifeboat Foundation Responds to Largest Ebola Outbreak in History October 2nd, 2014

Iran's Sharif University to Host 4th Conference on Nanostructured Solar Cells October 2nd, 2014

Multifunctional Cotton Fabrics Produced in Iran Using Nanotechnology October 2nd, 2014

Graphene chips are close to significant commercialization October 1st, 2014

Tools

Breakthrough in ALD-graphene by Picosun technology October 1st, 2014

Novel approach to magnetic measurements atom-by-atom October 1st, 2014

Stressed Out: Research Sheds New Light on Why Rechargeable Batteries Fail October 1st, 2014

Yale University and Leica Microsystems Partner to Establish Microscopy Center of Excellence: Yale Welcomes Scientists to Participate in Core Facility Opening and Super- Resolution Workshops October 20 Through 31, 2014 September 30th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE