Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Organic Nanotube Gels to Restore the Activity of Denatured Proteins - Restores the native structure of protein and protects it from heat and chemicals -

Figure 1 : Refolding of a denatured protein to create a native protein using a nanochannel in the organic nanotube gel
Figure 1 : Refolding of a denatured protein to create a native protein using a nanochannel in the organic nanotube gel

Abstract:


- Nanochannels in organic nanotubes act as space for restoration of the activity of denatured proteins.
- The organic nanotube can protect the restored protein from heat and chemicals.
- Efficient preparation of high-purity proteins and applications in nanoreactors and enzyme sensors are expected.

Organic Nanotube Gels to Restore the Activity of Denatured Proteins - Restores the native structure of protein and protects it from heat and chemicals -

Tokyo, Japan | Posted on July 12th, 2012

Naohiro Kameta (Researcher), Organic Nanotube Material Team, the Nanotube Research Center (Director: Sumio Iijima) of the National Institute of Advanced Industrial Science and Technology (AIST; President: Tamotsu Nomakuchi), has developed organic nanotube gels that restore the activity of denatured proteins by folding them into the native three-dimensional structure (refolding). The organic nanotube gels can protect proteins from heat and chemicals.

This technology has been realized by forming organic nanotube gels in which the inner and outer surface structures and diameter of the nanochannel are precisely controlled for the protein of interest. The denatured proteins are encapsulated in the process of the organic nanotube gel formation and are then recovered from the gel by using a change in pH. In this way, only proteins with restored activity can be obtained at high purity. Use of the organic nanotubes as the gel media has the benefit of allowing easy removal of the denaturant by water washing and easy separation and recovery of the protein of interest; these can be achieved without filtration or centrifugal separation. In addition, when proteins are encapsulated in the nanochannel of the organic nanotube, the activity of the proteins is not inactivated by heating or by adding a high concentration of denaturant. Expected applications of this technology include efficient preparation of high-purity proteins and use in nanoreactors and enzyme sensors by combining an enzyme with the organic nanotube.

Details of the results will be published online in ACS Nano, a scientific journal of the American Chemical Society, on May 23, 2012 (JST).

Social Background of Research

From a green innovation perspective, enzymes that promote chemical reactions in the body in a specific and highly selective manner with a high yield are attracting substantial attention as energy-efficient, low-environmental-impact catalysts in chemical industrial processes. An enzyme, which is a protein, is folded into a specific three-dimensional structure by the interaction of amino acids (protein components) and exhibits catalytic activity that is based on the structure. Recombinant technology using Escherichia coli is commonly used in the industrial production of proteins. However, an enzyme considerably changes its structure from the native three-dimensional one in the expression of a recombinant protein and aggregates of denatured proteins with no catalytic activity are formed. The restoring efficiency of proteins with a native three-dimensional structure and original catalytic activity from the aggregate is very low. Consequently, various additives to help suppress protein aggregation or to encourage protein refolding into the native three-dimensional structure have been developed. However, the additives have low yield and versatility.

In the body, there is a protein group called molecular chaperones that have nanospaces to encapsulate denatured proteins for isolation and to help with protein refolding. In recent years, porous inorganic materials and polymer nanoparticles that can mimic molecular chaperones have attracted attention. However, an additive is required for desorption of proteins from the porous inorganic materials or polymer nanoparticles and both the additive and incompletely refolded proteins contaminate the aimed protein. Therefore, a complex separation process is required and such a process may cause redenaturation of the protein.
History of Research

Over more than 10 years, AIST has been working to develop fibrous organic nanomaterials and tubular ones (i.e. organic nanotubes) formed by self-organization in solvents of amphiphilic molecules synthesized from renewable, natural products, such as sugars, amino acids, nucleic acids, and fatty acids. In recent years, AIST has established a process for mass production of organic nanotubes and has been developing various applications of these organic nanotubes.

The outer surface of conventional bilayer-membrane organic nanotubes has the same structure as the inner surface on the nanochannel side. Recently, by using molecular design and molecular arrangement control, AIST has created monolayer-membrane organic nanotubes with an inner-surface structure that differs from that of the outer surface. Providing the nanochannel-side surface with a structure that allows interaction with drugs, protein, DNA, and nanoparticles (called "guests"), the nanotube can encapsulate guests efficiently and selectively. Storage and release of the guests can be regulated by externally controlling the interaction.

The researcher formed organic nanotube gels in which the surface structure and the diameter of the nanochannel were precisely controlled to enable denatured protein molecules to be encapsulated as guests. He also aimed to develop new functions, such as the promotion of protein refolding and the protection of protein activity.

Future Plans

The researcher intends to create organic nanotube gels in which the surface structure and nanochannel diameter are controlled appropriately for a variety of proteins with different properties. He aims to develop an artificial molecular chaperone system through collaborative research, including provision of samples. He will also apply enzyme-conjugated organic nanotubes to nanoreactors and enzyme sensors.

####

About AIST
The National Institute of Advanced Industrial Science and Technology (AIST), led by President Nomakuchi, is a public research institution funded by the Japanese government to a large extent. The present AIST is a rather new research organization established in 2001. However, AIST and its predecessor organizations have been contributing to society through continuous advancement in technologies and support of Japanese industries since 1882.

For more information, please click here

Copyright © AIST

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Details of Research:

Related News Press

News and information

CTI Materials drives nano commercialization with it's patented surfactant free nanoparticle dispersions August 15th, 2018

Flipping the switch on supramolecular electronics August 14th, 2018

New technology can detect hundreds of proteins in a single sample: Improvement of barcoding technique offers cost-effective alternative to current technology August 13th, 2018

Amazingly 'green' synthesis method for high-tech dyes: Dyes that are also of great interest for organic electronics have recently been prepared and crystallised at TU Wien. All that is required is just water, albeit under highly unusual conditions. August 10th, 2018

Nanotubes/Buckyballs/Fullerenes/Nanorods

Nanotube 'rebar' makes graphene twice as tough: Rice University scientists test material that shows promise for flexible electronics August 3rd, 2018

Carbon is the new black: Researchers use carbon nanotubes to develop clothing that can double as batteries July 10th, 2018

Nano-saturn: Supramolecular complex formation: Anthracene macrocycle and C60 fullerene June 8th, 2018

Unzipping graphene nanotubes into nanoribbons: New study shows elegant mathematical solution to understand how the flow of electrons changes when carbon nanotubes turn into zigzag nanoribbons June 6th, 2018

Nanomedicine

New technology can detect hundreds of proteins in a single sample: Improvement of barcoding technique offers cost-effective alternative to current technology August 13th, 2018

Scientists squeeze nanocrystals in a liquid droplet into a solid-like state and back again: Simple chemical technique transforms crystal mixture where 2 liquids meet August 9th, 2018

Nanoscience and the future of healthcare kick off first day of ACS national meeting in Boston: Presidential events highlight safety, diversity and groundbreaking research August 2nd, 2018

Nano-kirigami: 'Paper-cut' provides model for 3D intelligent nanofabrication July 13th, 2018

Sensors

CTI Materials drives nano commercialization with it's patented surfactant free nanoparticle dispersions August 15th, 2018

Optical fibers that can 'feel' the materials around them August 7th, 2018

A molecular switch at the edge of graphene July 27th, 2018

Leti & Partners Launch Pilot Program to Assess New Perception Sensors for Autonomous Vehicles July 5th, 2018

Discoveries

How hot is Schrödinger's coffee? August 15th, 2018

Flipping the switch on supramolecular electronics August 14th, 2018

New technology can detect hundreds of proteins in a single sample: Improvement of barcoding technique offers cost-effective alternative to current technology August 13th, 2018

Breaking down the Wiedemann-Franz law: In a study exploring the coupling between heat and particle currents in a gas of strongly interacting atoms, physicists at ETH Zurich find puzzling behaviours August 10th, 2018

Announcements

How hot is Schrödinger's coffee? August 15th, 2018

CTI Materials drives nano commercialization with it's patented surfactant free nanoparticle dispersions August 15th, 2018

Flipping the switch on supramolecular electronics August 14th, 2018

New technology can detect hundreds of proteins in a single sample: Improvement of barcoding technique offers cost-effective alternative to current technology August 13th, 2018

Nanobiotechnology

New technology can detect hundreds of proteins in a single sample: Improvement of barcoding technique offers cost-effective alternative to current technology August 13th, 2018

Scientists squeeze nanocrystals in a liquid droplet into a solid-like state and back again: Simple chemical technique transforms crystal mixture where 2 liquids meet August 9th, 2018

Nanoscience and the future of healthcare kick off first day of ACS national meeting in Boston: Presidential events highlight safety, diversity and groundbreaking research August 2nd, 2018

UMBC researchers develop nanoparticles to reduce internal bleeding caused by blast trauma July 13th, 2018

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project