Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > The University of Colorado at Boulder uses Nanoparticle Tracking Analysis to characterize microvesicles as potential biomarkers

Abstract:
NanoSight, leading manufacturers of unique nanoparticle characterization technology, reports on the work of Professor Hang (Hubert) Yin's group at the University of Colorado at Boulder where they apply Nanoparticle Tracking Analysis (NTA) to characterize biological nanoparticles such as microvesicles.

The University of Colorado at Boulder uses Nanoparticle Tracking Analysis to characterize microvesicles as potential biomarkers

Salisbury, UK | Posted on June 19th, 2012

The Yin Research Lab is interested in studying at the interface of chemistry, Biology and engineering with a particular focus on structure-based drug design, cell signaling, biochemistry, biotechnology development and membrane protein simulations.

The main research goal of the group is to identify and design peptides that sense membrane curvature to better understand protein/peptide-lipid interactions and potentially create non-invasive probes to detect highly curved extracellular vesicles. Currently, we are studying microvesicles as potential biomarkers of tumor progression and cancer metastasis. These nanoparticles are shed into bodily fluids targeting other cells in the body and are vital for inter-cellular communication.

Their experimental protocol involves lipid vesicle preparation by pressure-controlled extrusion through different membrane pore sizes. Different lipid vesicle sizes are prepared in order to mimic the size range of the microvesicles that are shed into the extracellular matrix. Following vesicle extrusion, it is important to validate the vesicle size. By using Nanoparticle Tracking Analysis (NTA) technology, the results provide an accurate quantification of different populations of vesicle sizes present in the sample.

Prior to NTA, the group mostly used dynamic light scattering (DLS) to determine the sizes of our synthetic lipid vesicles. Speaking on their use of NTA, Professor Yin says "NTA brought several benefits over existing methods. The detection ranges from 10 - 2000 nm for vesicle sizes, dimensions that cover our liposome size of interest. Flow cytometry has a lower limit detection of ~200 nm to accurately measure particle sizes so did not reach our lower requirement while DLS measures the average size of all the particles present in the sample rather than accurately distinguish different pools of vesicle sizes, often creating a bias towards larger particles."

The group has recently published a paper in the Journal of Visualized Experimentation that used the NTA technology entitled Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Liposomes (Leslie A. Morton, Jonel P. Saludes, Hang Yin). To learn more, please refer to doi: 10.3791/4151.

To find out about the company and to learn more about particle characterization using NanoSight's unique nanoparticle tracking analysis solutions, visit www.nanosight.com and register to receive the next issue of NanoTrail, the company's electronic newsletter.

####

About NanoSight
NanoSight delivers the world's most versatile and proven multi-parameter nanoparticle analysis in a single instrument.

NanoSight's "Nanoparticle Tracking Analysis" (NTA) detects and visualizes populations of nanoparticles in liquids down to 10 nm, dependent on material, and measures the size of each particle from direct observations of diffusion. Additionally, NanoSight measures concentration and a fluorescence mode differentiates suitably-labelled particles within complex background suspensions. Zeta potential measurements assess the surface charge on particles. NTA's particle-by-particle methodology goes beyond traditional light scattering and other ensemble techniques in providing high-resolution particle size distributions and validates data with information-rich video files of the particles moving under Brownian motion.

NanoSight's simultaneous multiparameter characterization matches the demands of complex biological systems, hence its wide application in development of drug delivery systems, of viral vaccines, and in nanotoxicology. This real-time data gives insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner. NanoSight has a growing role in biodiagnostics, being proven in detection and speciation of nanovesicles (exosomes) and microvesicles.

NanoSight has installed more than 450 systems worldwide with users including BASF, GlaxoSmithKline, Merck, Novartis, Pfizer, Proctor and Gamble, Roche and Unilever together with the most eminent universities and research institutes. NanoSight's technology is validated by 400+ third party papers citing NanoSight results, consolidating NanoSight's leadership position in nanoparticle characterization.

For more information, please click here

Contacts:
NanoSight Limited
Minton Park
London Road
Amesbury SP4 7RT UK
T +44(0)1980 676060
F +44(0)1980 624703
www.nanosight.com


Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997
www.talking-science.com

Copyright © NanoSight

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

'Nano-hashtags' could provide definite proof of Majorana particles: Eindhoven network of nanowires gives particles the space to exchange places August 23rd, 2017

DNA sensor system developed for specific and sensitive measurement of cancer-relevant enzyme activity August 23rd, 2017

Lego proteins revealed: Self-assembling protein complexes based on a single mutation could provide scaffolding for nanostructures August 23rd, 2017

Heating quantum matter: A novel view on topology: Physicists demonstrate how heating up a quantum system can be used as a universal probe for exotic states of matter August 22nd, 2017

Imaging

Tokai University research: Nanomaterial wrap for improved tissue imaging August 21st, 2017

Thermo Fisher Scientific Advances Cryo-EM Leadership to Drive Structural Biology Discoveries: New Thermo Scientific Krios G3i raises bar for performance, automation and time-to-results Breakthrough Thermo Scientific Glacios provides a cryo-EM entry path for a broader range of res August 8th, 2017

New Quattro Field Emission ESEM Emphasizes Versatility and Ease of Use: Thermo Scientific Quattro ESEM allows materials science researchers to study nanoscale structure in almost any material under a range of environmental conditions August 8th, 2017

Thermo Fisher Scientific’s New Talos F200i S/TEM Delivers Flexible, High-Performance Imaging: New compact S/TEM can be configured to meet specific imaging and analytical requirements for materials characterization in research laboratories August 8th, 2017

Academic/Education

Two Scientists Receive Grants to Develop New Materials: Chad Mirkin and Monica Olvera de la Cruz recognized by Sherman Fairchild Foundation August 16th, 2017

Moving at the Speed of Light: University of Arizona selected for high-impact, industrial demonstration of new integrated photonic cryogenic datalink for focal plane arrays: Program is major milestone for AIM Photonics August 10th, 2017

Graduate Students from Across the Country Attend Hands-on NanoCamp: Prominent scientists Warren Oliver, Ph.D., and George Pharr, Ph.D., presented a weeklong NanoCamp for hand-picked graduate students across the United States July 26th, 2017

The Physics Department of Imperial College, London, uses the Quorum Q150T to deposit metals and ITO to make plasmonic sensors and electric contact pads July 13th, 2017

Nanomedicine

DNA sensor system developed for specific and sensitive measurement of cancer-relevant enzyme activity August 23rd, 2017

Lego proteins revealed: Self-assembling protein complexes based on a single mutation could provide scaffolding for nanostructures August 23rd, 2017

Tokai University research: Nanomaterial wrap for improved tissue imaging August 21st, 2017

Gold nanostars and immunotherapy vaccinate mice against cancer: New treatment cures, vaccinates mouse in small proof-of-concept study August 18th, 2017

Announcements

'Nano-hashtags' could provide definite proof of Majorana particles: Eindhoven network of nanowires gives particles the space to exchange places August 23rd, 2017

DNA sensor system developed for specific and sensitive measurement of cancer-relevant enzyme activity August 23rd, 2017

Lego proteins revealed: Self-assembling protein complexes based on a single mutation could provide scaffolding for nanostructures August 23rd, 2017

Heating quantum matter: A novel view on topology: Physicists demonstrate how heating up a quantum system can be used as a universal probe for exotic states of matter August 22nd, 2017

Tools

Nagoya physicists resolve long-standing mystery of structure-less transition: Nagoya University-led team of physicists use a synchrotron radiation X-ray source to probe a so-called 'structure-less' transition and develop a new understanding of molecular conductors August 21st, 2017

Tokai University research: Nanomaterial wrap for improved tissue imaging August 21st, 2017

Scientists from the University of Manchester and Diamond Light Source work with Deben to develop and test a new compression stage to study irradiated graphite at elevated temperatures August 15th, 2017

FRITSCH • Milling and Sizing! Innovations at POWTECH 2017 - Hall 2 • Stand 227 August 9th, 2017

Nanobiotechnology

DNA sensor system developed for specific and sensitive measurement of cancer-relevant enzyme activity August 23rd, 2017

Lego proteins revealed: Self-assembling protein complexes based on a single mutation could provide scaffolding for nanostructures August 23rd, 2017

Tokai University research: Nanomaterial wrap for improved tissue imaging August 21st, 2017

Gold nanostars and immunotherapy vaccinate mice against cancer: New treatment cures, vaccinates mouse in small proof-of-concept study August 18th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project