Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > DNA origami puts a smart lid on solid-state nanopore sensors: 2 TUM advances combine for new capabilities in single-molecule sensing

This illustration shows how a DNA origami nanoplate with a central aperture can serve as a smart lid or "gatekeeper" for a solid-state nanopore sensor. Researchers at the Technische Universitaet Muenchen have demonstrated that this arrangement can be used to filter biomolecules by size or to "fish" for specific target molecules by placing single-strand DNA receptors inside the aperture as "bait." With further research, they suggest, it might be possible to use such single-molecule sensors as the basis of a novel DNA sequencing system.

Credit: TU Muenchen
This illustration shows how a DNA origami nanoplate with a central aperture can serve as a smart lid or "gatekeeper" for a solid-state nanopore sensor. Researchers at the Technische Universitaet Muenchen have demonstrated that this arrangement can be used to filter biomolecules by size or to "fish" for specific target molecules by placing single-strand DNA receptors inside the aperture as "bait." With further research, they suggest, it might be possible to use such single-molecule sensors as the basis of a novel DNA sequencing system.

Credit: TU Muenchen

Abstract:
The latest advance in solid-state nanopore sensors - devices that are made with standard tools of the semiconductor industry yet can offer single-molecule sensitivity for label-free protein screening - expands their bag of tricks through bionanotechnology. Researchers at the Technische Universitaet Muenchen have enhanced the capabilities of solid-state nanopores by fitting them with cover plates made of DNA. These nanoscale cover plates, with central apertures tailored to various "gatekeeper" functions, are formed by so-called DNA origami - the art of programming strands of DNA to fold into custom-designed structures with specified chemical properties. The results are published in Angewandte Chemie International Edition.

DNA origami puts a smart lid on solid-state nanopore sensors: 2 TUM advances combine for new capabilities in single-molecule sensing

Munich, Germany | Posted on April 20th, 2012

Over the past few years, Prof. Hendrik Dietz's research group at TUM has been refining control over DNA origami techniques and demonstrating how structures made in this way can enable scientific investigations in diverse fields. Meanwhile, Dr. Ulrich Rant's research group has been doing the same for solid-state nanopore sensors, where the basic working principle is to urge biomolecules of interest, one at a time, through a nanometer-scale hole in a thin slab of semiconductor material. When biomolecules pass through or linger in such a sensor, minute changes in electrical current flowing through the nanopore translate into information about their identity and physical properties. Now Dietz and Rant, who are both Fellows of the TUM Institute for Advanced Study, have begun to explore what these two technologies can accomplish together.

The new device concept - purely hypothetical before this series of experiments - begins with the placement of a DNA origami "nanoplate" over the narrow end of a conically tapered solid-state nanopore. "Tuning" the size of the central aperture in the DNA nanoplate should allow filtering of molecules by size. A further refinement, placing single-stranded DNA receptors in the aperture as "bait," should allow sequence-specific detection of "prey" molecules. Conceivable applications include biomolecular interaction screens and detection of DNA sequences. In principle, such a device could even serve as the basis of a novel DNA sequencing system.

Step by step, the researchers investigated each of these ideas. They were able to confirm the self-assembly of custom-designed DNA origami nanoplates, and then their placement - after being electrically guided into position - over solid-state nanopores. They were able to demonstrate both size-based filtering of biomolecules and bait/prey detection of specific target molecules. "We're especially excited about the selective potential of the bait/prey approach to single-molecule sensing," Dietz says, "because many different chemical components beyond DNA could be attached to the appropriate site on a DNA nanoplate."

High-resolution sensing applications such as DNA sequencing will face some additional hurdles, however, as Rant explains: "By design, the nanopores and their DNA origami gatekeepers allow small ions to pass through. For some conceivable applications, that becomes an unwanted leakage current that would have to be reduced, along with the magnitude of current fluctuations."

This research was supported by the German Excellence Initiative through the TUM Institute for Advanced Study, the Nano Initiative Munich, and the Center for Integrated Protein Science Munich; by the Collaborative Research Center SFB 863 of the German Research Foundation (DFG); and by a European Research Council Starting Grant to Hendrik Dietz. Ruoshan Wei was supported by the TUM Graduate School's Faculty Graduate Center of Physics.

Original publication:
DNA Origami Gatekeepers for Solid-State Nanopores
Ruoshan Wei, Thomas G. Martin, Ulrich Rant, and Hendrik Dietz
Angewandte Chemie International Edition on-line, April 4, 2012.
DOI: 10.1002/anie.201200688

####

About Technische Universitaet Muenchen
Technische Universitaet Muenchen (TUM) is one of Europe's leading universities. It has roughly 460 professors, 9000 academic and non-academic staff, and 31,000 students. It focuses on the engineering sciences, natural sciences, life sciences, medicine, and economic sciences. After winning numerous awards, it was selected as an "Elite University" in 2006 by the Science Council (Wissenschaftsrat) and the German Research Foundation (DFG). The university's global network includes an outpost with a research campus in Singapore. TUM is dedicated to the ideal of a top-level research-based entrepreneurial university.

For more information, please click here

Contacts:
Patrick Regan

49-892-891-0515

Prof. Hendrik Dietz
Department of Physics
Technische Universität München
Am Coulombwall 4a, 85748 Garching, Germany
Tel.: +49 (0)89 289 11615

Web: http://bionano.physik.tu-muenchen.de/

Dr. Ulrich Rant
Walter Schottky Institute
Technische Universität München
Am Coulombwall 3, 85748 Garching, Germany
Tel.: +49 (0)89 289 11578

Web: http://www.wsi.tum.de/Research/AbstreitergroupE24/ResearchAreas/BioNanostructures/tabid/136/Default.aspx

Copyright © Technische Universitaet Muenchen

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Industrial Nanotech, Inc. to Publish PCAOB Audited Financials July 31st, 2014

Nanostructured metal-oxide catalyst efficiently converts CO2 to methanol: Highly reactive sites at interface of 2 nanoscale components could help overcome hurdle of using CO2 as a starting point in producing useful products July 31st, 2014

Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method July 31st, 2014

Pressure probing potential photoelectronic manufacturing compound July 31st, 2014

Nanomedicine

Arrowhead to Report Fiscal 2014 Third Quarter Financial Results- Conference Call Scheduled for Tuesday, August 12, 2014 - July 31st, 2014

New imaging agent provides better picture of the gut July 30th, 2014

Zenosense, Inc. July 29th, 2014

Optimum inertial design for self-propulsion: A new study investigates the effects of small but finite inertia on the propulsion of micro and nano-scale swimming machines July 29th, 2014

Sensors

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Compact Vibration Harvester Power Supply with Highest Efficiency Opens Door to “Fix-and-Forget” Sensor Nodes July 23rd, 2014

Nano-sized Chip "Sniffs Out" Explosives Far Better than Trained Dogs: TAU researcher's groundbreaking sensor detects miniscule concentrations of hazardous materials in the air July 23rd, 2014

Tiny laser sensor heightens bomb detection sensitivity July 19th, 2014

Discoveries

Study finds physical link to strange electronic behavior: Neutron measurements offer new clues about iron-based superconductor July 31st, 2014

Nanostructured metal-oxide catalyst efficiently converts CO2 to methanol: Highly reactive sites at interface of 2 nanoscale components could help overcome hurdle of using CO2 as a starting point in producing useful products July 31st, 2014

Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method July 31st, 2014

Pressure probing potential photoelectronic manufacturing compound July 31st, 2014

Announcements

Industrial Nanotech, Inc. to Publish PCAOB Audited Financials July 31st, 2014

Nanostructured metal-oxide catalyst efficiently converts CO2 to methanol: Highly reactive sites at interface of 2 nanoscale components could help overcome hurdle of using CO2 as a starting point in producing useful products July 31st, 2014

Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method July 31st, 2014

Pressure probing potential photoelectronic manufacturing compound July 31st, 2014

Nanobiotechnology

Harris & Harris Group Invests in Unique NYC Biotech Accelerator July 29th, 2014

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE