Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Columbia Engineering and Penn researchers increase speed of single-molecule measurements: New integrated circuit design could lead to cheaper, faster DNA sequencing

This is a photograph of the Columbia Engineering team's custom multichannel CMOS preamplifier chip, attached to a circuit board with thin gold wirebonds.

Credit: Columbia Engineering
This is a photograph of the Columbia Engineering team's custom multichannel CMOS preamplifier chip, attached to a circuit board with thin gold wirebonds.

Credit: Columbia Engineering

Abstract:
As nanotechnology becomes ever more ubiquitous, researchers are using it to make medical diagnostics smaller, faster, and cheaper, in order to better diagnose diseases, learn more about inherited traits, and more. But as sensors get smaller, measuring them becomes more difficult—there is always a tradeoff between how long any measurement takes to make and how precise it is. And when a signal is very weak, the tradeoff is especially big.

Columbia Engineering and Penn researchers increase speed of single-molecule measurements: New integrated circuit design could lead to cheaper, faster DNA sequencing

New York, NY | Posted on March 18th, 2012

A team of researchers at Columbia Engineering, led by Electrical Engineering Professor Ken Shepard, together with colleagues at the University of Pennsylvania, has figured out a way to measure nanopores—tiny holes in a thin membrane that can detect single biological molecules such as DNA and proteins—with less error than can be achieved with commercial instruments. They have miniaturized the measurement by designing a custom integrated circuit using commercial semiconductor technology, building the nanopore measurement around the new amplifier chip. Their research will be published in the Advance Online Publication on Nature Methods's website on 18 March at 1400 (2pm) US Eastern time/ 1800 London time.

Nanopores are exciting scientists because they may lead to extremely low-cost and fast DNA sequencing. But the signals from nanopores are very weak, so it is critically important to measure them as cleanly as possible.

"We put a tiny amplifier chip directly into the liquid chamber next to the nanopore, and the signals are so clean that we can see single molecules passing through the pore in only one microsecond," says Jacob Rosenstein, a Ph.D. candidate in electrical engineering at Columbia Engineering and lead author of the paper. "Previously, scientists could only see molecules that stay in the pore for more than 10 microseconds."

Many single-molecule measurements are currently made using optical techniques, which use fluorescent molecules that emit photons at a particular wavelength. But, while fluorescence is very powerful, its major limitation is that each molecule usually produces only a few thousand photons per second. "This means you can't see anything that happens faster than a few milliseconds, because any image you could take would be too dim," explains Shepard, who is Rosenstein's advisor. "On the other hand, if you can use techniques that measure electrons or ions, you can get billions of signals per second. The problem is that for electronic measurements there is no equivalent to a fluorescent wavelength filter, so even though the signal comes through, it is often buried in background noise."

Shepard's group has been interested in single-molecule measurements for several years looking at a variety of novel transduction platforms. They began working with nanopore sensors after Marija Drndic, a professor of physics at the University of Pennsylvania, gave a seminar at Columbia Engineering in 2009. "We saw that nearly everybody else measures nanopores using classical electrophysiology amplifiers, which are mostly optimized for slower measurements," notes Shepard. "So we designed our own integrated circuit instead."

Rosenstein designed the new electronics and did much of the lab work. Drndic's group at the University of Pennsylvania fabricated the nanopores that the team then measured in their new system.

"While most groups are trying to slow down DNA, our approach is to build faster electronics," says Drndic. "We combined the most sensitive electronics with the most sensitive solid-state nanopores."

"It's very exciting to be able to make purely electronic measurements of single molecules," says Rosenstein. "The setup for nanopore measurements is very simple and portable. It doesn't require a complicated microscope or high powered instruments; it just requires attention to detail. You can easily imagine nanopore technology having a major impact on DNA sequencing and other medical applications within the next few years."

Shepard's group is continuing to improve these techniques. "With a next-generation design," he says, "we may be able to get a further 10X improvement, and measure things that last only 100 nanoseconds. Our lab is also working with other electronic single-molecule techniques based on carbon nanotube transistors, which can leverage similar electronic circuits. This is an exciting time!"

This research has been funded by the National Institutes of Health, the Semiconductor Research Corporation, and the Office of Naval Research.

####

About Columbia University
Columbia University's Fu Foundation School of Engineering and Applied Science, founded in 1864, offers programs in nine departments to both undergraduate and graduate students. With facilities specifically designed and equipped to meet the laboratory and research needs of faculty and students, Columbia Engineering is home to NSF-NIH funded centers in genomic science, molecular nanostructures, materials science, and energy, as well as one of the world's leading programs in financial engineering. These interdisciplinary centers are leading the way in their respective fields while individual groups of engineers and scientists collaborate to solve some of modern society's more difficult challenges.

For more information, please click here

Contacts:
Holly Evarts

347-453-7408

Copyright © Columbia University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

JPK announces expansion of its global sales and service activities in China and USA April 15th, 2014

Nanobiotix Appoints Thierry Otin as Head of Manufacturing and Supply April 15th, 2014

PAM-XIAMEN Offers UV LED wafer April 15th, 2014

Engineers develop new materials for hydrogen storage April 15th, 2014

Govt.-Legislation/Regulation/Funding/Policy

Engineers develop new materials for hydrogen storage April 15th, 2014

Tiny particles could help verify goods: Chemical engineers hope smartphone-readable microparticles could crack down on counterfeiting April 15th, 2014

A molecular approach to solar power: Switchable material could harness the power of the sun — even when it’s not shining April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Chip Technology

Scientists open door to better solar cells, superconductors and hard-drives: Research enhances understanding of materials interfaces April 14th, 2014

Obducat has launched a new generation of SINDRE® Nano Imprint production system April 11th, 2014

Scientists in Singapore develop novel ultra-fast electrical circuits using light-generated tunneling currents April 10th, 2014

Clean Shot at Manufacturing Course…For Less April 9th, 2014

Nanomedicine

Nanobiotix Appoints Thierry Otin as Head of Manufacturing and Supply April 15th, 2014

PAM-XIAMEN Offers UV LED wafer April 15th, 2014

Nanocrystalline cellulose modified into an efficient viral inhibitor April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Discoveries

Engineers develop new materials for hydrogen storage April 15th, 2014

Nanocrystalline cellulose modified into an efficient viral inhibitor April 15th, 2014

Tiny particles could help verify goods: Chemical engineers hope smartphone-readable microparticles could crack down on counterfeiting April 15th, 2014

A molecular approach to solar power: Switchable material could harness the power of the sun — even when it’s not shining April 15th, 2014

Announcements

Tiny particles could help verify goods: Chemical engineers hope smartphone-readable microparticles could crack down on counterfeiting April 15th, 2014

A molecular approach to solar power: Switchable material could harness the power of the sun — even when it’s not shining April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone April 15th, 2014

Military

Tiny particles could help verify goods: Chemical engineers hope smartphone-readable microparticles could crack down on counterfeiting April 15th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Scalable CVD process for making 2-D molybdenum diselenide: Rice, NTU scientists unveil CVD production for coveted 2-D semiconductor April 8th, 2014

Rebar technique strengthens case for graphene: Rice University lab makes hybrid nanotube-graphene material that promises to simplify manufacturing April 7th, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE