Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Targeted immune stimulation based on DNA Nanotechnology

Abstract:
DNA is usually known as the genetic code for protein synthesis in all living organisms. The application of DNA as a molecular building block on the other hand, allows for the construction of sophisticated nanoscopic shapes that are built entirely from DNA. In particular the recent invention of the so-called DNA origami method facilitates the fabrication of almost all imaginable 3D shapes. Here a phage-based DNA strand is used as a scaffold that is woven into shape by hundreds of short staple oligonucleotides. The outstanding advantage of DNA-based self-assembly is that during a single fabrication process billion exact copies of the designed DNA nanostructure are produced in parallel.

Targeted immune stimulation based on DNA Nanotechnology

Munich, Germany | Posted on December 22nd, 2011

Now Prof. Tim Liedl, a member of NIM, and his team developed a DNA origami construct that serves as a carrier system to selectively stimulate immune responses of living cells. Together with the group of Prof. Carole Bourquin from the Klinikum der Universität München (KUM) the biophysicists investigated the systematic immune stimulatory effect and the potential cytotoxicity of these DNA nanostructures.

Our innate immune system can detect invasive organisms via a specific DNA motif, the so called CpG sequences ("Cytosine - phosphate - Guanine") which are prevalent in viruses and bacteria. When these sequences are internalized by certain immune cells, they are recognized by endosomal receptors like the Toll-Like Receptor 9 (TLR-9) which subsequently activate the immune system. The Toll-Like Receptors became famous at the latest in 2011, when Bruce Beutler and Jules Hoffmann received the Nobel Prize for their research on these kinds of receptors.

Verena Schüller from the Liedl group and her colleagues decorated a DNA origami construct with artificial CpG sequences and used it as an efficient non-toxic carrier system into cells. Together with the team of Carole Bourquin they demonstrated a selective immune stimulating effect of the DNA complexes by measuring the interleukin secretion of the cells as an indicator for immune activation. Such artificial nanostructures could act in future applications as target-selective delivery vehicles for the development of novel and non-toxic vaccine adjuvants or carrier systems in tumor immunotherapy.

Publication:

Cellular Immunostimulation by CpG-Sequence-Coated DNA Origami Structures. Verena Schüller, Simon Heidegger, Nadja Sandholzer, Philipp Nickels, Nina Suharta, Stefan Endres, Carole Bourquin and Tim Liedl. ACS Nano, 2011

####

For more information, please click here

Copyright © Nanosystems Initiative Munich

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Looking for the quantum frontier: Beyond classical computing without fault-tolerance? April 27th, 2017

Metal nanoparticles induced visible-light photocatalysis: Mechanisms, applications, ways of promoting catalytic activity and outlook April 27th, 2017

Arrowhead Pharmaceuticals to Webcast Fiscal 2017 Second Quarter Results April 27th, 2017

California Research Alliance by BASF establishes more than 25 research projects in three years April 26th, 2017

Nanomedicine

Arrowhead Pharmaceuticals to Webcast Fiscal 2017 Second Quarter Results April 27th, 2017

New Product Nanoparticle preparation from Intertronics with new Thinky NP-100 Nano Pulveriser April 26th, 2017

Nanoparticle vaccine shows potential as immunotherapy to fight multiple cancer types April 24th, 2017

Arrowhead Presents ARC-520 and ARC-521 Clinical Data at The International Liver Congress(TM) April 20th, 2017

Discoveries

Looking for the quantum frontier: Beyond classical computing without fault-tolerance? April 27th, 2017

Metal nanoparticles induced visible-light photocatalysis: Mechanisms, applications, ways of promoting catalytic activity and outlook April 27th, 2017

Geoffrey Beach: Drawn to explore magnetism: Materials researcher is working on the magnetic memory of the future April 25th, 2017

Using light to propel water : With new method, MIT engineers can control and separate fluids on a surface using only visible light April 25th, 2017

Announcements

Looking for the quantum frontier: Beyond classical computing without fault-tolerance? April 27th, 2017

Metal nanoparticles induced visible-light photocatalysis: Mechanisms, applications, ways of promoting catalytic activity and outlook April 27th, 2017

Arrowhead Pharmaceuticals to Webcast Fiscal 2017 Second Quarter Results April 27th, 2017

Video captures bubble-blowing battery in action: Researchers propose how bubbles form, could lead to smaller lithium-air batteries April 26th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project