Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > 'Label-free' imaging tool tracks nanotubes in cells, blood for biomedical research

Researchers have demonstrated a new imaging tool for tracking structures called single-wall carbon nanotubes in living cells and the bloodstream, work that could aid efforts to perfect their use in laboratory or medical applications. Here, the imaging system detects both metallic and semiconducting nanotubes, false-colored in red and green, in live hamster cells. (Weldon School of Biomedical Engineering, Purdue University)
Researchers have demonstrated a new imaging tool for tracking structures called single-wall carbon nanotubes in living cells and the bloodstream, work that could aid efforts to perfect their use in laboratory or medical applications. Here, the imaging system detects both metallic and semiconducting nanotubes, false-colored in red and green, in live hamster cells.

(Weldon School of Biomedical Engineering, Purdue University)

Abstract:
Label-Free Imaging of Semiconducting and Metallic Carbon Nanotubes in Cells and Mice Using Transient Absorption Microscopy

Ling Tong1, Yuxiang Liu2, Bridget D. Dolash3, Yookyung Jung4, Mikhail N. Slipchenko2, Donald E. Bergstrom3,5 and Ji-Xin Cheng1,2,5*

1Department of Chemistry; 2Weldon School of Biomedical Engineering, 3Department of Medical Chemistry and Molecular Pharmacology; 4Department of Physics,5 Birck Nanotechnology Center, Purdue University

As interest in the potential biomedical applications of carbon nanotubes increases, there is a need for methods that can image nanotubes in live cells, tissues and animals. Although techniques such as Raman, photoacoustic and near-infrared photoluminescence imaging have been used to visualize nanotubes in biological environments, these techniques are limited because nanotubes provide only weak photoluminescence and low Raman scattering and it remains difficult to image both semiconducting and metallic nanotubes at the same time. Here, we show that transient absorption microscopy offers a label-free method to image both semiconducting and metallic single-walled carbon nanotubes in vitro and in vivo, in real time, with submicrometre resolution. By using appropriate near-infrared excitation wavelengths, we detect strong transient absorption signals with opposite phases from semiconducting and metallic nanotubes. Our method separates background signals generated by red blood cells and this allows us to follow the movement of both types of nanotubes inside cells and in the blood circulation and organs of mice without any significant damaging effects.

'Label-free' imaging tool tracks nanotubes in cells, blood for biomedical research

West Lafayette, IN | Posted on December 5th, 2011

Researchers have demonstrated a new imaging tool for tracking structures called carbon nanotubes in living cells and the bloodstream, which could aid efforts to perfect their use in biomedical research and clinical medicine.

The structures have potential applications in drug delivery to treat diseases and imaging for cancer research. Two types of nanotubes are created in the manufacturing process, metallic and semiconducting. Until now, however, there has been no technique to see both types in living cells and the bloodstream, said Ji-Xin Cheng, an associate professor of biomedical engineering and chemistry at Purdue University.

The imaging technique, called transient absorption, uses a pulsing near-infrared laser to deposit energy into the nanotubes, which then are probed by a second near-infrared laser.

The researchers have overcome key obstacles in using the imaging technology, detecting and monitoring the nanotubes in live cells and laboratory mice, Cheng said.

"Because we can do this at high speed, we can see what's happening in real time as the nanotubes are circulating in the bloodstream," he said.

Findings are detailed in a research paper posted online Sunday (Dec. 4) in the journal Nature Nanotechnology.

The imaging technique is "label free," meaning it does not require that the nanotubes be marked with dyes, making it potentially practical for research and medicine, Cheng said.

"It's a fundamental tool for research that will provide information for the scientific community to learn how to perfect the use of nanotubes for biomedical and clinical applications," he said.

The conventional imaging method uses luminescence, which is limited because it detects the semiconducting nanotubes but not the metallic ones.

The nanotubes have a diameter of about 1 nanometer, or roughly the length of 10 hydrogen atoms strung together, making them far too small to be seen with a conventional light microscope. One challenge in using the transient absorption imaging system for living cells was to eliminate the interference caused by the background glow of red blood cells, which is brighter than the nanotubes.

The researchers solved this problem by separating the signals from red blood cells and nanotubes in two separate "channels." Light from the red blood cells is slightly delayed compared to light emitted by the nanotubes. The two types of signals are "phase separated" by restricting them to different channels based on this delay.

Researchers used the technique to see nanotubes circulating in the blood vessels of mice earlobes.

"This is important for drug delivery because you want to know how long nanotubes remain in blood vessels after they are injected," Cheng said. "So you need to visualize them in real time circulating in the bloodstream."

The structures, called single-wall carbon nanotubes, are formed by rolling up a one-atom-thick layer of graphite called graphene. The nanotubes are inherently hydrophobic, so some of the nanotubes used in the study were coated with DNA to make them water-soluble, which is required for them to be transported in the bloodstream and into cells.

The researchers also have taken images of nanotubes in the liver and other organs to study their distribution in mice, and they are using the imaging technique to study other nanomaterials such as graphene.

The paper was written by doctoral student Ling Tong; postdoctoral research associate Yuxiang Liu; doctoral students Bridget D. Dolash and Yookyung Jung; biomedical engineering research scientist Mikhail N. Slipchenko; Donald E. Bergstrom, the Walther Professor of Medicinal Chemistry; and Cheng.

The research is funded by the National Science Foundation.

####

For more information, please click here

Contacts:
Writer:
Emil Venere
765-494-4709


Source:
Ji-Xin Cheng
765-494-4335

Copyright © Purdue University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Purdue Weldon School of Biomedical Engineering

Purdue Department of Chemistry

Chen Yang

Related News Press

News and information

Hybrid nanoantennas -- next-generation platform for ultradense data recording April 28th, 2016

Superfast light source made from artificial atom April 28th, 2016

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

JPK reports on the use of a NanoWizard AFM system at the University of Kaiserslautern to study the interaction of bacteria with microstructured surfaces April 28th, 2016

Imaging

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

JPK reports on the use of a NanoWizard AFM system at the University of Kaiserslautern to study the interaction of bacteria with microstructured surfaces April 28th, 2016

Bruker Introduces First of Its Kind Dimensional Analysis System: The Novel Contour CMM™ System Fully Integrates 3D Coordinate Measurements with Nanoscale Surface Height, Texture, Waviness and Form Characterization April 26th, 2016

Bruker Introduces Dimension FastScan Pro Industrial AFM: Providing Nanometer-Resolution at High Scan Rates for up to 300-mm Samples April 26th, 2016

Graphene/ Graphite

University of Illinois researchers create 1-step graphene patterning method April 27th, 2016

Atomic magnets using hydrogen and graphene April 27th, 2016

Govt.-Legislation/Regulation/Funding/Policy

Nanoparticles hold promise as double-edged sword against genital herpes April 28th, 2016

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

NREL theory establishes a path to high-performance 2-D semiconductor devices April 27th, 2016

Researchers create artificial protein to control assembly of buckyballs April 27th, 2016

Chip Technology

Researchers create a first frequency comb of time-bin entangled qubits: Discovery is a significant step toward multi-channel quantum communication and higher capacity quantum computers April 28th, 2016

University of Illinois researchers create 1-step graphene patterning method April 27th, 2016

NREL theory establishes a path to high-performance 2-D semiconductor devices April 27th, 2016

Atomic magnets using hydrogen and graphene April 27th, 2016

Nanotubes/Buckyballs/Fullerenes

NREL finds nanotube semiconductors well-suited for PV systems April 27th, 2016

Researchers create artificial protein to control assembly of buckyballs April 27th, 2016

Cleaning up hybrid battery electrodes improves capacity and lifespan: New way of building supercapacitor-battery electrodes eliminates interference from inactive components April 22nd, 2016

Nature Photonics: Light source for quicker computer chips: Waveguide with integrated carbon nanotubes for conversion of electric signals into light / quicker computer chips are feasible / publication in Nature Photonics April 21st, 2016

Nanomedicine

Nanoparticles hold promise as double-edged sword against genital herpes April 28th, 2016

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

JPK reports on the use of a NanoWizard AFM system at the University of Kaiserslautern to study the interaction of bacteria with microstructured surfaces April 28th, 2016

Discoveries

Nanoparticles hold promise as double-edged sword against genital herpes April 28th, 2016

Researchers create a first frequency comb of time-bin entangled qubits: Discovery is a significant step toward multi-channel quantum communication and higher capacity quantum computers April 28th, 2016

Superfast light source made from artificial atom April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

Announcements

Hybrid nanoantennas -- next-generation platform for ultradense data recording April 28th, 2016

Superfast light source made from artificial atom April 28th, 2016

Arrowhead Pharmaceuticals Files for Regulatory Clearance to Begin Phase 1/2 Study of ARC-521 April 28th, 2016

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes April 28th, 2016

Tools

JPK reports on the use of a NanoWizard AFM system at the University of Kaiserslautern to study the interaction of bacteria with microstructured surfaces April 28th, 2016

Chemists use DNA to build the world's tiniest thermometer April 27th, 2016

Bruker Introduces First of Its Kind Dimensional Analysis System: The Novel Contour CMM™ System Fully Integrates 3D Coordinate Measurements with Nanoscale Surface Height, Texture, Waviness and Form Characterization April 26th, 2016

Bruker Introduces Dimension FastScan Pro Industrial AFM: Providing Nanometer-Resolution at High Scan Rates for up to 300-mm Samples April 26th, 2016

Safety-Nanoparticles/Risk management

Scientists propose non-animal tools for assessing the toxicity of nanomaterials: Particle and Fibre Toxicology publishes recommendations from expert group meeting April 26th, 2016

The impact of anti-odor clothing on the environment March 31st, 2016

SUNY Poly, in Collaboration with the George Washington School of Medicine and Health Sciences and Stony Brook University, Demonstrates Pioneering Method to Visualize and Identify Engineered Nanoparticles in Tissue March 25th, 2016

Microagents with revolutionary potential March 24th, 2016

Photonics/Optics/Lasers

Researchers create a first frequency comb of time-bin entangled qubits: Discovery is a significant step toward multi-channel quantum communication and higher capacity quantum computers April 28th, 2016

Hybrid nanoantennas -- next-generation platform for ultradense data recording April 28th, 2016

NREL theory establishes a path to high-performance 2-D semiconductor devices April 27th, 2016

Physicists detect the enigmatic spin momentum of light April 26th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic