Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > Nanoparticles Harvest Invisible Cancer Biomarkers

Abstract:
Cancer biologists have long presumed that tumor cells shed telltale markers into the blood and that finding these blood-borne biomarkers could provide an early indicator that cancer is developing somewhere in the body. While there has been some progress in finding such markers, researchers have been largely stymied in this pursuit by the fact that such proteins are present in trace amounts that are cloaked by the few proteins present in far larger amounts, such as albumin and antibodies.

Nanoparticles Harvest Invisible Cancer Biomarkers

Bethesda, MD | Posted on November 17th, 2011

Now, a research team at the George Mason University has shown that they can fish out the "invisible" proteins masked by albumin and other high concentration proteins using porous nanoparticles decorated with a series of chemical baits, each designed to harvest specific types of trace proteins from body fluids. Better yet, hooking these proteins onto the baits, which are buried within the pores of the nanoparticles, protects them from degradation until they can be released and analyzed using mass spectroscopy.

Alessandra Luchini led the international team of investigators that designed and tested the bait-laden core-shell nanoparticles. The investigators published their work in the Journal of the American Chemical Society.

Core-shell hydrogel nanoparticles have been touted as potential protein drug delivery vehicles that would sequester these drugs from the action of protein degrading enzymes in blood until they reach their targets in the body. Luchini and her collaborators turned this paradigm on its head, choosing to use them to instead remove proteins from the blood until they can be safely collected. The key was identifying a set of 17 molecules that the researchers could attach inside the cavity structures that exist in hydrogels. These cavities are large enough to let most proteins in, but are too small for the relatively gigantic proteins that are overwhelmingly prevalent in blood and other biological fluids. To prevent smaller fragments of albumin, which are also a major blood component, from entering the nanoparticles, the investigators added to the outer shell the chemical vinylsulfonic acid, or VSA, that actively excludes albumin fragments of all sizes.

For bait molecules, Luchini and her colleagues started with a few dye molecules that biochemists have used as protein binding agents and inhibitors of protein-protein interactions in chromatography experiments. Working from the chemical structures of these molecules, the investigators created a set of dyes that they could then react with their core-shell hydrogel nanoparticles. They then mix the resulting nanoparticles with a biological fluid - whole blood, urine, and sweat, for example - and incubated for 15 minutes. The particles are collected using a centrifuge, and the captured proteins are washed out for analysis using a set of buffers.

Luchini's team showed that the nanoparticles enabled a 10,000-fold effective amplification of protein levels in the wash fluid compared to their concentration in blood. As a result, they were able to use mass spectrometry to identify a variety of proteins that were previously undetectable in blood using any type of method that would be clinically useful.

####

About The National Cancer Institute (NCI)
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Contacts:
National Cancer Institute
Office of Technology & Industrial Relations
ATTN: NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A49
31 Center Drive , MSC 2580
Bethesda , MD 20892-2580

Copyright © The National Cancer Institute (NCI)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

View abstract - "Multifunctional Core-Shell Nanoparticles: Discovery of Previously Invisible Biomarkers."

Related News Press

News and information

Researchers develop artificial building blocks of life March 8th, 2024

How surface roughness influences the adhesion of soft materials: Research team discovers universal mechanism that leads to adhesion hysteresis in soft materials March 8th, 2024

Two-dimensional bimetallic selenium-containing metal-organic frameworks and their calcinated derivatives as electrocatalysts for overall water splitting March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

Govt.-Legislation/Regulation/Funding/Policy

What heat can tell us about battery chemistry: using the Peltier effect to study lithium-ion cells March 8th, 2024

Researchers’ approach may protect quantum computers from attacks March 8th, 2024

The Access to Advanced Health Institute receives up to $12.7 million to develop novel nanoalum adjuvant formulation for better protection against tuberculosis and pandemic influenza March 8th, 2024

Optically trapped quantum droplets of light can bind together to form macroscopic complexes March 8th, 2024

Nanomedicine

High-tech 'paint' could spare patients repeated surgeries March 8th, 2024

Researchers develop artificial building blocks of life March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

The Access to Advanced Health Institute receives up to $12.7 million to develop novel nanoalum adjuvant formulation for better protection against tuberculosis and pandemic influenza March 8th, 2024

Discoveries

What heat can tell us about battery chemistry: using the Peltier effect to study lithium-ion cells March 8th, 2024

Researchers’ approach may protect quantum computers from attacks March 8th, 2024

High-tech 'paint' could spare patients repeated surgeries March 8th, 2024

Nanoscale CL thermometry with lanthanide-doped heavy-metal oxide in TEM March 8th, 2024

Announcements

What heat can tell us about battery chemistry: using the Peltier effect to study lithium-ion cells March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

The Access to Advanced Health Institute receives up to $12.7 million to develop novel nanoalum adjuvant formulation for better protection against tuberculosis and pandemic influenza March 8th, 2024

Nanoscale CL thermometry with lanthanide-doped heavy-metal oxide in TEM March 8th, 2024

Research partnerships

Researchers’ approach may protect quantum computers from attacks March 8th, 2024

How surface roughness influences the adhesion of soft materials: Research team discovers universal mechanism that leads to adhesion hysteresis in soft materials March 8th, 2024

'Sudden death' of quantum fluctuations defies current theories of superconductivity: Study challenges the conventional wisdom of superconducting quantum transitions January 12th, 2024

Development of zinc oxide nanopagoda array photoelectrode: photoelectrochemical water-splitting hydrogen production January 12th, 2024

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project