Nanotechnology Now





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Nanoparticles Harvest Invisible Cancer Biomarkers

Abstract:
Cancer biologists have long presumed that tumor cells shed telltale markers into the blood and that finding these blood-borne biomarkers could provide an early indicator that cancer is developing somewhere in the body. While there has been some progress in finding such markers, researchers have been largely stymied in this pursuit by the fact that such proteins are present in trace amounts that are cloaked by the few proteins present in far larger amounts, such as albumin and antibodies.

Nanoparticles Harvest Invisible Cancer Biomarkers

Bethesda, MD | Posted on November 17th, 2011

Now, a research team at the George Mason University has shown that they can fish out the "invisible" proteins masked by albumin and other high concentration proteins using porous nanoparticles decorated with a series of chemical baits, each designed to harvest specific types of trace proteins from body fluids. Better yet, hooking these proteins onto the baits, which are buried within the pores of the nanoparticles, protects them from degradation until they can be released and analyzed using mass spectroscopy.

Alessandra Luchini led the international team of investigators that designed and tested the bait-laden core-shell nanoparticles. The investigators published their work in the Journal of the American Chemical Society.

Core-shell hydrogel nanoparticles have been touted as potential protein drug delivery vehicles that would sequester these drugs from the action of protein degrading enzymes in blood until they reach their targets in the body. Luchini and her collaborators turned this paradigm on its head, choosing to use them to instead remove proteins from the blood until they can be safely collected. The key was identifying a set of 17 molecules that the researchers could attach inside the cavity structures that exist in hydrogels. These cavities are large enough to let most proteins in, but are too small for the relatively gigantic proteins that are overwhelmingly prevalent in blood and other biological fluids. To prevent smaller fragments of albumin, which are also a major blood component, from entering the nanoparticles, the investigators added to the outer shell the chemical vinylsulfonic acid, or VSA, that actively excludes albumin fragments of all sizes.

For bait molecules, Luchini and her colleagues started with a few dye molecules that biochemists have used as protein binding agents and inhibitors of protein-protein interactions in chromatography experiments. Working from the chemical structures of these molecules, the investigators created a set of dyes that they could then react with their core-shell hydrogel nanoparticles. They then mix the resulting nanoparticles with a biological fluid - whole blood, urine, and sweat, for example - and incubated for 15 minutes. The particles are collected using a centrifuge, and the captured proteins are washed out for analysis using a set of buffers.

Luchini's team showed that the nanoparticles enabled a 10,000-fold effective amplification of protein levels in the wash fluid compared to their concentration in blood. As a result, they were able to use mass spectrometry to identify a variety of proteins that were previously undetectable in blood using any type of method that would be clinically useful.

####

About The National Cancer Institute (NCI)
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Contacts:
National Cancer Institute
Office of Technology & Industrial Relations
ATTN: NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A49
31 Center Drive , MSC 2580
Bethesda , MD 20892-2580

Copyright © The National Cancer Institute (NCI)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

View abstract - "Multifunctional Core-Shell Nanoparticles: Discovery of Previously Invisible Biomarkers."

Related News Press

News and information

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

Two UCSB Professors Receive Early Career Research Awards: The Department of Energy’s award for young scientists acknowledges UC Santa Barbara’s standing as a top tier research institution May 29th, 2015

Global Carbon Nanotubes (CNT) Market Expected To Reach USD 3.42 Billion By 2022 May 29th, 2015

New chip makes testing for antibiotic-resistant bacteria faster, easier: Researchers at the University of Toronto design diagnostic chip to reduce testing time from days to one hour, allowing doctors to pick the right antibiotic the first time May 28th, 2015

Govt.-Legislation/Regulation/Funding/Policy

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

Physicists precisely measure interaction between atoms and carbon surfaces May 28th, 2015

Linking superconductivity and structure May 28th, 2015

Chemists discover key reaction mechanism behind the highly touted sodium-oxygen battery May 28th, 2015

Nanomedicine

New chip makes testing for antibiotic-resistant bacteria faster, easier: Researchers at the University of Toronto design diagnostic chip to reduce testing time from days to one hour, allowing doctors to pick the right antibiotic the first time May 28th, 2015

Arrowhead to Present at Jefferies 2015 Healthcare Conference May 27th, 2015

Seeing the action: UCSB researchers develop a novel device to image the minute forces and actions involved in cell membrane hemifusion May 27th, 2015

Nanotechnology identifies brain tumor types through MRI 'virtual biopsy' in animal studies: If results are confirmed in humans, tumor cells could someday be diagnosed by MRI imaging and treated with tumor-specific IV injections; new NIH grant will fund future study May 27th, 2015

Discoveries

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

Nano-capsules designed for diagnosing malignant tumours: Japanese researchers have developed adaptable nano-capsules that can help in the diagnosis of glioblastoma cells - a highly invasive form of brain tumours May 28th, 2015

Physicists precisely measure interaction between atoms and carbon surfaces May 28th, 2015

Linking superconductivity and structure May 28th, 2015

Announcements

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

Two UCSB Professors Receive Early Career Research Awards: The Department of Energy’s award for young scientists acknowledges UC Santa Barbara’s standing as a top tier research institution May 29th, 2015

Global Carbon Nanotubes (CNT) Market Expected To Reach USD 3.42 Billion By 2022 May 29th, 2015

New chip makes testing for antibiotic-resistant bacteria faster, easier: Researchers at the University of Toronto design diagnostic chip to reduce testing time from days to one hour, allowing doctors to pick the right antibiotic the first time May 28th, 2015

Research partnerships

Linking superconductivity and structure May 28th, 2015

How spacetime is built by quantum entanglement: New insight into unification of general relativity and quantum mechanics May 28th, 2015

Collaboration could lead to biodegradable computer chips May 28th, 2015

Supercomputer unlocks secrets of plant cells to pave the way for more resilient crops: IBM partners with University of Melbourne and UQ May 21st, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project