Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > New biosensor benefits from melding of carbon nanotubes, DNA

Abstract:
Microbiosensors Based on DNA Modified Single-Walled Carbon Nanotube and Pt Black Nanocomposites

Jin Shi, Tae-Gon Cha, Jonathan C. Claussen, Alfred R. Diggs, Jong Hyun Choi and D. Marshall Porterfield

Glucose and ATP biosensors have important applications in diagnostics and research. Biosensors based on conventional materials suffer from low sensitivity and low spatial resolution. Our previous work has shown that combining single-walled carbon nanotubes (SWCNTs) with Pt nanoparticles can significantly enhance the performance of electrochemical biosensors. The immobilization of SWCNTs on biosensors remains challenging due to the aqueous insolubility originating from van der Waals forces. In this study, we used single-stranded DNA (ssDNA) to modify SWCNTs to increase solubility in water. This allowed us to explore new schemes of combining ssDNA-SWCNT and Pt black in aqueous media systems. The result is a nanocomposite with enhanced biosensor performance. The surface morphology, electroactive surface area, and electrocatalytic performance of different fabrication protocols were studied and compared. The ssDNA-SWCNT/Pt black nanocomposite constructed by a layered scheme proved most effective in terms of biosensor activity. The key feature of this protocol is the exploitation of ssDNA-SWCNTs as molecular templates for Pt black electrodeposition. The glucose and ATP microbiosensors fabricated on this platform exhibited high sensitivity (817.3 nA/mM and 45.6 nA/mM, respectively), wide linear range (up to 7 mM and 510 μM), low limit of detection (1 μM and 2 μM) and desirable selectivity. This work is significant to biosensor development because this is the first demonstration of ssDNA-SWCNT/Pt black nanocomposite as a platform for constructing both single-enzyme and multi-enzyme biosensors for physiological applications.

New biosensor benefits from melding of carbon nanotubes, DNA

West Lafayette, IN | Posted on November 14th, 2011

Purdue University scientists have developed a method for stacking synthetic DNA and carbon nanotubes onto a biosensor electrode, a development that may lead to more accurate measurements for research related to diabetes and other diseases.

Standard sensors employ metal electrodes coated with enzymes that react with compounds and produce an electrical signal that can be measured. But the inefficiency of those sensors leads to imperfect measurements.

Carbon nanotubes, cylindrically shaped carbon molecules known to have excellent thermal and electrical properties, have been seen as a possibility for improving sensor performance. The problem is that the materials are not fully compatible with water, which limits their application in biological fluids.

Marshall Porterfield, a professor of agricultural and biological engineering and biomedical engineering, and Jong Hyun Choi, an assistant professor of mechanical engineering, have found a solution. Their findings, reported in the journal The Analyst, describe a sensor that essentially builds itself.

"In the future, we will be able to create a DNA sequence that is complementary to the carbon nanotubes and is compatible with specific biosensor enzymes for the many different compounds we want to measure," Porterfield said. "It will be a self-assembling platform for biosensors at the biomolecular level."

Choi developed a synthetic DNA that will attach to the surface of the carbon nanotubes and make them more water-soluble.

"Once the carbon nanotubes are in a solution, you only have to place the electrode into the solution and charge it. The carbon nanotubes will then coat the surface," Choi said.

The electrode coated with carbon nanotubes will attract the enzymes to finish the sensor's assembly.

The sensor described in the findings was designed for glucose. But Porterfield said it could be easily adapted for various compounds.

"You could mass produce these sensors for diabetes, for example, for insulin management for diabetic patients," Porterfield said.

Porterfield said it may one day be possible to develop other sensors using this technology that could lead to more personalized medicines that could test in real time the effectiveness of drugs on their targets as with cancer patients.

Porterfield said he would continue to develop biosensors to detect different compounds.

The National Institutes of Health and the Office of Naval Research funded the research.

####

For more information, please click here

Contacts:
Writer:
Brian Wallheimer
765-496-2050


Sources:
Marshall Porterfield
765-494-1190


Jong Hyun Choi
765-496-3562


Ag Communications:
(765) 494-2722
Keith Robinson

Copyright © Purdue University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Dartmouth team creates new method to control quantum systems May 24th, 2016

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Attosecond physics: A switch for light-wave electronics May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Govt.-Legislation/Regulation/Funding/Policy

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Nanoscale Trojan horses treat inflammation May 24th, 2016

Programmable materials find strength in molecular repetition May 23rd, 2016

Nanotubes/Buckyballs/Fullerenes

Programmable materials find strength in molecular repetition May 23rd, 2016

Nanotubes are beacons in cancer-imaging technique: Rice University researchers use spectral triangulation to pinpoint location of tumors May 21st, 2016

Unveiling the electron's motion in a carbon nanocoil: Development of a precise resistivity measurement system for quasi-one-dimensional nanomaterials using a focused ion beam May 16th, 2016

New research shows how silver could be the key to gold-standard flexible gadgets: Silver nanowires are an ideal material for current and future flexible touch-screen technologies May 13th, 2016

Sensors

Dartmouth team creates new method to control quantum systems May 24th, 2016

Electronic device detects molecules linked to cancer, Alzheimer's and Parkinson's: An inexpensive portable biosensor has been developed by researchers at Brazil's National Nanotechnology Laboratory with FAPESP's support May 20th, 2016

Making organs transparent to improve nanomedicine (video) May 13th, 2016

Scientists take a major leap toward a 'perfect' quantum metamaterial: Berkeley Lab, UC Berkeley researchers lead study that uses trapped atoms in an artificial crystal of light May 13th, 2016

Discoveries

Dartmouth team creates new method to control quantum systems May 24th, 2016

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Attosecond physics: A switch for light-wave electronics May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Announcements

Dartmouth team creates new method to control quantum systems May 24th, 2016

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Attosecond physics: A switch for light-wave electronics May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Military

Nanoscale Trojan horses treat inflammation May 24th, 2016

Programmable materials find strength in molecular repetition May 23rd, 2016

Rice de-icer gains anti-icing properties: Dual-function, graphene-based material good for aircraft, extreme environments May 23rd, 2016

UW researchers unleash graphene 'tiger' for more efficient optoelectronics May 16th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic