Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button

Home > Press > Into the (Mis)fold: a Diagnostic Tool for Proteins Berkeley Lab developed technique could help pinpoint Alzheimer’s in its early stages

Abstract:
Alzheimer's disease is the most common form of dementia, currently affecting more than 35 million people worldwide. Although many genetic and hereditary factors are thought to contribute to the telltale deterioration of memory and cognitive functions resulting from Alzheimer's, a central aspect to this disease is an accumulation of misfolded proteins in the brain.

Into the (Mis)fold: a Diagnostic Tool for Proteins Berkeley Lab developed technique could help pinpoint Alzheimer’s in its early stages

Berkeley, CA | Posted on June 1st, 2011

Now, scientists at Berkeley Lab have engineered a universal, highly sensitive technique for detecting misfolded proteins in biological fluids. This groundbreaking nanoscience capability could help pinpoint Alzheimer's in its early stages and enable researchers to discover new therapies for this devastating disease.

When a protein doesn't fold into its normal shape, it also doesn't perform its normal functions. This disruption in behavior could lead to proteins that aggregate into plaques or deposits and become toxic to cells. In Alzheimer's disease, aggregates of a protein called beta-amyloid form in the central nervous system, causing damage to cells in the brain and triggering dementia.

An analytical capability for measuring tiny clusters of these proteins—before irreversible damage occurs—would be a powerful tool in the early detection of Alzheimer's and other misfolded protein diseases. However, despite significant research efforts, there are currently no diagnostic tools available to selectively detect small-scale aggregates of misfolded proteins in biological fluids, such as blood or spinal fluid.

"This collaboration illustrates how a biomedical problem can also be a nanoscience problem, in which a chemical reagent is needed to recognize partially aggregated proteins," said Ron Zuckermann, Director of the Biological Nanostructures Facility at the Molecular Foundry, a nanoscience user facility at Berkeley Lab. "We were faced with the challenge of synthesizing a material that's capable of specifically detecting this aggregated protein and not any of the other proteins in the blood."

Zuckermann is a pioneer in the development of peptoids, synthetic polymers that behave like naturally occurring proteins but can withstand aggressive chemical and biological environments without degrading. His group previously discovered peptoids capable of self-assembling into nanoscale jaws, nanosheets, and nanoscale ropes that braid themselves.

"Peptoids are ideal for this application as they are similar to proteins in structure, but different enough that they aren't degraded by enzymes in the blood," added Zuckermann. "We can now engineer materials that are capable of specific recognition yet can evade destruction."

Using the Foundry's state-of-the-art robotic synthesis capabilities, the team prepared a panel of peptoids designed to capture a misfolded prion protein, an abnormal, infectious form of a cellular protein found in the brain. By attaching these peptoids to tiny magnetic beads, the team could then use a magnet to isolate misfolded proteins directly from blood samples. The most selective and sensitive of these peptoids, coined aggregate-specific reagent, or ASR1, could capture not only the prion aggregates, but aggregates associated with Alzheimer's disease as well.

"Our study shows how basic research capabilities can be translated into a practical application," said Zuckermann. "The potential for this tool to serve as a diagnostic in other misfolded protein diseases, such as Parkinson's and Type II diabetes, is wide open, and I'm excited to continue this collaboration."

This research is reported in a paper titled, "A universal method for detection of amyloidogenic misfolded proteins," appearing in the journal Biochemistry and available in Biochemistry online. Co-authoring the paper with Zuckermann were Cleo Salisbury, Xuemei Wang, Carol Gao, Michael Connolly, Thieu Bleu, John Hall, Joseph Fedynyshyn, Sophie Allauzen and David Peretz.

Portions of this work were supported by DOE's Office of Science.

The Molecular Foundry is one of the five DOE Nanoscale Science Research Centers (NSRCs), premier national user facilities for interdisciplinary research at the nanoscale. Together the NSRCs comprise a suite of complementary facilities that provide researchers with state-of-the-art capabilities to fabricate, process, characterize and model nanoscale materials, and constitute the largest infrastructure investment of the National Nanotechnology Initiative. The NSRCs are located at DOE's Argonne, Brookhaven, Lawrence Berkeley, Oak Ridge and Sandia and Los Alamos National Laboratories. For more information about the DOE NSRCs, please visit nano.energy.gov.

####

About Berkeley Lab
Lawrence Berkeley National Laboratory addresses the world’s most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab’s scientific expertise has been recognized with 12 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy’s Office of Science. For more, visit www.lbl.gov.

For more information, please click here

Contacts:
Aditi Risbud
(510)486-4861

Copyright © Berkeley Lab

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Pixel-array quantum cascade detector paves the way for portable thermal imaging devices: Research team from TU-Wien Center for Micro- and Nanostructures have developed a new 'cooler' sensing instrument thereby increasing energy-efficiency and enhancing mobility for diagnostic tes July 28th, 2016

Dirty to drinkable: Engineers develop novel hybrid nanomaterials to transform water July 28th, 2016

Thomas Swan and NGI announce unique partnership July 28th, 2016

Penn team uses nanoparticles to break up plaque and prevent cavities July 28th, 2016

Laboratories

New nontoxic process promises larger ultrathin sheets of 2-D nanomaterials July 27th, 2016

New lithium-oxygen battery greatly improves energy efficiency, longevity: New chemistry could overcome key drawbacks of lithium-air batteries July 26th, 2016

An accelerated pipeline to open materials research: ORNL workflow system unites imaging, algorithms, and HPC to advance materials discovery and design July 24th, 2016

Scientists develop way to upsize nanostructures into light, flexible 3-D printed materials: Virginia Tech, Livermore National Lab researchers develop hierarchical 3-D printed metallic materials July 20th, 2016

Govt.-Legislation/Regulation/Funding/Policy

Thomas Swan and NGI announce unique partnership July 28th, 2016

Penn team uses nanoparticles to break up plaque and prevent cavities July 28th, 2016

Beating the heat a challenge at the nanoscale: Rice University scientists detect thermal boundary that hinders ultracold experiments July 28th, 2016

Enhancing molecular imaging with light: New technology platform increases spectroscopic resolution by 4 fold July 27th, 2016

Nanomedicine

Pixel-array quantum cascade detector paves the way for portable thermal imaging devices: Research team from TU-Wien Center for Micro- and Nanostructures have developed a new 'cooler' sensing instrument thereby increasing energy-efficiency and enhancing mobility for diagnostic tes July 28th, 2016

Starpharma initiates new DEP™ drug delivery program with AstraZeneca July 27th, 2016

Scientists test nanoparticle drug delivery in dogs with osteosarcoma July 26th, 2016

The NanoWizard® AFM from JPK is applied for interdisciplinary research at the University of South Australia for applications including smart wound healing and how plants can protect themselves from toxins July 26th, 2016

Discoveries

Pixel-array quantum cascade detector paves the way for portable thermal imaging devices: Research team from TU-Wien Center for Micro- and Nanostructures have developed a new 'cooler' sensing instrument thereby increasing energy-efficiency and enhancing mobility for diagnostic tes July 28th, 2016

Dirty to drinkable: Engineers develop novel hybrid nanomaterials to transform water July 28th, 2016

Penn team uses nanoparticles to break up plaque and prevent cavities July 28th, 2016

Beating the heat a challenge at the nanoscale: Rice University scientists detect thermal boundary that hinders ultracold experiments July 28th, 2016

Announcements

Pixel-array quantum cascade detector paves the way for portable thermal imaging devices: Research team from TU-Wien Center for Micro- and Nanostructures have developed a new 'cooler' sensing instrument thereby increasing energy-efficiency and enhancing mobility for diagnostic tes July 28th, 2016

Dirty to drinkable: Engineers develop novel hybrid nanomaterials to transform water July 28th, 2016

Thomas Swan and NGI announce unique partnership July 28th, 2016

Penn team uses nanoparticles to break up plaque and prevent cavities July 28th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic