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Home > Press > New PCR reagent kit accelerates next-generation sequencing

Abstract:
Available exclusively in the UK from Cambio, the new HaloPlex™ Target Enrichment Kit revolutionises next-generation sequencing, reducing costs and saving up to 80 per cent in sample preparation time compared to many other kits.

New PCR reagent kit accelerates next-generation sequencing

Cambridge, UK | Posted on May 27th, 2011

HaloPlex Target Enrichment Kit introduces a dramatically improved PCR technology that enables millions of PCR reactions in a single tube. Following fragmentation using specifically selected restriction endonucleases, and denaturing of the DNA sample, a Selector Probe library is added. Each probe, designed to hybridize to both ends of a targeted DNA restriction fragment, guides the targeted fragments to form circular DNA molecules. Only circular DNA targets are amplified, ready for sequencing using any next-generation procedure, and results are highly reproducible.

According to Dr Naeem Ahmed, Cambio's technical support specialist, ‘This new Halo Genomics kit provides a "lab-in-a-tube" solution which greatly simplifies PCR workflow, with no need for expensive instrumentation or automation. With next-generation nano scale PCR, reactions are parallelised at the molecular level so that no separate time-consuming library preparation stage is required. For a project targeting 100 genes in 1000 samples, HaloPlex Target Enrichment Kit reduces the total sample preparation time by as much as 80 per cent. Genomic regions of interest are enriched at the same time as the introduction of sample barcoding and sequence motifs.'

Current applications demonstrating the value of the HaloPlex PCR reagent kit for high performance targeted resequencing include studies of candidate genes involved in rare inherited diseases, pathway sequencing in matched tumour and normal samples, and clinical sequencing for BRCA gene assays.

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About Cambio
Established in 1985, Cambio is a privately-held UK company with an excellent reputation for the supply of high quality molecular biology reagents and consumables. To meet the needs of a growing and diversifying market, advanced Molecular Biology products are continually added to the company’s extensive portfolio. This wide choice of research tools sourced from a global network of leading manufacturers is complemented by Cambio’s commitment to world-class technical support and customer service.

As a result, the company has built successful long-term customer relationships with scientific research laboratories within universities, large pharma and biotech companies, government agencies, agricultural organisations and environmental facilities. Based near Cambridge, UK, Cambio is ISO9001:2008 registered.

For more information, please click here

Contacts:
For sales and technical information please contact:
Cambio Ltd
The Irwin Centre, Scotland Road
Dry Drayton, Cambridge, CB23 8AR
Tel: 01954 210200
Fax: 01954 210300
E-mail:

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