Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > The University of St Andrews chooses NanoSight NTA system for exosomes characterization

Dr Simon Powis with his NanoSight LM10 system to study exosome behaviour
Dr Simon Powis with his NanoSight LM10 system to study exosome behaviour

Abstract:
NanoSight, world-leading manufacturers of unique nanoparticle characterization technology reports that the School of Medicine at the University of St Andrews is using nanoparticle tracking analysis, NTA, to characterize exosome behaviour.

The University of St Andrews chooses NanoSight NTA system for exosomes characterization

Salisbury, UK | Posted on May 10th, 2011

Dr Simon Powis and his colleagues at the University of St Andrews are working to understand how a set of molecules involved in the immune system's defence against intracellular pathogens function. These molecules are called major histocompatibility complex (MHC) class I molecules, and they are expressed on almost every cell in the body. Their relevance to medicine is most commonly known because they are one of the key sets of genes that have to be closely matched when an organ transplant is made, otherwise the transplant can be rejected. It is now known that their precise role in the normal immune system is to bind small fragments of degraded viral proteins which they display to T lymphocytes of the immune system. This allows the specific detection of 'foreign' proteins, i.e. from potential pathogens, and allows the immune system to specifically detect and kill infected cells, whilst leaving a neighbouring uninfected cell alone. In addition, there is one fascinating autoimmune disease closely associated with a particular type of MHC class I molecule. Over 90% of patients with a type of inflammatory arthritis called ankylosing spondylitis which affects the spine, expresses one specific type of MHC class I molecule called HLA-B27. The link between this arthritic condition and HLA-B27 has been known for almost 40 years, but the disease mechanism and how HLA-B27 is involved is yet to be understood.

Whilst the Powis group were studying MHC class I molecules expressed on exosomes, it was discovered that they can express a novel type of structure. The tail region of the MHC class I molecule, which sits inside the exosome, can frequently form a disulfide-bond linkage to another MHC class I molecule, thus bringing two molecules closely together in a dimeric structure. This normally does not happen on cells because the cytoplasm has a reducing environment, preventing disulfide bonds from forming. However, in exosomes the capacity to maintain a reducing environment seems to have been lost. The group is now studying whether cells of the immune system see these MHC class I dimers structures on exosomes and respond to them. Another key question is what peptides are found bound to MHC class I molecules on exosomes. The exosome production pathway is not the normal route for MHC class I molecules to get to the cell surface, so the possibility that different peptides are found in this subset of exosomal MHC class I molecules is a real possibility. To be able to study these exosomes from a variety of immune cells, it is necessary to detect their presence and size in cultures. This is the reason for the team choosing the NanoSight NTA approach with the LM10 system.

Prior to using NanoSight, flow cytometry had proved a valuable tool in the preliminary characterisation of the exosomes released by immune cells. Dr Powis says "The NanoSight approach allows a more accurate determination of size and relative concentration both before and after purification. This allows us to monitor the release of exosomes in the range 30-150nm after activation with a variety of immune stimuli relevant to both normal and aberrant immune responses in a way not previously visible with flow cytometry."

To learn more about nanoparticle characterization using Nanoparticle Tracking Analysis, NTA, please visit the company website (www.nanosight.com) and register for the latest issue of NanoTrail, the company's electronic newsletter.

####

About NanoSight
NanoSight delivers the world’s most versatile and proven multi-parameter nanoparticle analysis in a single instrument.

NanoSight visualizes, measures and characterizes virtually all nanoparticles. Particle size, concentration, Zeta potential and aggregation can all be analyzed while a fluorescence mode provides differentiation of labelled particles. NanoSight presents real time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses uniquely confirmed by visual validation.

NanoSight’s “Nanoparticle Tracking Analysis” (NTA) detects and visualizes populations of nanoparticles in liquids down to 10nm, dependent on material, and measures the size of each particle from direct observations of diffusion. This particle-by-particle methodology goes beyond traditional light scattering and other ensemble techniques in providing high-resolution particle size distributions. Additionally, NanoSight measures concentration and validates data with information-rich video files of the particles moving under Brownian motion.

NanoSight’s comprehensive characterization matches the demands of complex biological systems, hence its wide application in development of drug delivery systems, of viral vaccines, in nanotoxicology and in biodiagnostics. This real-time data gives insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner.

NanoSight has a growing role in biodiagnostics, being proven in detection and speciation of nanovesicles (exosomes) and microvesicles. As functionalized nanoparticles increasingly fulfill their potential in biodiagnostics, NanoSight is ever more the analytical platform of choice.

NanoSight demonstrates worldwide success through rapid adoption of NTA, having installed more than 300 systems worldwide with users including BASF, GlaxoSmithKline, Merck, Novartis, Pfizer, Proctor and Gamble, Roche and Unilever together with the most eminent universities and research institutes. In addition to this user base more than 150 third party papers citing NanoSight results consolidate NanoSight’s leadership position in nanoparticle characterization. For more information, visit the NanoSight website (www.nanosight.com).

For more information, please click here

Contacts:
NanoSight Limited
Minton Park
London Road
Amesbury SP4 7RT
T +44 (0) 1980 676060
F +44 (0) 1980 624703


NetDyaLog Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA
T +44 (0) 1799 521881
M +44 (0) 7843 012997

Copyright © NanoSight

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Protein Building Blocks for Nanosystems: Scientists develop method for producing bio-based materials with new properties April 17th, 2015

Oxford Instruments commissions high field outsert magnet system for the National High Magnetic Field Laboratory 32 Tesla magnet program April 17th, 2015

QD Vision Expands Product Line with Two-Millimeter Color LCD Display Optic: Color IQ™ Optic Enables Full-Color Gamut for Ultra-Thin Displays and All-in-One Computers April 16th, 2015

The National Science Foundation names engineering researcher Andrea Alú its Alan T. Waterman awardee for 2015: Alú is a pioneer in the field of metamaterials who has developed "cloaking" technology to make objects invisible to sensors April 16th, 2015

Imaging

Combined effort for structural determination April 15th, 2015

JPK reports on the use of the NanoWizard® 3 AFM system at the Hebrew University of Jerusalem April 14th, 2015

Deben reports on the research of Dr Sunita Ho from UCSF using a CCT500 tensile stage to study the behaviour of dental materials April 14th, 2015

The Casiraghi Group, located at the University of Manchester's NanoScience and Spectroscopy Laboratory, use Raman in the study of graphene April 14th, 2015

Announcements

Protein Building Blocks for Nanosystems: Scientists develop method for producing bio-based materials with new properties April 17th, 2015

Oxford Instruments commissions high field outsert magnet system for the National High Magnetic Field Laboratory 32 Tesla magnet program April 17th, 2015

Newly-Developed Nanocatalysts Increase Performance of Fuel Cells April 16th, 2015

Lanthanide-Organic Framework Nanothermometers Prepared by Spray-Drying April 16th, 2015

Tools

Oxford Instruments commissions high field outsert magnet system for the National High Magnetic Field Laboratory 32 Tesla magnet program April 17th, 2015

Lanthanide-Organic Framework Nanothermometers Prepared by Spray-Drying April 16th, 2015

Combined effort for structural determination April 15th, 2015

JPK reports on the use of the NanoWizard® 3 AFM system at the Hebrew University of Jerusalem April 14th, 2015

New-Contracts/Sales/Customers

Oxford Instruments commissions high field outsert magnet system for the National High Magnetic Field Laboratory 32 Tesla magnet program April 17th, 2015

JPK reports on the use of the NanoWizard® 3 AFM system at the Hebrew University of Jerusalem April 14th, 2015

Deben reports on the research of Dr Sunita Ho from UCSF using a CCT500 tensile stage to study the behaviour of dental materials April 14th, 2015

UK National Graphene Institute Selects Bruker as Official Partner: World-Leading Graphene Research Facility Purchases Multiple Bruker AFMs April 7th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE