Nanotechnology Now





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > The University of Leipzig adds JPK's CellHesion 200 capability to enhance cell-cell force measurements

Steve Pawlizak, a post graduate student in Professor Käs' group at the University of Leipzig using the JPK CellHesion 200 system.
Steve Pawlizak, a post graduate student in Professor Käs' group at the University of Leipzig using the JPK CellHesion 200 system.

Abstract:
JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, reports that the University of Leipzig has chosen the CellHesion® 200 system for their Institute of Experimental Physics I.

The University of Leipzig adds JPK's CellHesion 200 capability to enhance cell-cell force measurements

Berlin, Germany | Posted on May 4th, 2011

The research of the Institute of Experimental Physics I is focused on soft condensed matter as bulk material, in interaction with surfaces and interfaces, and with single molecules. The systems under study cover a wealth like small tracer molecules, liquid crystals, polymers, polymer-networks, proteins, and even living biological cells. It is objective of the research of the Institute of Experimental Physics I to explore the physical basis of structure-property relationships in these systems.

Professor Josef A. Käs moved his group to Leipzig in 2001 from the University of Texas at Austin. At this time, he became one of the first users of the JPK NanoWizard® series of atomic force microscopes to start a close series of interactions between his group and JPK. Most recently, Professor Käs added the JPK CellHesion 200 system to provide the ability to study the interplay between compartmentalization of cell and tumor spreading.

Compartmentalization is the formation of cellular compartments (e.g. tissues and organs). It generates well-defined boundaries for various differentiated cell types. Cells of the same type adhere better to each other, whereas mixtures of different migrating cell types segregate. According to the differential adhesion hypothesis (Malcom S. Steinberg, 1960s), cell sorting and formation of cellular compartments result from different adhesiveness of participating cells. The group tries to apply and verify the concept of compartmentalization and differential adhesion hypothesis to tumor development and spreading. It is known that young tumor cells are confined to their compartment of origin. With rising malignancy up to metastasis, tumor cells become able to overcome compartment boundaries. The goal is to clarify whether tumor stages can be characterized by cellular adhesiveness. This is why they are measuring healthy and cancerous cells of different malignancy with the JPK CellHesion 200.

Another project applying CellHesion 200 is one studying biocompatibility. Magnetic shape memory alloys are a class of smart materials which have a high potential for actuators in biomedical applications. These are tested for their biocompatibility by coating those materials with different cell adhesion proteins and using the CellHesion 200 for cell-substrate adhesion measurements.

JPK's CellHesion 200 system is a dedicated stand-alone platform for cell adhesion and cytomechanics studies to be used with inverted optical or confocal microscopes. It enables the quantification of single cell-cell and cell-surface interactions under physiological conditions. This ground-breaking technique, known as single cell force spectroscopy (SCFS), measures the interaction forces between a living cell bound to a cantilever and a target cell, functionalized substrate or biomaterial. In parallel, cytomechanical characteristics including stiffness and elasticity can be determined. Data can be measured for a number of important parameters involved in cellular adhesion, including maximum cell adhesion force, single unbinding events, tether characteristics, and work of removal.

Choosing to work with JPK has proved very beneficial for the Käs group. Speaking on behalf of the group, post graduate student Steve Pawlizak says "In our opinion, JPK offers the best SFM solution for biological or biophysical application available on the market. In a convenient way, it enables simultaneous use of SFM and a variety of light microscopy techniques such as bright field, phase contrast, epi-fluorescence as well as laser scanning microscopy on inverted research microscopes. This is absolutely necessary for our applications in cellular biophysics."

####

About JPK Instruments (JPK)
JPK Instruments AG is a world-leading manufacturer of nanoanalytic instruments - particularly atomic force microscope (AFM) systems and optical tweezers - for a broad range of applications reaching from soft matter physics to nano-optics, from surface chemistry to cell and molecular biology. From its earliest days applying atomic force microscope (AFM) technology, JPK has recognized the opportunities provided by nanotechnology for transforming life sciences and soft matter research. This focus has driven JPK’s success in uniting the worlds of nanotechnology tools and life science applications by offering cutting-edge technology and unique applications expertise. Headquartered in Berlin and with direct operations in Dresden, Cambridge (UK), Singapore, Tokyo (Japan) and Paris (France), JPK maintains a global network of distributors and support centers and provides on the spot applications and service support to an ever-growing community of researchers.

For more information, please click here

Contacts:
Jezz Leckenby
NetDyaLog Limited
T: +44 (0) 1799 521881
M: +44 (0) 7843 012997
or
Claudia Boettcher
JPK Instruments
T: +49 (0) 30 5331 12070

Copyright © JPK Instruments (JPK)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Chitosan coated, chemotherapy packed nanoparticles may target cancer stem cells June 30th, 2015

BASF and Fraunhofer IPMS-CNT jointly develop electronic materials June 30th, 2015

Researchers from the UCA, key players in a pioneering study that may explain the origin of several digestive diseases June 30th, 2015

Oxford Instruments’ TritonXL Cryofree dilution refrigerator selected for the Oxford NQIT Quantum Technology Hub project June 30th, 2015

Imaging

How Graphene–based Nanomaterials and Films Revolutionize Science Explained in July 9 Webinar Hosted by Park Systems June 29th, 2015

Keysight Technologies Introduces Ultrafast-Scanning 9500 Atomic Force Microscope: New Integrated Software, Hardware Delivers Unmatched Scan Rates June 29th, 2015

Rice University boots up powerful microscopes: New electron microscopes will capture images at subnanometer resolution June 29th, 2015

X-rays and electrons join forces to map catalytic reactions in real-time: New technique combines electron microscopy and synchrotron X-rays to track chemical reactions under real operating conditions June 29th, 2015

Announcements

BASF and Fraunhofer IPMS-CNT jointly develop electronic materials June 30th, 2015

Graphene flexes its electronic muscles: Rice-led researchers calculate electrical properties of carbon cones, other shapes June 30th, 2015

Researchers from the UCA, key players in a pioneering study that may explain the origin of several digestive diseases June 30th, 2015

Oxford Instruments’ TritonXL Cryofree dilution refrigerator selected for the Oxford NQIT Quantum Technology Hub project June 30th, 2015

Tools

Oxford Instruments’ TritonXL Cryofree dilution refrigerator selected for the Oxford NQIT Quantum Technology Hub project June 30th, 2015

How Graphene–based Nanomaterials and Films Revolutionize Science Explained in July 9 Webinar Hosted by Park Systems June 29th, 2015

Keysight Technologies Introduces Ultrafast-Scanning 9500 Atomic Force Microscope: New Integrated Software, Hardware Delivers Unmatched Scan Rates June 29th, 2015

Rice University boots up powerful microscopes: New electron microscopes will capture images at subnanometer resolution June 29th, 2015

New-Contracts/Sales/Customers

Oxford Instruments’ TritonXL Cryofree dilution refrigerator selected for the Oxford NQIT Quantum Technology Hub project June 30th, 2015

Centre for Process Innovation pilots Beneq’s breakthrough roll-to-roll ALD system for moisture barrier films June 3rd, 2015

Argonne chooses Beneq’s TFS 500 Atomic Layer Deposition System: Modularity and flexibility make for a natural choice May 14th, 2015

New JEOL E-Beam Lithography System to Enhance Quantum NanoFab Capabilities May 6th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project