Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > The University of Leipzig adds JPK's CellHesion 200 capability to enhance cell-cell force measurements

Steve Pawlizak, a post graduate student in Professor Käs' group at the University of Leipzig using the JPK CellHesion 200 system.
Steve Pawlizak, a post graduate student in Professor Käs' group at the University of Leipzig using the JPK CellHesion 200 system.

Abstract:
JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, reports that the University of Leipzig has chosen the CellHesion® 200 system for their Institute of Experimental Physics I.

The University of Leipzig adds JPK's CellHesion 200 capability to enhance cell-cell force measurements

Berlin, Germany | Posted on May 4th, 2011

The research of the Institute of Experimental Physics I is focused on soft condensed matter as bulk material, in interaction with surfaces and interfaces, and with single molecules. The systems under study cover a wealth like small tracer molecules, liquid crystals, polymers, polymer-networks, proteins, and even living biological cells. It is objective of the research of the Institute of Experimental Physics I to explore the physical basis of structure-property relationships in these systems.

Professor Josef A. Käs moved his group to Leipzig in 2001 from the University of Texas at Austin. At this time, he became one of the first users of the JPK NanoWizard® series of atomic force microscopes to start a close series of interactions between his group and JPK. Most recently, Professor Käs added the JPK CellHesion 200 system to provide the ability to study the interplay between compartmentalization of cell and tumor spreading.

Compartmentalization is the formation of cellular compartments (e.g. tissues and organs). It generates well-defined boundaries for various differentiated cell types. Cells of the same type adhere better to each other, whereas mixtures of different migrating cell types segregate. According to the differential adhesion hypothesis (Malcom S. Steinberg, 1960s), cell sorting and formation of cellular compartments result from different adhesiveness of participating cells. The group tries to apply and verify the concept of compartmentalization and differential adhesion hypothesis to tumor development and spreading. It is known that young tumor cells are confined to their compartment of origin. With rising malignancy up to metastasis, tumor cells become able to overcome compartment boundaries. The goal is to clarify whether tumor stages can be characterized by cellular adhesiveness. This is why they are measuring healthy and cancerous cells of different malignancy with the JPK CellHesion 200.

Another project applying CellHesion 200 is one studying biocompatibility. Magnetic shape memory alloys are a class of smart materials which have a high potential for actuators in biomedical applications. These are tested for their biocompatibility by coating those materials with different cell adhesion proteins and using the CellHesion 200 for cell-substrate adhesion measurements.

JPK's CellHesion 200 system is a dedicated stand-alone platform for cell adhesion and cytomechanics studies to be used with inverted optical or confocal microscopes. It enables the quantification of single cell-cell and cell-surface interactions under physiological conditions. This ground-breaking technique, known as single cell force spectroscopy (SCFS), measures the interaction forces between a living cell bound to a cantilever and a target cell, functionalized substrate or biomaterial. In parallel, cytomechanical characteristics including stiffness and elasticity can be determined. Data can be measured for a number of important parameters involved in cellular adhesion, including maximum cell adhesion force, single unbinding events, tether characteristics, and work of removal.

Choosing to work with JPK has proved very beneficial for the Käs group. Speaking on behalf of the group, post graduate student Steve Pawlizak says "In our opinion, JPK offers the best SFM solution for biological or biophysical application available on the market. In a convenient way, it enables simultaneous use of SFM and a variety of light microscopy techniques such as bright field, phase contrast, epi-fluorescence as well as laser scanning microscopy on inverted research microscopes. This is absolutely necessary for our applications in cellular biophysics."

####

About JPK Instruments (JPK)
JPK Instruments AG is a world-leading manufacturer of nanoanalytic instruments - particularly atomic force microscope (AFM) systems and optical tweezers - for a broad range of applications reaching from soft matter physics to nano-optics, from surface chemistry to cell and molecular biology. From its earliest days applying atomic force microscope (AFM) technology, JPK has recognized the opportunities provided by nanotechnology for transforming life sciences and soft matter research. This focus has driven JPK’s success in uniting the worlds of nanotechnology tools and life science applications by offering cutting-edge technology and unique applications expertise. Headquartered in Berlin and with direct operations in Dresden, Cambridge (UK), Singapore, Tokyo (Japan) and Paris (France), JPK maintains a global network of distributors and support centers and provides on the spot applications and service support to an ever-growing community of researchers.

For more information, please click here

Contacts:
Jezz Leckenby
NetDyaLog Limited
T: +44 (0) 1799 521881
M: +44 (0) 7843 012997
or
Claudia Boettcher
JPK Instruments
T: +49 (0) 30 5331 12070

Copyright © JPK Instruments (JPK)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

SEMATECH to Showcase Innovation and Advances in Manufacturing at SEMICON Japan 2014: SEMATECH experts will share the latest techniques, emerging trends and best practices in advanced manufacturing strategies and methodologies November 26th, 2014

Australian startup creates world’s first 100% cotton hydrophobic T-Shirts November 26th, 2014

The mysterious 'action at a distance' between liquid containers November 26th, 2014

'Giant' charge density disturbances discovered in nanomaterials: Juelich researchers amplify Friedel oscillations in thin metallic films November 26th, 2014

Research yields material made of single-atom layers that snap together like Legos November 25th, 2014

Imaging

Renishaw receives Queen's Award for spectroscopy developments November 25th, 2014

A*STAR SIMTech wins international award for breaking new ground in actuators: SIMTech invention can be used in an array of industries, and is critical for next generation ultra-precision systems November 24th, 2014

Professional AFM Images with a Three Step Click SmartScan by Park Systems Revolutionizes Atomic Force Microscopy by Automatizing the Imaging Process November 24th, 2014

Announcements

SEMATECH to Showcase Innovation and Advances in Manufacturing at SEMICON Japan 2014: SEMATECH experts will share the latest techniques, emerging trends and best practices in advanced manufacturing strategies and methodologies November 26th, 2014

Australian startup creates world’s first 100% cotton hydrophobic T-Shirts November 26th, 2014

The mysterious 'action at a distance' between liquid containers November 26th, 2014

'Giant' charge density disturbances discovered in nanomaterials: Juelich researchers amplify Friedel oscillations in thin metallic films November 26th, 2014

Tools

Renishaw receives Queen's Award for spectroscopy developments November 25th, 2014

JPK reports on the use of AFM and the CellHesion module to study plant cells at the University of Queensland November 25th, 2014

A*STAR SIMTech wins international award for breaking new ground in actuators: SIMTech invention can be used in an array of industries, and is critical for next generation ultra-precision systems November 24th, 2014

Professional AFM Images with a Three Step Click SmartScan by Park Systems Revolutionizes Atomic Force Microscopy by Automatizing the Imaging Process November 24th, 2014

New-Contracts/Sales/Customers

Iran Exports Nanodrugs to Syria November 24th, 2014

Tesla NanoCoatings Increasing Use of SouthWest NanoTechnologies Carbon Nanotubes (CNTs) for its Infrastructure Coatings and Paints: High Quality SMW™ Specialty Multi-wall Carbon Nanotubes Incorporated into Teslan®-brand coatings used by Transportation, Oil and Gas Companies November 19th, 2014

Artificial retina could someday help restore vision November 12th, 2014

Lomiko Signs Licensing Agreement to Produce and Supply Power Converter Systems to E-Commerce Customers October 29th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More












ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE