Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > With Chemical Modification, Stable RNA Nanoparticles Go 3-D

Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair in biomedical engineering with students in his lab at the Vontz Center for Molecular Studies
Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair in biomedical engineering with students in his lab at the Vontz Center for Molecular Studies

Abstract:
Researchers have found a way to bypass RNase and create stable three-dimensional configurations of RNA

With Chemical Modification, Stable RNA Nanoparticles Go 3-D

Cincinnati, OH | Posted on February 14th, 2011

For years, RNA has seemed an elusive tool in nanotechnology research—easily manipulated into a variety of structures, yet susceptible to quick destruction when confronted with a commonly found enzyme.

"The enzyme RNase cuts RNA randomly into small pieces, very efficiently and within minutes," explains Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair and professor of biomedical engineering at the University of Cincinnati (UC). "Moreover, RNase is present everywhere, making the preparation of RNA in a lab extremely difficult."

But by replacing a chemical group in the macromolecule, Guo says he and fellow researchers have found a way to bypass RNase and create stable three-dimensional configurations of RNA, greatly expanding the possibilities for RNA in nanotechnology (the engineering of functional systems at the molecular scale).

Their results, "Fabrication of Stable and RNase-Resistant RNA Nanoparticles Active in Gearing the Nanomotors for Viral DNA Packaging," are published online in the journal ACS Nano.

In their work, Guo and his colleagues focused on the ribose rings that, together with alternating phosphate groups, form the backbone of RNA. By changing one section of the ribose ring, Guo and his team altered the structure of the molecule, making it unable to bind with RNase and able to resist degradation.

"RNase interaction with RNA requires a match of structural conformation," says Guo. "When RNA conformation has changed, the RNase cannot recognize RNA and the binding becomes an issue."

While he says previous researchers have shown this alteration makes RNA stable in a double helix, they did not study its potential to affect the folding of RNA into a three-dimensional structure necessary for nanotechnology.

After creating the RNA nanoparticle, Guo and his colleagues successfully used it to power the DNA packaging nanomotor of bacteriophage phi29, a virus that infects bacteria.

"We found that the modified RNA can fold into its 3-D structure appropriately, and can carry out its biological functions after modification," says Guo. "Our results demonstrate that it is practical to produce RNase-resistant, biologically active, and stable RNA for application in nanotechnology."

Because stable RNA molecules can be used to assemble a variety of nanostructures, Guo says they are an ideal tool to deliver targeted therapies to cancerous or viral-infected cells:

"RNA nanoparticles can be fabricated with a level of simplicity characteristic of DNA while possessing versatile structure and catalytic function similar to that of proteins. With this RNA modification, hopefully we can open new avenues of study in RNA nanotechnology."

Guo serves as director of UC's National Institutes of Health (NIH) Nanomedicine Development Center and Nanobiomedical Center. This work was funded by grants from the NIH.

Co-authors include Jing Liu, Mathieu Cinier and Yi Shu from UC, Chaoping Chen from Colorado State University, Guanxin Shen from Huazhong University of Science and Technology in China, and Songchuan Guo from Kylin Therapeutics, Inc.

####

For more information, please click here

Contacts:
Katy Cosse

513-558-0207
University of Cincinnati Academic Health Center

Copyright © University of Cincinnati

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Cool Calculations for Cold Atoms: New theory of universal three-body encounters September 2nd, 2014

Accounting for Biological Aggregation in Heating and Imaging of Magnetic Nanoparticles September 2nd, 2014

Engineers develop new sensor to detect tiny individual nanoparticles September 2nd, 2014

Future solar panels September 2nd, 2014

Govt.-Legislation/Regulation/Funding/Policy

Cool Calculations for Cold Atoms: New theory of universal three-body encounters September 2nd, 2014

UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique: Oil-and-water approach from Richmond's UO lab to spark new line of versatile peptoid nanosheets September 2nd, 2014

Accounting for Biological Aggregation in Heating and Imaging of Magnetic Nanoparticles September 2nd, 2014

Engineers develop new sensor to detect tiny individual nanoparticles September 2nd, 2014

Possible Futures

Air Force’s 30-year plan seeks 'strategic agility' August 1st, 2014

IBM Announces $3 Billion Research Initiative to Tackle Chip Grand Challenges for Cloud and Big Data Systems: Scientists and engineers to push limits of silicon technology to 7 nanometers and below and create post-silicon future July 10th, 2014

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

Local girl does good March 22nd, 2014

Academic/Education

New Vice President Takes Helm at CNSE CMOST: Catherine Gilbert To Lead CNSE Children’s Museum of Science and Technology Through Expansion And Relocation August 29th, 2014

RMIT delivers $30m boost to micro and nano-tech August 26th, 2014

SEMATECH and Newly Merged SUNY CNSE/SUNYIT Launch New Patterning Center to Further Advance Materials Development: Center to Provide Access to Critical Tools that Support Semiconductor Technology Node Development August 7th, 2014

Oxford Instruments Asylum Research and the Center for Nanoscale Systems at Harvard University Present a Workshop on AFM Nanomechanical and Nanoelectrical Characterization, Aug. 21-22 August 6th, 2014

Nanomedicine

UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique: Oil-and-water approach from Richmond's UO lab to spark new line of versatile peptoid nanosheets September 2nd, 2014

Accounting for Biological Aggregation in Heating and Imaging of Magnetic Nanoparticles September 2nd, 2014

Nano-forests to reveal secrets of cells September 2nd, 2014

Nanoscale assembly line August 29th, 2014

Announcements

New synthesis method may shape future of nanostructures, clean energy: Findings advance efficient solar spliting of water into hydrogen fuel September 2nd, 2014

Accounting for Biological Aggregation in Heating and Imaging of Magnetic Nanoparticles September 2nd, 2014

Engineers develop new sensor to detect tiny individual nanoparticles September 2nd, 2014

Future solar panels September 2nd, 2014

Nanobiotechnology

UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique: Oil-and-water approach from Richmond's UO lab to spark new line of versatile peptoid nanosheets September 2nd, 2014

Nano-forests to reveal secrets of cells September 2nd, 2014

Nanoscale assembly line August 29th, 2014

The channel that relaxes DNA: Relaxing DNA strands by using nano-channels: Instructions for use August 20th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE