Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA

Micropillar array
Micropillar array

Abstract:
Panasonic, core partner within imec's Human++ program, and imec today presented at the International Electron Devices Meeting in San Francisco various critical components of a biomedical lab-on-chip sensor enabling fast detection of Single Nucleotide Polymorphisms (SNPs) in DNA, such as a miniaturized pump for on-chip generation of high pressures, a micropillar filter optimized for DNA separation achieving world-record resolution, and a SNP detector allowing on-chip detection using very small sample volumes.

Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA

San Francisco, CA | Posted on December 9th, 2010

A SNP is a single nucleotide replacement in a DNA sequence which can result in different reaction by people to pathogens and medicines. Detection of these SNP's is therefore becoming increasingly important with the move towards more personalized healthcare.

Existing methods to detect SNP's require many sample processing steps in dedicated medical tools at medical laboratories. Such tests are labor intensive, time-consuming and expensive. Moreover, rather large blood samples are needed. A lab-on-chip device can bring huge advantages both to the patient and the healthcare system. Such devices enable fast, easy-to-use, cost-effective test methods which can be performed at regular times in a doctor's office or even near the patient's bed. This is very interesting for point-of-care applications such as personalized medicine.

By combining advanced micro-electronic fabrication processes with heterogeneous integration, imec and Panasonic aim to realize a state-of-the-art microfluidic device for SNP detection. In order to do this, advanced microfluidic components have been fabricated and optimized, such as a miniaturized pump for on-chip generation of high pressure, a micropillar filter optimized for DNA separation achieving world-record resolution, and a SNP detector allowing on-chip detection using very small sample volumes.

The entrance unit of the SNP detection system samples very small volumes of blood. This entrance unit features a miniaturized high-pressure pump based on an advanced conductive polymer actuator. After optimization, the actuator generates high pressures (up to 3MPa) at low voltage (~1.5V). The high pressure is essential to generate a fluid flow through the next unit of the SNP detection system. The on-chip low voltage operation is important because it opens the path to autonomy and portability of the lab-on-chip device.

Next, the DNA separation unit featuring an advanced micro-pillar array filter was developed. This deep-UV patterned silicon pillar array was realized using advanced MEMS technology. It consists of many micron-scale pillars, being typically 20µm high and with 1-2µm inter-pillar distance. The pillar array is used for DNA separation based on ion-pair reversed-phase (IR-RP) liquid chromatography. Imec and the VUB (Vrije Universiteit Brussel), a scientific partner of imec, optimized the pillar-based IR-RP liquid chromatography technique for DNA separation. This resulted in the first miniaturized on-chip system that enables fast and highly selective separation of short, double stranded DNA strands which only differ 50 base pairs in length. The resolution of the system is the highest in the world and proves the potential to handle 5 SNPs at the same time in the final SNP detection system.

The other functional units of the SNP detector are a unit for DNA extraction and polymerase chain reaction (PCR) using heaters and temperature sensors, and a SNP detection unit based on electrochemical sensors. The miniaturization of these sensors was of crucial importance, since the minimum required sensor volume determines the blood sampling volume needed for the SNP detection, and hence the dimensions of all components of the device. Scientists of Panasonic and imec demonstrated SNP detection capabilities using on-chip sensors handling a volume as small as 0.5µL.

Imec's Human++ program provides a multidisciplinary R&D platform for industrial partners to collaborate on finding industry-relevant solutions for future healthcare and wellness needs by combining nanoelectronics and biotechnology into heterogeneous systems for diagnosis and therapy. Human++ partners build on imec's 25 years of expertise in micro- and nanoelectronics and expertise in several healthcare-relevant domains.

####

About imec
Imec performs world-leading research in nanoelectronics. Imec leverages its scientific knowledge with the innovative power of its global partnerships in ICT, healthcare and energy. Imec delivers industry-relevant technology solutions. In a unique high-tech environment, its international top talent is committed to providing the building blocks for a better life in a sustainable society.

Imec is headquartered in Leuven, Belgium, and has offices in Belgium, the Netherlands, Taiwan, US, China and Japan. Its staff of more than 1,750 people includes over 550 industrial residents and guest researchers. In 2009, imec's revenue (P&L) was 275 million euro.

Imec is a registered trademark for the activities of IMEC International (a legal entity set up under Belgian law as a "stichting van openbaar nut”), imec Belgium (imec vzw supported by the Flemish Government), imec the Netherlands (Stichting imec Nederland, part of Holst Centre which is supported by the Dutch Government), imec Taiwan (imec Taiwan Co.). and imec China (IMEC Microelectronics (Shangai) Co. Ltd.).

About Panasonic
Panasonic Corporation (based in Osaka, Japan) is a worldwide leader in the development and manufacture of electronic products for a wide range of consumer, business, and industrial needs.

About VUB
The VUB offers a quality education to more than 9000 students and hosts more than 150 research teams working on its campuses, and is one of the biggest centres of knowledge in the capital of Europe. Thanks to this expertise and its strategic location, the Vrije Universiteit Brussel is the ideal partner for prestigious research and education with an outlook on Europe and the world.The Department of Chemical Engineering of the VUB (Free University of Brussels, Belgium) aims at developing creative solutions to challenging problems in the field of separation technology and catalysis by exploiting the new possibilities in materials engineering and nanotechnology. Key to this development process is a thorough understanding of the fundamental events, from the molecular scale to the real life application level. In our group, advanced experimental techniques (lab on a chip, high-throughput experimentation, …) are combined with state-of-the-art computer modeling methods, including molecular modeling and computational fluid dynamics, to obtain insight in the fundamental adsorption, diffusion, reaction and mass transfer effects. Since 2004, the major research activity has been focusing on the design and development of perfectly ordered microfabricated support structures in separation columns. The group is the academic founder of the Flanders’ Microfluidics and Microreactor Centre.

For more information, please click here

Contacts:
Imec:
Katrien Marent
Director of External Communications
T: +32 16 28 18 80
M: +32 474 30 28 66


For Imec:
Barbara Kalkis
Maestro Marketing & PR
T: +1 408 996 9975
M: +1 408 529 4210


VUB:
Wim De Malsche
Team Leader of the Microfluidics Group at the Department of Chemical Engineering
T +32 629 37 81

Copyright © imec

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

UT Arlington researchers develop transparent nanoscintillators for radiation detection for medical safety and homeland security September 29th, 2014

Iranian Scientists Determine Grain Size, Minimize Time of Nanocomposite Synthesis September 29th, 2014

Nanoparticles Used to Improve Quality of Bone Cement September 29th, 2014

'Pixel' engineered electronics have growth potential: Rice, Oak Ridge, Vanderbilt, Penn scientists lead creation of atom-scale semiconducting composites September 29th, 2014

Nanomedicine

Nanoparticles Used to Improve Quality of Bone Cement September 29th, 2014

Teijin Aramid’s carbon nanotube fibers awarded with Paul Schlack prize: New generation super fibers bring wave of innovations to fiber market September 25th, 2014

Nanotubes help healing hearts keep the beat: Rice University, Texas Children’s Hospital patch for defects enhances electrical connections between cells September 23rd, 2014

Immune system is key ally in cyberwar against cancer: Rice University study yields new two-step strategy for weakening cancer September 23rd, 2014

Sensors

Graphene and Amaranthus Superparamagnets: Breakthrough nanoparticles discovery of Indian researcher September 23rd, 2014

IEEE International Electron Devices Meeting To Celebrate 60th Anniversary as The Leading Technical Conference for Advanced Semiconductor Devices September 18th, 2014

Biosensors Get a Boost from Graphene Partnership: $5 Million Investment Supports Dozens of Jobs and Development of 300mm Fabrication Process and Wafer Transfer Facility September 18th, 2014

The Pocket Project will develop a low-cost and accurate point-of-care test to diagnose Tuberculosis: ICN2 holds a follow-up meeting of the Project on September 18th - 19th September 18th, 2014

Discoveries

UT Arlington researchers develop transparent nanoscintillators for radiation detection for medical safety and homeland security September 29th, 2014

Iranian Scientists Determine Grain Size, Minimize Time of Nanocomposite Synthesis September 29th, 2014

Nanoparticles Used to Improve Quality of Bone Cement September 29th, 2014

'Pixel' engineered electronics have growth potential: Rice, Oak Ridge, Vanderbilt, Penn scientists lead creation of atom-scale semiconducting composites September 29th, 2014

Announcements

UT Arlington researchers develop transparent nanoscintillators for radiation detection for medical safety and homeland security September 29th, 2014

Iranian Scientists Determine Grain Size, Minimize Time of Nanocomposite Synthesis September 29th, 2014

Nanoparticles Used to Improve Quality of Bone Cement September 29th, 2014

'Pixel' engineered electronics have growth potential: Rice, Oak Ridge, Vanderbilt, Penn scientists lead creation of atom-scale semiconducting composites September 29th, 2014

Nanobiotechnology

Penn Team Studies Nanocrystals by Passing Them Through Tiny Pores September 26th, 2014

Graphene and Amaranthus Superparamagnets: Breakthrough nanoparticles discovery of Indian researcher September 23rd, 2014

New NIH/DOE Grant for Life Science Studies at NSLS-II: Funding will support operation of three powerful experimental stations designed to reveal detailed structures of proteins, viruses, and more September 23rd, 2014

Production of Organometallic Frameworks in Least Possible Time September 23rd, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE