Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA

Micropillar array
Micropillar array

Abstract:
Panasonic, core partner within imec's Human++ program, and imec today presented at the International Electron Devices Meeting in San Francisco various critical components of a biomedical lab-on-chip sensor enabling fast detection of Single Nucleotide Polymorphisms (SNPs) in DNA, such as a miniaturized pump for on-chip generation of high pressures, a micropillar filter optimized for DNA separation achieving world-record resolution, and a SNP detector allowing on-chip detection using very small sample volumes.

Breakthrough towards lab-on-chip system for fast detection of single nucleotide variations in DNA

San Francisco, CA | Posted on December 9th, 2010

A SNP is a single nucleotide replacement in a DNA sequence which can result in different reaction by people to pathogens and medicines. Detection of these SNP's is therefore becoming increasingly important with the move towards more personalized healthcare.

Existing methods to detect SNP's require many sample processing steps in dedicated medical tools at medical laboratories. Such tests are labor intensive, time-consuming and expensive. Moreover, rather large blood samples are needed. A lab-on-chip device can bring huge advantages both to the patient and the healthcare system. Such devices enable fast, easy-to-use, cost-effective test methods which can be performed at regular times in a doctor's office or even near the patient's bed. This is very interesting for point-of-care applications such as personalized medicine.

By combining advanced micro-electronic fabrication processes with heterogeneous integration, imec and Panasonic aim to realize a state-of-the-art microfluidic device for SNP detection. In order to do this, advanced microfluidic components have been fabricated and optimized, such as a miniaturized pump for on-chip generation of high pressure, a micropillar filter optimized for DNA separation achieving world-record resolution, and a SNP detector allowing on-chip detection using very small sample volumes.

The entrance unit of the SNP detection system samples very small volumes of blood. This entrance unit features a miniaturized high-pressure pump based on an advanced conductive polymer actuator. After optimization, the actuator generates high pressures (up to 3MPa) at low voltage (~1.5V). The high pressure is essential to generate a fluid flow through the next unit of the SNP detection system. The on-chip low voltage operation is important because it opens the path to autonomy and portability of the lab-on-chip device.

Next, the DNA separation unit featuring an advanced micro-pillar array filter was developed. This deep-UV patterned silicon pillar array was realized using advanced MEMS technology. It consists of many micron-scale pillars, being typically 20µm high and with 1-2µm inter-pillar distance. The pillar array is used for DNA separation based on ion-pair reversed-phase (IR-RP) liquid chromatography. Imec and the VUB (Vrije Universiteit Brussel), a scientific partner of imec, optimized the pillar-based IR-RP liquid chromatography technique for DNA separation. This resulted in the first miniaturized on-chip system that enables fast and highly selective separation of short, double stranded DNA strands which only differ 50 base pairs in length. The resolution of the system is the highest in the world and proves the potential to handle 5 SNPs at the same time in the final SNP detection system.

The other functional units of the SNP detector are a unit for DNA extraction and polymerase chain reaction (PCR) using heaters and temperature sensors, and a SNP detection unit based on electrochemical sensors. The miniaturization of these sensors was of crucial importance, since the minimum required sensor volume determines the blood sampling volume needed for the SNP detection, and hence the dimensions of all components of the device. Scientists of Panasonic and imec demonstrated SNP detection capabilities using on-chip sensors handling a volume as small as 0.5µL.

Imec's Human++ program provides a multidisciplinary R&D platform for industrial partners to collaborate on finding industry-relevant solutions for future healthcare and wellness needs by combining nanoelectronics and biotechnology into heterogeneous systems for diagnosis and therapy. Human++ partners build on imec's 25 years of expertise in micro- and nanoelectronics and expertise in several healthcare-relevant domains.

####

About imec
Imec performs world-leading research in nanoelectronics. Imec leverages its scientific knowledge with the innovative power of its global partnerships in ICT, healthcare and energy. Imec delivers industry-relevant technology solutions. In a unique high-tech environment, its international top talent is committed to providing the building blocks for a better life in a sustainable society.

Imec is headquartered in Leuven, Belgium, and has offices in Belgium, the Netherlands, Taiwan, US, China and Japan. Its staff of more than 1,750 people includes over 550 industrial residents and guest researchers. In 2009, imec's revenue (P&L) was 275 million euro.

Imec is a registered trademark for the activities of IMEC International (a legal entity set up under Belgian law as a "stichting van openbaar nut”), imec Belgium (imec vzw supported by the Flemish Government), imec the Netherlands (Stichting imec Nederland, part of Holst Centre which is supported by the Dutch Government), imec Taiwan (imec Taiwan Co.). and imec China (IMEC Microelectronics (Shangai) Co. Ltd.).

About Panasonic
Panasonic Corporation (based in Osaka, Japan) is a worldwide leader in the development and manufacture of electronic products for a wide range of consumer, business, and industrial needs.

About VUB
The VUB offers a quality education to more than 9000 students and hosts more than 150 research teams working on its campuses, and is one of the biggest centres of knowledge in the capital of Europe. Thanks to this expertise and its strategic location, the Vrije Universiteit Brussel is the ideal partner for prestigious research and education with an outlook on Europe and the world.The Department of Chemical Engineering of the VUB (Free University of Brussels, Belgium) aims at developing creative solutions to challenging problems in the field of separation technology and catalysis by exploiting the new possibilities in materials engineering and nanotechnology. Key to this development process is a thorough understanding of the fundamental events, from the molecular scale to the real life application level. In our group, advanced experimental techniques (lab on a chip, high-throughput experimentation, …) are combined with state-of-the-art computer modeling methods, including molecular modeling and computational fluid dynamics, to obtain insight in the fundamental adsorption, diffusion, reaction and mass transfer effects. Since 2004, the major research activity has been focusing on the design and development of perfectly ordered microfabricated support structures in separation columns. The group is the academic founder of the Flanders’ Microfluidics and Microreactor Centre.

For more information, please click here

Contacts:
Imec:
Katrien Marent
Director of External Communications
T: +32 16 28 18 80
M: +32 474 30 28 66


For Imec:
Barbara Kalkis
Maestro Marketing & PR
T: +1 408 996 9975
M: +1 408 529 4210


VUB:
Wim De Malsche
Team Leader of the Microfluidics Group at the Department of Chemical Engineering
T +32 629 37 81

Copyright © imec

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Measuring the Smallest Magnets July 28th, 2014

WITec to host the 11th Confocal Raman Imaging Symposium from September 29th - October 1st in Ulm, Germany July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Nanomedicine

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

New imaging agent provides better picture of the gut July 25th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Researchers create vaccine for dust-mite allergies Main Page Content: Vaccine reduced lung inflammation to allergens in lab and animal tests July 22nd, 2014

Sensors

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Compact Vibration Harvester Power Supply with Highest Efficiency Opens Door to “Fix-and-Forget” Sensor Nodes July 23rd, 2014

Nano-sized Chip "Sniffs Out" Explosives Far Better than Trained Dogs: TAU researcher's groundbreaking sensor detects miniscule concentrations of hazardous materials in the air July 23rd, 2014

Tiny laser sensor heightens bomb detection sensitivity July 19th, 2014

Discoveries

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

Measuring the Smallest Magnets July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Stanford team achieves 'holy grail' of battery design: A stable lithium anode - Engineers use carbon nanospheres to protect lithium from the reactive and expansive problems that have restricted its use as an anode July 27th, 2014

Announcements

Measuring the Smallest Magnets July 28th, 2014

WITec to host the 11th Confocal Raman Imaging Symposium from September 29th - October 1st in Ulm, Germany July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Nanobiotechnology

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Production of Non-Virus Nanocarriers with Highest Amount of Gene Delivery July 17th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE