Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Tracking Tumor-Targeting Nanoparticles in the Body

The behavior of targeted quantum dots (Qdots) across three different tumor models using intravital microscopy with submicrometer resolution is described. As in the figure, the differences in extravasation between tumor types are shown and the kinetics are quatnified. Further, by demonstrating similarity in Qdot binding to tumor blood vessels across different tumor types, this work suggests several advantages implicit in vascular targeting compared with tumor cell targeting.
The behavior of targeted quantum dots (Qdots) across three different tumor models using intravital microscopy with submicrometer resolution is described. As in the figure, the differences in extravasation between tumor types are shown and the kinetics are quatnified. Further, by demonstrating similarity in Qdot binding to tumor blood vessels across different tumor types, this work suggests several advantages implicit in vascular targeting compared with tumor cell targeting.

Abstract:
Though targeted nanoparticle-based imaging agents and therapeutics for diagnosing and treating cancer are making their way to and through the clinical trials process, researchers still do not have a good understanding of how nanoparticles reach tumors and how they then bind to and enter the targeted tumor. To overcome that knowledge deficit, two teams of investigators, both part of the Alliance for Nanotechnology in Cancer have undertaken studies aiming to track nanoparticles as they move through living animals.

Tracking Tumor-Targeting Nanoparticles in the Body

Bethesda, MD | Posted on October 27th, 2010

In one study, a team of investigators at Stanford University used quantum dots to study how nanoparticles travel through tumor blood vessels in living test subjects, bind to molecular targets on the surface of those blood vessels, and then travel out of the blood stream and into the tumor itself. Sanjiv Sam Gambhir, co-director of one of nine National Cancer Institute (NCI) Centers of Cancer Nanotechnology Excellence, led this study. He and his colleagues published their findings in the journal Small. In a second study, published in the journal ACS Nano, Alliance investigators Dong Shin, Mostafa El-Sayed, and Shuming Nie of Emory University and the Georgia Institute of Technology used targeted gold nanocrystals to study both active and passive targeting of tumors.

In the Stanford study, Dr. Gambhir and his collaborators exploited the capabilities of intravital microscopy, a technique that enables researchers to see brightly fluorescent markers through a living animal's skin in real time. In this series of experiments, the Stanford team examined nanoparticle trafficking in mice in which a variety of different types of tumors were allowed to grow in the animals' ears. For the fluorescent marker, the investigators used a near-infrared emitting quantum dot linked to RGD, a molecule known to bind tightly to a protein found on the surface of blood vessels surrounding tumors.

To their surprise, the researchers found that regardless of the type of tumor studied, nanoparticle binding only occurred when aggregates of particles - not single particles - were able to tether themselves to multiple, discreet sites within a tumor. The researchers were not able to detect any significant binding when they repeated these experiments using quantum dots lacking the RGD targeting molecule. The investigators also found that binding rates and binding patterns were consistent across all tumor types, a reassuring finding given the natural heterogeneity that characterizes human cancers.

While binding ability appears to be independent of tumor type, the same cannot be said for extravasation, i.e., the transit of a nanoparticle out of the blood stream and into a tumor. The researchers noted in their paper that it is likely that nanoparticle shape and size will play a critical role in determining how a given nanoparticle will extravasate into each particular type of tumor.

Meanwhile, the Emory-Georgia Tech team used rod-shaped gold nanocrystals linked to tumor-targeting peptides to explore the delivery mechanisms that enable nanoparticles to accumulate in tumors. The investigators used gold nanoparticles so that they could quantify the number of nanoparticles reaching tumors and other tissues. Gold does not occur naturally in mammals, so any gold detected in a given tumor or tissue using the highly sensitive and accurate technique known as elemental mass spectrometry would had to have come from gold nanoparticles.

To conduct their experiments, the investigators created three formulations by attaching one of three tumor-targeting molecules to the surface of the gold nanorods. They then injected the nanoparticles into animals bearing implanted human tumors, allowed the nanoparticles to circulate through the body, and measured the amount of gold that accumulated in the implanted tumors and other tissues. The researchers also repeated this experiment using untargeted gold nanoparticles. The results were surprising in that the targeting molecules only marginally increased the amount of gold that accumulated in tumors.

The investigators concluded that gold nanoparticles designed to be used in photothermal anticancer therapy should be injected directly into tumors rather than via intravenous administration in order to achieve the greatest concentration of gold in tumors. They also noted in their paper that these experiments suggest that target binding is not the rate limiting step for nanoparticle delivery, but rather that transport out of the blood stream and into tumors is the major barrier to nanoparticle accumulation in tumors.

The work using intravital microscopy, which is detailed in a paper titled, "Dynamic Visualization of RGD-Quantum Dot Binding to Tumor Neovasculature and Extravasation in Multiple Living Mouse Models Using Intravital Microscopy," was supported in part by the NCI Alliance for Nanotechnology in Cancer, a comprehensive initiative designed to accelerate the application of nanotechnology to the prevention, diagnosis, and treatment of cancer. An abstract of this paper is available at the journal's website.

View Abstract at dx.doi.org/doi:10.1002/smll.201001022

The work using gold nanocrystals, which is detailed in a paper titled, "A Reexamination of Active and Passive Tumor Targeting by Using Rod-Shaped Gold Nanocrystals and Covalently Targeted Peptide Ligands," was also supported in part by the NCI Alliance for Nanotechnology in Cancer. An abstract of this paper is available at the journal's website.

View abstract at pubs.acs.org/doi/abs/10.1021/nn102055s

####

About NCI Alliance for Nanotechnology in Cancer
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Copyright © NCI Alliance for Nanotechnology in Cancer

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Harris & Harris Group to Host Conference Call on Second-Quarter 2014 Financial Results on August 15, 2014 July 23rd, 2014

UCF Nanotech Spinout Developing Revolutionary Battery Technology: Power the Next Generation of Electronics with Carbon July 23rd, 2014

Deadline Announced for Registration in 7th Int'l Nanotechnology Festival in Iran July 23rd, 2014

A Crystal Wedding in the Nanocosmos July 23rd, 2014

Govt.-Legislation/Regulation/Funding/Policy

NNCO Announces an Interactive Webinar: Progress Review on the Coordinated Implementation of the National Nanotechnology Initiative 2011 Environmental, Health, and Safety Research Strategy July 23rd, 2014

Nano-sized Chip "Sniffs Out" Explosives Far Better than Trained Dogs: TAU researcher's groundbreaking sensor detects miniscule concentrations of hazardous materials in the air July 23rd, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

Penn Study: Understanding Graphene’s Electrical Properties on an Atomic Level July 22nd, 2014

Academic/Education

Haydale Announces Collaboration Agreement with Swansea University’s Welsh Centre for Printing and Coatings (WCPC) July 12th, 2014

STFC takes delivery of the 100th Hitachi Tabletop SEM in the UK July 3rd, 2014

Innovation Management and the Emergence of the Nanobiotechnology Industry July 1st, 2014

Albany NanoCollege Faculty Member Selected as Editor-in-Chief of the Prestigious Journal of Electronic Materials July 1st, 2014

Nanomedicine

Researchers create vaccine for dust-mite allergies Main Page Content: Vaccine reduced lung inflammation to allergens in lab and animal tests July 22nd, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

SentiMag® Now Available in Australia and New Zealand July 21st, 2014

More than glitter: Scientists explain how gold nanoparticles easily penetrate cells, making them useful for delivering drugs July 21st, 2014

Announcements

Harris & Harris Group to Host Conference Call on Second-Quarter 2014 Financial Results on August 15, 2014 July 23rd, 2014

UCF Nanotech Spinout Developing Revolutionary Battery Technology: Power the Next Generation of Electronics with Carbon July 23rd, 2014

Deadline Announced for Registration in 7th Int'l Nanotechnology Festival in Iran July 23rd, 2014

A Crystal Wedding in the Nanocosmos July 23rd, 2014

Quantum Dots/Rods

Researchers create quantum dots with single-atom precision June 30th, 2014

New Los Alamos Approach May Be Key to Quantum Dot Solar Cells With Real Gains in Efficiency: Nanoengineering Boosts Carrier Multiplication in Quantum Dots June 19th, 2014

MIPT-based researcher predicts new state of matter June 17th, 2014

Technology using microwave heating may impact electronics manufacture June 10th, 2014

Nanobiotechnology

Production of Non-Virus Nanocarriers with Highest Amount of Gene Delivery July 17th, 2014

Physicists Use Computer Models to Reveal Quantum Effects in Biological Oxygen Transport: The team solved a long-standing question by explaining why oxygen – and not deadly carbon monoxide – preferably binds to the proteins that transport it around the body. July 17th, 2014

Tiny DNA pyramids enter bacteria easily -- and deliver a deadly payload July 9th, 2014

Artificial cilia: Scientists from Kiel University develop nano-structured transportation system July 4th, 2014

Research partnerships

A Crystal Wedding in the Nanocosmos July 23rd, 2014

Penn Study: Understanding Graphene’s Electrical Properties on an Atomic Level July 22nd, 2014

More than glitter: Scientists explain how gold nanoparticles easily penetrate cells, making them useful for delivering drugs July 21st, 2014

Tiny laser sensor heightens bomb detection sensitivity July 19th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE