Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > All the Same Size

Swapping spheres: Monodisperse protein microspheres can be fabricated by templating on porous CaCO3 microcores (see picture; CaCO3 gray, insulin orange). The CaCO3 cores decompose upon variation of pH from 9.0 to 5.0, whereas the insulin model protein precipitates. The method requires mild conditions, no additives, and minimal processing.
Swapping spheres: Monodisperse protein microspheres can be fabricated by templating on porous CaCO3 microcores (see picture; CaCO3 gray, insulin orange). The CaCO3 cores decompose upon variation of pH from 9.0 to 5.0, whereas the insulin model protein precipitates. The method requires mild conditions, no additives, and minimal processing.

Abstract:
Assembly of uniformly pure protein microparticles using calcium carbonate templates

All the Same Size

Weinheim, Germany | Posted on October 25th, 2010

Proteins are an interesting class of drugs because they demonstrate high biological activity and are highly specific in their effects. It has become possible to produce more and more proteins with tailored pharmacological properties; however transport and controlled release of the protein drugs in the body have remained a challenge. In the journal Angewandte Chemie, Helmuth Möhwald of the Max Planck Institute of Colloids and Interfaces in Golm/Potsdam (Germany) and Dmitry V. Volodkin and Regine von Klitzing of the TU Berlin (Germany) have now introduced an alternative to the usual transport agents, such as liposomes: by using a simple, inexpensive, gentle process, they were able to produce pure protein microspheres of uniform size.

Loading nano- and microscale transport systems with proteins is the most common strategy used to bring drugs to their target area and achieve a longer period of activity. The challenge is to produce particles with a precisely defined quantity of protein, size, morphology, composition, and density. These characteristics are critical for the attainment of high bioavailability and a defined rate of release at the desired location. Unfortunately, they are difficult to control when using conventional methods for the production of protein particles, such as crystallization, spray drying, or incorporation in liposomes or polymer matrices. Another disadvantage is that these processes generally require organic solvents, high temperatures, or other conditions that can compromise the stability of the proteins.

The researchers were looking for a method that would deliver uniform protein particles without destructive additives and under mild conditions. The team has now developed such a method, which is also very simple and inexpensive, and successfully tested it on insulin, a classic therapeutic protein. The secret to their success lies in porous calcium carbonate microspheres of defined size, and a change of pH value. In a slightly alkaline aqueous environment (high pH value), the protein insulin is soluble. When the calcium carbonate spheres are added to such a protein solution, their pores are filled with the insulin solution. When the solution is then neutralized with acid, the insulin becomes insoluble and precipitates out in the pores. If the solution is acidified further, until the calcium carbonate spheres slowly begin to dissolve in the slightly acidic solution. The insulin remains behind as a loose matrix, which shrinks down into compact micrometer-scale spheres. This results in protein particles of uniform size and high protein density.

Author: Dmitry V. Volodkin, Technische Universität Berlin (Germany),
www.chemie.tu-berlin.de/klitzing/menue/ueber_uns/arbeitsgruppe/volodkin/

Title: Pure Protein Microspheres by Calcium Carbonate Templating

Angewandte Chemie International Edition, Permalink to the article:dx.doi.org/10.1002/anie.201005089

####

For more information, please click here

Copyright © Angewandte Chemie International Edition

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

'Exotic' material is like a switch when super thin April 18th, 2014

Innovative strategy to facilitate organ repair April 18th, 2014

Oxford Instruments Asylum Research Introduces the MFP-3D InfinityTM AFM Featuring Powerful New Capabilities and Stunning High Performance April 18th, 2014

Conductive Inks: booming to $2.8 billion by 2024 April 17th, 2014

Nanomedicine

Innovative strategy to facilitate organ repair April 18th, 2014

Novel stapled peptide nanoparticle combination prevents RSV infection, study finds April 17th, 2014

More effective kidney stone treatment, from the macroscopic to the nanoscale April 17th, 2014

High-temperature plasmonics eyed for solar, computer innovation April 17th, 2014

Announcements

'Exotic' material is like a switch when super thin April 18th, 2014

Innovative strategy to facilitate organ repair April 18th, 2014

Oxford Instruments Asylum Research Introduces the MFP-3D InfinityTM AFM Featuring Powerful New Capabilities and Stunning High Performance April 18th, 2014

Transparent Conductive Films and Sensors Are Hot Segments in Printed Electronics: Start-ups in these fields show above-average momentum, while companies working on emissive displays such as OLED are fading, Lux Research says April 17th, 2014

Nanobiotechnology

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone April 15th, 2014

In latest generation of tiny biosensors, size isn't everything: UCLA researchers overturn conventional wisdom on nanowire-based diagnostic devices April 11th, 2014

Virus structure inspires novel understanding of onion-like carbon nanoparticles April 10th, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE